[Leukemogenesis and bone marrow hematopoietic disorders in baboons with immunoblastic malignant lymphomas of B- and T-cell types]. / Leikemizatsiia i narusheniia kostnomozgovogo krovetvoreniia u pavianov pri immunoblastnykh zlokachestvennykh limfomakh B- i T-kletochnogo tipa.

Leukemization in baboon B- and T-cell immunoblastomas was investigated. Immunologic identification of tumor was carried out using standard immunological and cytochemical tests, e. g. identification of several types of E-rosette forming lymphocytes, surface and cytoplasmic immunoglobulin-carrying lymphocytes and assay of such acid hydrolases as acid phosphatase, acid alpha-naphthyl acetate esterase and beta-glucuronidase in lymphoid cells. Leukemization of immunoblastomas with bone marrow infiltration by tumor immunoblasts and the appearance of the latter in peripheral blood were observed rather frequently--in 7 of 10 cases of B-cell and in all 4 cases of T-cell immunoblastoma. Immunoblastoma progression was accompanied by a rise in lymphoid element level in peripheral blood and a lesser increase in bone marrow. At terminal stage, both patterns of immunoblastoma sometimes involved the development of leukemoid reactions of a myeloid type and disturbances in bone marrow hematopoiesis which manifested themselves by mild anemia and a slightly pronounced failure to produce platelets.

[Ultrastructural characteristics of malignant non-Hodgkin's lymphoma in baboons]. / Ul'trastrukturnaia kharakteristika zlokachestvennykh nekhodzhinskikh limfom pavianov.

The ultrastructure of 29 malignant non-Hodgkin's lymphomas of baboons including prolymphocytic lymphoplasmacytic, lymphoblastic, and immunoblastic malignant lymphomas is described. The ultrastructure of the cells of B-cell malignant lymphomas is characterized by a more developed strands system of rough endoplasmic reticulum in the tumor cell cytoplasm and the presence of structural abnormalities in the protein synthesis in some B-cell malignant lymphoma cells in the form of Russell's and Dutcher bodies. Analogous baboon and human tumors were shown to have a high degree of cell ultrastructure similarity. Rare forms of baboon malignant non-Hodgkin's lymphomas including a large-cell variant of T-cell lymphoblastic lymphoma with large multilobated nuclei and a variety of B-cell lymphoma with an intermediate degree of cell differentiation have been found. Malignant lymphoma of large multilobated T-cell type and lymphoma of the intermediate lymphocytic type occur rarely in human pathology too.

The establishment of the suspension Epstein-Barr virus producing cell lines from patients with tumoral diseases.

Isolation of the Epstein-Barr virus (EBV) in suspension lymphoblastoid cell lines from human patients with tumor diseases, mainly malignant lymphoma, has been described. It has been shown that the EBV was isolated from human patients with myeloid type of leukemia in 75% of cases. A similar virus was also isolated from patients with Hodgkin's disease and leukemoid reaction of the myeloid type for lung cancer. Morphological, cytochemical, immunological, and cytogenetic characteristics of the cell lines in which the EBV is replicated have been investigated.