Identification and evolutionary analysis of papillomavirus sequences in New World monkeys (genera Sapajus and Alouatta) from Argentina.

OBJECTIVE: In this study, we investigated the occurrence of papillomavirus (PV) infection in non-human primates (NHPs) in northeastern Argentina. We also explored their evolutionary history and evaluated the co-speciation hypothesis in the context of primate evolution. METHODS: We obtained DNA samples from 57 individuals belonging to wild and captive populations of Alouatta caraya, Sapajus nigritus, and Sapajus cay. We assessed PV infection by PCR amplification with the CUT primer system and sequencing of 337 bp (112 amino acids) of the L1 gene. The viral sequences were analyzed by phylogenetic and Bayesian coalescence methods to estimate the time to the most common recent ancestor (tMRCA) using BEAST, v1.4.8 software. We evaluated viral/host tree congruence with TreeMap v3.0. RESULTS: We identified two novel putative PV sequences of the genus Gammapapillomavirus in Sapajus spp. and Alouatta caraya (SPV1 and AcPV1, respectively). The tMRCA of SPV1 was estimated to be 11,941,682 years before present (ybp), and that of AcPV1 was 46,638,071 ybp, both before the coalescence times of their hosts (6.4 million years ago [MYA] and 6.8 MYA, respectively). Based on the comparison of primate and viral phylogenies, we found that the PV tree was no more congruent with the host tree than a random tree would be (P > 0.05), thus allowing us to reject the model of virus-host coevolution. CONCLUSION: This study presents the first evidence of PV infection in platyrrhine species from Argentina, expands the range of described hosts for these viruses, and suggests new scenarios for their origin and dispersal.

Influence of extraordinary floods on wildlife parasites: the case of gastrointestinal helminths and protozoa of wild canids from the Iberá Ecoregion, Argentina.

Parasites are natural components of ecosystems and play a significant role in the dynamics of wild animal populations. Although the environment of parasites is primarily defined by the host, most life cycles involve stages that must endure external conditions. Rainfall and flooding events are important factors that might influence the transport of parasitic stages, altering soil moisture levels, and resulting in a favorable environment for parasite survival and development. We assessed whether an extraordinary flood event modified the occurrence of gastrointestinal parasites (nematodes and protozoa) in wild canids in two protected areas in northern Argentina. From 2016 to 2018, we collected fecal samples of two fox species, Lycalopex gymnocercus and Cerdocyon thous, and examined the presence of nematodes and protozoa. We assessed changes in the occurrence of these parasites after a flood event, while adjusting for potential confounders (i.e., monthly average temperature, season, host species, site). In a second stage of the analysis, we evaluated whether part of the effect was caused by changes in soil moisture, by adding normalized difference water index as an independent variable. We found that the presence of nematodes in foxes was higher after flooding than before flooding, and this association was not explained by changes in the soil moisture. On the other hand, the flood event was not relevant for protozoa. Stronger and long-lasting flood events are expected due to the effect of global warming on El Niño events, and this may increase and intensify the spread of some parasites affecting wildlife, which could also be of public health concern.

Helminth infracommunity in a maned wolf, Chrysocyon brachyurus, from the humid Chaco, Argentina.

Parasitism is an important factor in conservation worldwide, especially for endangered species, as it can affect host populations by reducing growth rates, fecundity and affecting nutritional status. The maned wolf Chrysocyon brachyurus, a near-threatened species, is distributed across different habitats from the south Amazonian forest in Brazil to northern Argentina, Paraguay and eastern Bolivia. Most studies on gastrointestinal parasites in wild maned wolves are based on coprological findings, and there is little information about adult parasites and parasite species richness. Therefore, this study aims to expand the knowledge about the helminth infracommunity of a maned wolf, describe adult parasites, and explore parasitic coinfections. We performed a necropsy of an adult individual found road killed in Chaco province, Argentina. We collected adult worms from subcutaneous tissues, from the right ventricle, kidneys, and intestine. The parasites were morphologically identified as Dirofilaria immitis, Dioctophyma renale and Spirometra sp. respectively. To our knowledge, this is the first record for adults of Dirofilaria immitis in maned wolves. Filarioids were located within the heart as well as in subcutaneous tissues with mature females containing uterine microfilariae; this study also adds C. brachyurus as a new host for Spirometra sp. in Argentina. Considering that the three helminths found herein are zoonotic and that the maned wolf is an endangered canid, it is essential provide baseline information to determine the risk factors involved in the transmission and extend the studies to sympatric wild and domestic canids in the area.

Phlebotominae in peri-domestic and forest environments inhabited by Alouatta caraya in northeastern Argentina.

Multiple species of Phlebotominae are vectors of Leishmania (Protozoa: Trypanosomatidae), which causes visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). To describe the Phlebotominae (Diptera: Psychodidae) related to the environments of black and gold howler monkeys Alouatta caraya (Humbodlt, 1812) (Primates: Atelidae), potential vectors were sampled in different landscapes and vertical strata of sleeping trees. Phlebotomine captured between December 2011 and March 2012 (2365 individuals) belonged to eight species, of which Nyssomyia neivai (Pinto, 1926) (61.4%) and Migonemyia migonei (França, 1920) (18.73%) were the most abundant, and Ny. withmani was recorded for the first time in the Chaco province. In the 'peri-domestic' landscape, the phlebotomine were mainly captured in henhouses (78.7%), whereas the tree canopy in 'rural' and 'wild' landscapes yielded 31.2% and 29.1% of the phlebotomine, respectively. A significant association between the type of landscape and the species of phlebotomine was observed by multivariate analysis. Lutzomyia longipalpis (Lutz & Neiva, 1912) and Mg. migonei were associated with 'peri-domestic' landscape, and Ny. neivai was associated with the 'wild' landscape. The results of this prospective study suggest that the interaction between phlebotomine and A. caraya could be a key factor with respect to understanding the epidemiology of leishmaniasis.