Possibility of Using Wind Turbine Waste in Particleboard Manufacturing.

Recent reports indicate that the development of electricity generation using wind turbines will continue to grow. Despite the long service life of wind turbine blades, their technological life comes to an end at a certain point. Currently, there is no industrial method for recycling them, and the proposed solutions need to consider a complete and comprehensive approach to this material. In many countries, these blades are stored in special landfills and await proposals for rational recycling. It has been proposed that this recyclable yet still troublesome raw material be used in building sheathing boards. Sheathing boards used in the construction industry have a relatively long lifecycle. Three types of polymer chips and two resins, i.e., PF and MUF, were used in the study. The boards' quality was assessed per the standards specified for particle boards. The resulting boards were characterized by strengths above 20 N/mm2 and an elastic modulus close to 4000 N/mm2. Slightly better results were obtained with the MUF resin.

Influence of the Structure of Lattice Beams on Their Strength Properties.

This paper presents the strength properties of wooden trusses. The proposed solutions may constitute an alternative to currently produced trusses, in cases when posts and cross braces are joined with flanges using punched metal plate fasteners. Glued carpentry joints, although requiring a more complicated manufacturing process, on the one hand promote a more rational utilisation of available structural timber resources, while on the other hand they restrict the use of metal fasteners. The results of the conducted analyses show that the proposed solutions at the current stage of research are characterised by an approx. 30% lower static bending strength compared to trusses manufactured using punched metal plate fasteners. However, these solutions make it possible to produce trusses with load-bearing capacities comparable to that of structural timber of grade C24 and stiffness slightly higher than that of lattice beams manufactured using punched metal plate fasteners. The strength of wooden trusses manufactured in the laboratory ranged from nearly 20 N/mm2 to over 32 N/mm2. Thus, satisfactory primary values for further work were obtained.

The Usefulness of Pine Timber (Pinus sylvestris L.) for the Production of Structural Elements. Part I: Evaluation of the Quality of the Pine Timber in the Bending Test.

The study assessed the quality of pine lumber by marking the modulus of elasticity in the horizontal system. The research material was a plank with the following dimensions: 137 mm wide × 39.50 mm thick × 3485 mm long. The pine wood was obtained by sawing timber in the form of logs with round cross sections and originating from the Forest Division Olesno (50°52'30″ N, 18°25'00″ E). Each long log was sawn to provide four logs of about 3.5 m, which were marked as butt-end logs (O), middle logs (S)-2 items, and top logs (W). The origin of the logs from the trunk (Pinus sylvestris L.) has a significant impact on the physical and mechanical properties of the wood from which they are made. Only butt-end logs (log type O) allows for the production of high-quality timber elements. The pine timber that was evaluated in this paper had a high density of about 570 kg/m3 and a high percentage of timber items were assigned to class C24 and higher (above 50%). The adopted horizontal model of evaluation of the modulus of elasticity gave similar results to those obtained in an evaluation according to the EN-408.

The Usefulness of Pine Timber (Pinus sylvestris L.) for the Production of Structural Elements. Part II: Strength Properties of Glued Laminated Timber.

The paper assessed the feasibility of manufacturing glued structural elements made of pine wood after grading it mechanically in a horizontal arrangement. It was assumed that the pine wood was not free of defects and that the outer lamellas would also be visually inspected. This would result in only rejecting items with large, rotten knots. Beams of the assumed grades GL32c, GL28c and GL24c were made of the examined pine wood. Our study indicated that the expected modulus of elasticity in bending was largely maintained by the designed beam models but that their strength was connected with the quality of the respective lamellas, rather than with their modulus of elasticity. On average, the bending strength of the beams was 44.6 MPa. The cause of their destruction was the individual technical quality of a given item of timber, which was loosely related to its modulus of elasticity, assessed in a bending test. Although the modulus of elasticity of the manufactured beam types differed quite significantly (11.45-14.08 kN/mm2), the bending strength for all types was similar. Significant differences occurred only during a more detailed analysis because lower classes were characterized by a greater variation of the bending strength. In this case, beams with a strength of 24 MPa to 50 MPa appeared.

Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation.

Restriction endonucleases naturally target DNA duplexes. Systematic screening has identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes and that may therefore be useful as tools for RNA biochemistry. We have chosen AvaII (G↓GWCC, where W stands for A or T) as a representative of this group of restriction endonucleases for detailed characterization. Here, we report crystal structures of AvaII alone, in specific complex with partially cleaved dsDNA, and in scanning complex with an RNA/DNA hybrid. The specific complex reveals a novel form of semi-specific dsDNA readout by a hexa-coordinated metal cation, most likely Ca2+ or Mg2+. Substitutions of residues anchoring this non-catalytic metal ion severely impair DNA binding and cleavage. The dsDNA in the AvaII complex is in the A-like form. This creates space for 2'-OH groups to be accommodated without intra-nucleic acid steric conflicts. PD-(D/E)XK restriction endonucleases of known structure that bind their dsDNA targets in the A-like form cluster into structurally similar groups. Most such enzymes, including some not previously studied in this respect, cleave RNA/DNA heteroduplexes. We conclude that A-form dsDNA binding is a good predictor for RNA/DNA cleavage activity.

Activity and structure of EcoKMcrA.

Escherichia coli McrA (EcoKMcrA) acts as a methylcytosine and hydroxymethylcytosine dependent restriction endonuclease. We present a biochemical characterization of EcoKMcrA that includes the first demonstration of its endonuclease activity, small angle X-ray scattering (SAXS) data, and a crystal structure of the enzyme in the absence of DNA. Our data indicate that EcoKMcrA dimerizes via the anticipated C-terminal HNH domains, which together form a single DNA binding site. The N-terminal domains are not homologous to SRA domains, do not interact with each other, and have separate DNA binding sites. Electrophoretic mobility shift assay (EMSA) and footprinting experiments suggest that the N-terminal domains can sense the presence and sequence context of modified cytosines. Pyrrolocytosine fluorescence data indicate no base flipping. In vitro, EcoKMcrA DNA endonuclease activity requires Mn2+ ions, is not strictly methyl dependent, and is not observed when active site variants of the enzyme are used. In cells, EcoKMcrA specifically restricts DNA that is modified in the correct sequence context. This activity is impaired by mutations of the nuclease active site, unless the enzyme is highly overexpressed.

The assessment of renal function in patients with newly diagnosed hypertension - the role of hyperuricemia as a risk factor for chronic kidney disease - preliminary study.

For the last few years, the role of uric acid in the pathogenesis of the hypertension and chronic kidney disease has drawn attention due to the increasing prevalence of the aforementioned states. Uric acid (UA), formed as a result of purine metabolism, is subject in the renal proximal tubule to filtration, reabsorption and secretion. The mechanisms of deleterious effect of UA on the kidney involve endothelial damage and the increment of hydrostatic pressure in the glomerulus which in turn lead to hypertrophy and glomerulosclerosis. Moreover, abnormally elevated levels of uric acid results in the activation of renin-angiotensinaldosterone system and thus it may be associated with disturbances of water and electrolyte balance and the appearance of hypertension. AIM: The aim of this study was to assess the prevalence of hyperuricemia in patients with newly diagnosed hypertension and its effect on renal function. MATERIALS AND METHODS: This is a retrospective study including 315 hypertensive patients (157 women - 49.8% and 158 men - 50.2%, aged 39.3 ± 14.0 years) hospitalized in the Department of Nephrology, Hypertension and Family Medicine, WAM University Hospital of Lodz (study group) and 181 individuals without hypertension (103 women - 56.9% and 78 men - 43.1%, aged 42.4 ± 14.0) (control group). RESULTS: Hyperuricemia was observed in 6.7% of patients with newly diagnosed hypertension. A positive correlation between serum creatinine and uric acid (Spearman coefficient 0.426, p<0.0001) and negative correlation between uric acid and eGFR (Spearman coefficient -0.116, p =0.068) were demonstrated in this study. CONCLUSIONS: Patients with newly diagnosed hypertension have higher levels of uric acid in comparison to control group. Elevated levels of uric acid can influence the development of hypertension and aggravate hypertension-associated renal damage.

Quality of life in heart failure patients undergoing home-based telerehabilitation versus outpatient rehabilitation--a randomized controlled study.

BACKGROUND: The most common manifestation of heart failure is physical capacity impairment resulting in dyspnoea and fatigue. The disease deteriorates the quality of life (QoL). Its consequences restrict not only functioning in the physical aspect of QoL but also patients' emotional condition. OBJECTIVE: The study is aimed to assess QoL changes in HF patients after home-based telemonitored cardiac rehabilitation (HTCR Group) versus outpatient-based standard cardiac rehabilitation (SCR Group). METHODS: The study comprised 131 heart failure patients (aged 56.4±10.9 years; II/III NYHA) rehabilitated for eight weeks in two random groups: HTCR Group (n=75), participating in home-based rehabilitation supervised telemedically based on walking training; SCR Group (n=56), participating in traditional outpatient-based rehabilitation (cycloergometer training). QoL parameters were assessed with a Polish version of the Medical Outcomes Study Short Form 36 Health Survey Questionnaire (SF-36). RESULTS: After rehabilitation, both groups achieved a significant QoL improvement, both physically and mentally. HTCR Group patients improved in QoL physical categories in one subscale (physical function), and in two subscales in the mental categories (mental health, vitality). In SCR Group, three physical subscales improved (physical function, role limitation caused by physical problems, bodily pain). In the mental categories, also three subscales improved (social function, mental health, vitality). CONCLUSIONS: The study demonstrated that in heart failure patients HTCR provided a similar improvement in total QoL index as SCR. Yet it differed in QoL subscales. Patients who underwent home-based telerehabilitation observed an improvement mainly in the mental categories. Patients in SCR Group improved their general physical well-being.

Crystal structure of the 5hmC specific endonuclease PvuRts1I.

PvuRts1I is a prototype for a larger family of restriction endonucleases that cleave DNA containing 5-hydroxymethylcytosine (5hmC) or 5-glucosylhydroxymethylcytosine (5ghmC), but not 5-methylcytosine (5mC) or cytosine. Here, we report a crystal structure of the enzyme at 2.35 Å resolution. Although the protein has been crystallized in the absence of DNA, the structure is very informative. It shows that PvuRts1I consists of an N-terminal, atypical PD-(D/E)XK catalytic domain and a C-terminal SRA domain that might accommodate a flipped 5hmC or 5ghmC base. Changes to predicted catalytic residues of the PD-(D/E)XK domain or to the putative pocket for a flipped base abolish catalytic activity. Surprisingly, fluorescence changes indicative of base flipping are not observed when PvuRts1I is added to DNA substrates containing pyrrolocytosine in place of 5hmC (5ghmC). Despite this caveat, the structure suggests a model for PvuRts1I activity and presents opportunities for protein engineering to alter the enzyme properties for biotechnological applications.

Membrane potential-dependent binding of polysialic acid to lipid monolayers and bilayers.

Polysialic acids are linear polysaccharides composed of sialic acid monomers. These polyanionic chains are usually membrane-bound, and are expressed on the surfaces of neural, tumor and neuroinvasive bacterial cells. We used toluidine blue spectroscopy, the Langmuir monolayer technique and fluorescence spectroscopy to study the effects of membrane surface potential and transmembrane potential on the binding of polysialic acids to lipid bilayers and monolayers. Polysialic acid free in solution was added to the bathing solution to assess the metachromatic shift in the absorption spectra of toluidine blue, the temperature dependence of the fluorescence anisotropy of DPH in liposomes, the limiting molecular area in lipid monolayers, and the fluorescence spectroscopy of oxonol V in liposomes. Our results show that both a positive surface potential and a positive transmembrane potential inside the vesicles can facilitate the binding of polysialic acid chains to model lipid membranes. These observations suggest that these membrane potentials can also affect the polysialic acid-mediated interaction between cells.

Self-perception of women after mastectomy as an ego defence mechanism. Comparison with a group of healthy women.

AIM OF THE STUDY: Analysis of changes in self-perception in post-mastectomy patients and its comparison with self-perception of healthy women. MATERIAL AND METHODS: The subjects of this study were 50 women. The main group was post-mastectomy patients involved in the meetings of the Amazons Club (25 women). The reference group consisted of 25 healthy women. The method used in the study was the ACL (Adjective Check List) test, identifying 37 dimensions of self-image. Oncological patients completed a test twice (for current and pre-cancer self-image), and healthy women once - for current self. Both groups were selected similarly in respect of education level for the purpose of ensuring a similar level of insight. RESULTS: Retrospective self-image and the current one in the Amazon women group were highly convergent. Existing differences include a reduced need for achievement and dominance, and a lower level of self-confidence. However, the comparison of current self-images in both groups showed a large discrepancy of the results. The Amazon women assess themselves in a much more negative way. Also, their self-image is self-contradictory in certain characteristics. CONCLUSIONS: Mastectomy is a difficult experience requiring one to re-adapt and to accept oneself thereafter. The way of thinking about oneself is a defence mechanism helping to cope. The work with patients programmes must, therefore, focus on identifying their emotions and thoughts, especially on those they do not want to accept because of the perceived pressure from the environment to effectively and quickly deal with this difficult situation. The increasing acceptance of personal limitations may help the affected women to adjust psychologically faster and easier.

Making GRADE accessible: a proposal for graphic display of evidence quality assessments.

When generating guidelines, quality of evidence is frequently reported in tabulated form capturing several domains, for example, study design, risk of bias and heterogeneity. Increasingly, this is done using the Grading of Recommendations Assessment, Development and Evaluation approach. As assimilating large amount of tabulated data across several comparisons and outcomes spread over many pages (sometimes hundreds) is not easy, there is a need to present evidence summaries in a more effective way. A graphic display plotting the several domains used in evidence grading on equiangular spokes starting from the same point, the data length of each spoke proportional to the magnitude of the quality, succinctly captures tabulated information. These plots allow easy identification of deficiencies, outliers and similarities in evidence quality for individual and multiple comparisons and outcomes, paving the way for their routine use alongside tabulated information.

Enhancing intentions to attend cervical cancer screening with a stage-matched intervention.

OBJECTIVE: The study evaluated the effects of a pros enhancing intervention on intention to uptake cervical cancer screening. It was hypothesized that the pros enhancement session (compared to an education session) would affect intentions of preintentional women, whereas it was expected to have negligible effects among women in intentional and actional stages of the health action process approach (HAPA). Thus, we tested the HAPA using stage-matched and stage-mismatched interventions. Further, a change in decisional balance was assumed to mediate the relationship between the group assignment and intention, with age acting as the moderator. DESIGN AND METHODS: Respondents (1,436 women, aged 18-60) were randomly assigned to either the intervention or control condition and filled out questionnaires before and directly after the manipulation (in one web-based session). RESULTS: Direct effects of the group assignment were observed only among preintentional women. Across the stages, however, change in decisional balance mediated the effects of the group assignment on the intention to uptake screening. Further, among participants in the preintentional stage, this mediation became significant only for women aged 35 or older. CONCLUSIONS: Although direct effects are in line with stage assumptions of the HAPA, meditational analysis among pre- and post-intentional women indicated that similar processes accounted for post-manipulation intention. Future research testing stage models should account for the mediating processes, which explain the effects of the intervention.

Psychological support for patients following myocardial infarction.

The paper presents the principal psychological problems in patients following myocardial infarction. Particular emphasis has been placed on anxiety and depression following myocardial infarction and behavioural patterns adversely affecting health. A proposal of actions during cardiac rehabilitation has been presented in accordance with the severity of psychological problems encountered in the patients.

[Placebo: its hypotensive effect and influence on certain psychological parameters and dependency on dosage prescribed to patients]. / Placebo--jego dzialanie hipotensyjne oraz wplyw na wybrane parametry psychologiczne w zaleznosci od podawanej pacjentowi liczby tabletek.

The main purpose of this study is to estimate the influence of taking a number of placebo tablets to obtain hypotensive effects and some psychological parameters with essential hypertension. 197 patients (102F/95M) aged between 18-80 years (average age 52.3) with essential hypertension were included in the research in two outpatients clinics. The patients who participated in research, stayed for at least seven days without any pharmacological treatment. They had a diastolic pressure (DBP) between 95-114 mm Hg and systolic pressure (SBP) up to 200 mm Hg. 92 patients had taken one tablet a day and 105 patients had taken two tablets in the morning. This was before the placebo was included and two weeks after their blood pressure had been taken by standard methods (sphygmomanometer). They were monitored daily throughout the period using traditional methods and ABPM (SpaceLabs 90121). The patients completed two psychological tests. 97 records (52F/45M) qualified for statistical analysis (80% of measurements were undertaken properly, and the time between measurements was less than two hours). Psychological questionnaires were correctly completed by 92 patients. The visible results of decreasing systolic and diastolic value of blood pressure were obtained after using one (group 1) and two (group 2) placebo tablets. They were monitored by a standard method and recorded by ABPM. In group 1 SBP decreased from 164 +/- 11.4 mm Hg to 158 +/- 8.8 mm Hg (p < 0.01, on standard method) and from 149.5 +/- 17.8 to 144.5 +/- 8.3 mm Hg daily (p < 0.05, ABPM). DBP in this examined group decreased from 106.2 +/- 2.8 to 102 +/- 3.9 mm Hg (p < 0.01, stand. method), and from 103.2 +/- 2.6 to 98.6 +/- 1.2 mm Hg daily (p < 0.05, ABPM). In the second group SBP decreased from 169 +/- 12.8 to 157.6 +/- 17.9 mm Hg (p < 0.001, stand. method), and in ABPM from 148.5 +/- 15.8 to 139.6 +/- 16.2 mm Hg (p < 0.01). In this same group, DBP decreased from 104.4 +/- 2.6 to 98 +/- 3.4 mm hG (p < 0.001, stand. method), and from 107.4 +/- 5.8 mm Hg to 95.5 +/- 4.2 mm Hg-daily (p < 0.001, ABPM). There were no differences between heart rate in all groups of patients. In both groups there were significant statistical differences in negative symptoms, physical symptoms and anexity, but a more optimistic mood was observed by group 2. More effective hypertensive treatment was observed within the group which was treated with two placebo tablets. This group was characterized by more optimistic mood compared to the group which was treated with one placebo tablet a day.