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Mol Biol (Mosk) ; 21(4): 1060-9, 1987.
Article in Russian | MEDLINE | ID: mdl-3309618

ABSTRACT

The efficiency of hybrid promotor Ptac', comprising a synthetic trp-promoter and lacUV5 "-10" sequence, was studied. By means of electrophoresis and hybridization of RNA-products obtained in vitro and in minicells, with promotor-containing plasmids; the hybrid promotor was found to be 6 times and 3-4 times as efficient as trp and lacUV5 promoters, respectively.


Subject(s)
Genetic Engineering , Nucleic Acid Hybridization , Promoter Regions, Genetic , Escherichia coli/genetics , Lac Operon , Plasmids , Tryptophan/genetics
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