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Mikrobiol Z ; 74(2): 67-72, 2012.
Article in Ukrainian | MEDLINE | ID: mdl-22686021

ABSTRACT

To reveal Herpes simplex virus 2 specific IgG and to determine their avidity the ELISA test-kit was constructed using recombinant protein gG2 (PSC SPC Diaproph-Med). Using this test-kit the distribution of specific antibodies with different avidity indexes was investigated in practically healthy donor samples. It is possible to use the mentioned ELISA test-kit for confirmation of primary infection, and also for differentiation of primary infection, chronic disease and virus reactivation. Thus, this ELISA test-kit could be an additional tool in herpetic infection diagnosis.


Subject(s)
Antibodies, Viral , Antibody Affinity/immunology , Herpes Genitalis/diagnosis , Herpesvirus 2, Human/physiology , Immunoglobulin G , Antibodies, Viral/immunology , Chronic Disease , Cloning, Molecular , Enzyme-Linked Immunosorbent Assay , Escherichia coli , Herpes Genitalis/immunology , Herpes Genitalis/virology , Humans , Immunoglobulin G/immunology , Plasmids , Reagent Kits, Diagnostic , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Severity of Illness Index , Virus Activation
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