ABSTRACT
The permanent action of small doses of low-intensity radiation on the immune status of 2.5-3.5 month CC57W mice has been investigated. Total doses of internal and external irradiation were about few cGy. The permanent action of low-level radiation on the experimental animals of the first and fourth generations was shown to change spleen and lymph nodes weights and the count of lymphocytes isolated from these organs. Cellularity and DNA synthesis in the lymph-node lymphocytes and their proliferative response to polyclonal mitogens also changed. The alterations in the parameters that characterized T-lymphocyte population were statistically significant.
Subject(s)
Accidents , Immune System/radiation effects , Immunocompetence/radiation effects , Nuclear Reactors , Power Plants , Animals , Body Weight/radiation effects , Dose-Response Relationship, Radiation , Environmental Exposure , Immune System/pathology , Male , Mice , Mice, Inbred C57BL , Organ Size/radiation effects , Time Factors , UkraineABSTRACT
The immune status of mice has been assessed by the whole complex of data. The permanent action of low-level radiation has been shown to suppress considerably the rate of reactions of the delayed-type hypersensitivity and "graft versus host" disease, as well as NK and specific cytolytic T-lymphocyte activity. The dynamics of accumulation and the levels of antiviral antibodies in the serum, lung and trachea extracts are virtually invariable. The resistance of experimental animals to influenza is lower than that of non-irradiated mice of the same line and age. The data obtained indicate that the immune disturbances revealed are connected not only with the alteration of lymphoid cell populations, but also with the alteration of the immune regulation mechanisms.
Subject(s)
Accidents , Aging/radiation effects , Lymphocyte Cooperation/radiation effects , Nuclear Reactors , Power Plants , Aging/immunology , Animals , Environmental Exposure , Female , Graft vs Host Reaction/immunology , Graft vs Host Reaction/radiation effects , Hypersensitivity, Delayed/immunology , Immunity, Innate/immunology , Immunity, Innate/radiation effects , Influenza A virus , Lymphocyte Cooperation/immunology , Male , Mice , Mice, Inbred C57BL , Orthomyxoviridae Infections/immunology , UkraineABSTRACT
A study was made of the effect of injection of cyclophosphamide (CP) immunomodulating to BALB/c mice, according to 3 schemes of injection with the whole dose being 200 mg/kg. In all, the state of peripheral blood and spleen, the cellular content of lymph nodes and the number of DNA-synthesizing cells in them were studied, in addition to the ability of lymph node lymphocytes to proliferative reaction on T and B cells mitogens in vivo. It was determined that a 2-fold injection of CP, in contrast to 10- and 5-fold injections, resulted in splenomegaly and leukocytosis in the experimental animals. By means of quantitative tests in vivo, in was demonstrated that the dynamics of restoration of proliferative responses to mitogens depends on the CP injection scheme. These tests proved to be more informative for registration of immunomodulating effect of CP, than the study of cellular content of lymph nodes and the number of DNA-synthesizing cells in them.
Subject(s)
Adjuvants, Immunologic , Cyclophosphamide/administration & dosage , Animals , B-Lymphocytes/drug effects , B-Lymphocytes/immunology , Concanavalin A/pharmacology , DNA/biosynthesis , Dextran Sulfate , Dextrans/pharmacology , Female , Leukocyte Count , Lymph Nodes/drug effects , Lymph Nodes/immunology , Mice , Mice, Inbred BALB C , Mitogens/pharmacology , Spleen/drug effects , Spleen/immunology , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , Time FactorsABSTRACT
A comparative morphofunctional analysis of lymphoid cells of BALB/c mice with the transplantable tumours was performed after the cyclophosphamide (CP) treatment. CP was injected by 3 schemes in a dose of 200 mg/kg of body weight. It was found that the more effective therapeutic scheme of CP treatment is accompanied by essential normalization of the ratio of lymphocyte populations in the lymph nodes against a background of a comparatively good state of proliferating cells as well as by a higher rate of restoration of proliferative responses to T and B cell mitogens in vivo.
Subject(s)
B-Lymphocytes/immunology , Cyclophosphamide/therapeutic use , Sarcoma, Experimental/drug therapy , Soft Tissue Neoplasms/drug therapy , T-Lymphocytes/immunology , Adjuvants, Immunologic , Animals , Female , Leukocyte Count , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Sarcoma, Experimental/immunology , Soft Tissue Neoplasms/immunologyABSTRACT
Cytological examinations and evaluation of the proliferative response of lymph node lymphocytes to T- and B-cell mitogens in vivo were carried out to identify the immunomodulating effect of cyclophosphamide (CP) versus the scheme of treatment. Using the system of adoptive transfer of splenic lymphocytes from experimental animals to intact syngeneic recipients, it was found that the scheme-dependent differentiated effects of CP on the antigen-specific tumor-associated and nonspecific suppressive activity of spleen cells provides an important pathway of CP action on the organism. A relationship between CP immunomodulating effects and lymphocyte antitumor activity was demonstrated in experiments using diffusion chambers.
Subject(s)
Adjuvants, Immunologic , Cyclophosphamide/administration & dosage , Animals , Drug Evaluation, Preclinical , Female , Immunization, Passive , Leukocytes/drug effects , Leukocytes/immunology , Lymph Nodes/drug effects , Lymph Nodes/immunology , Lymphocytes/drug effects , Lymphocytes/immunology , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Sarcoma, Experimental/drug therapy , Sarcoma, Experimental/immunology , Spleen/drug effects , Spleen/immunology , Time FactorsABSTRACT
The suppressor activity of the splenic cells in the cyclophosphamide (CP)-treated BALB/c mice with transplanted tumours was studied. CP was injected according to three different therapeutic effective schedules. The analysis of non-specific and antigen-specific tumour-associated suppressor activity in the splenic cell adoptive transfer system demonstrated that CP immunomodulating influence depends on the injection schedule. Usage of the most therapeutically effective CP injection schedule ensured a relative safety of the nonspecific tumour-associated suppressor activity and depression of the specific one.
Subject(s)
Cyclophosphamide/therapeutic use , Sarcoma, Experimental/drug therapy , Spleen/drug effects , T-Lymphocytes, Regulatory/drug effects , Animals , Concanavalin A/pharmacology , Female , Immunity, Innate/drug effects , Immunization, Passive , Methylcholanthrene , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Sarcoma, Experimental/chemically induced , Sarcoma, Experimental/immunology , Spleen/immunology , T-Lymphocytes, Regulatory/immunology , Time Factors , Transplantation, IsogeneicABSTRACT
Fibroadenomatous tissue of the human mammary gland was cultivated in diffuse chambers implanted into mice. On day 6 of culture the growing cells were subjected to morphological and autoradiographic analysis. The index of 3H-thymidine labeling of cell nuclei was found to correlate with the morphological pattern of dyshormonal fibroadenomatosis of the mammary gland. It is discussed whether it is desirable to use the culture in diffuse chambers for screening the actively proliferating forms human mammary gland dyshormonal dysplasias prone to malignancy.
Subject(s)
Breast Diseases/pathology , Fibrocystic Breast Disease/pathology , Cell Division , Culture Techniques/instrumentation , Female , HumansABSTRACT
The ratio of T and B lymphocytes and DNA content in population enriched by these cells were analysed in the lymph nodes of BALB/c mice with progressively growing sarcomas induced by the MSV Moloney and in untreated mice. T lymphocyte enriched fractions have a higher DNA content in comparison to fractions enriched with B lymphocytes. Sarcoma development promotes a decrease in the relative number of macronucleolar lymphocytes and simultaneous decrease of micronucleolar ones in the lymph nodes, particularly in the regional ones.
