[Ceruloplasmin and peptides of medium molecular weight as parameters in development of acute myeloblastic leukemia]. / Tseruloplazmin i srednemolekuliarnye petidy kak kriterii techeniia ostrogo mieloblastnogo leikoza.

A dynamic content of ceruloplasmin and medium-molecular peptides were studied in the blood serum of 28 patients with an acute clinical course of myeloblast-type leucosis during a scheduled chemotherapy. The mentioned biochemical criteria were shown to be able to characterize indirectly an intensity of the clinical course, functional liver condition and the efficiency of chemotherapy.

[Effect of cryoconservation of platelets on polyamine level and cathepsin D activity]. / Vliianie kriokonservirovaniia trombotsitov na uroven' poliaminov i aktivnost' katepsina D.

Alterations in activity of cathepsin D as well as in content of polyamines spermine, spermidine, x-fraction (apparently, acetyl spermidine) and putrescine were studied in blood platelets depending on conditions of conservation. The enzyme activity and content of polyamines correlated directly with duration of the cell concentrates storage. Cathepsin D and polyamines appear to be involved in responses to stress adaptation.

[Biochemical and cytochemical criteria for predicting the course of acute lymphoblastic leukemia in children]. / Biokhimicheskie i tsitokhimicheskie kriterii prognozirovaniia techeniia ostrogo limfoblastnogo leikoza u detei.

Cytochemical parameters of intracellular metabolism of leukemia cells and bone marrow polyamines were studied in 113 acute lymphoblastic leukemia patients aged from 1 to 14 years. The parameters analyzed characterized the severity of the disease course, cytostatic therapy effectiveness and primary resistance. It is shown that determination of biochemical and cytochemical criteria before the beginning of acute lymphoblastic leukemia therapy may be conducive to the correct choice of treatment.

[Putrescine in evaluation of the leukemic process in children]. / Putrescin v otsenke aktivnosti leikoznogo protsessa u detei.

Relationship between elevated putrescine level in urine and its presence in the blood and bone marrow was analyzed in 44 children with acute leukemia in the active stage. It has been shown that putrescine is a marker of activation of leukemic proliferation that is confirmed by the disease course character in patients with elevated putrescine level in urine (66.3 +/- 5.1 mumols/day) and its presence in the blood and bone marrow. Putrescine can serve as an early precursor of relapses during investigation of patients in the remission state.

[Levels of extracellular DNA and polyamines as criteria of resistance to chemotherapy in patients with acute leukemia]. / Urovni vnekletochnoi DNK i poliaminov kak pokazateli refrakternosti k khimioterapii bol'nykh ostrymi leikozami.

Content of polyamines and extracellular DNA were studied in blood of patients with acute leukoses which did not respond to chemotherapy. Concentration of total polyamines was distinctly lower in blood of patients with acute lymphoblast and myeloblast leukoses as compared with healthy persons. Free and total polyamines were unaltered in blood of these patients after cytostatic therapy. In the patients with acute leukoses content of extracellular DNA was 2-fold higher as compared with controls. The high DNA content was maintained during the therapeutic course. Absence of any alterations in the biochemical patterns studied suggest that the patients with acute leukoses did not respond to the course of treatment used.

[Polyamines as a prognostic criterion of the duration of clinico-hematologic remission in acute leukemia in children]. / Poliaminy kak prognosticheskii kriterii dlitel'nosti kliniko-gematologicheskoi remissii pri ostrom leikoze u detei.

The authors reported the results of retrospective analysis of the level of polyamines in urine and the duration of clinicohematological remission in 25 AL children aged 3 to 14. Of this number 23 patients suffered from acute lymphoblastic leukemia, 2--from acute myeloblastic leukemia. Direct correlation between polyamine changes during a course of chemotherapy and the duration of remission was established. Longer duration was observed in the patients in whom a 2-fold rise of the level of all polyamines, especially spermidine, followed the administration of a cytostatic within the first 24-72 h. On the discontinuation of therapy a certain decrease in polyamine excretion with urine was observed in these children as compared to the level determined on their admission to hospital.