ABSTRACT
The local treatment effects of EGF forms on alkali burned mice corneal wounds were identified. The corneal wounds were induced by 0.5 M NaOH solution on the corneal surfaces of the mice. The local epidermal growth factor solutions (100 ng/ml) and gel form in 0.2 per cent w/w carbopol 940 (100 ng/ml) were dropped in 5 microliters aliquots into the eye twice a day. The corneal wounds were measured for 15 days at 7-day intervals and examined histologically at the end of 15th day of the experimental period. The results indicated that topical epidermal growth factor treatment in solution improved the healing of alkali burned corneal wounds when compared with epidermal growth factor delivered in a polymer system.
Subject(s)
Burns, Chemical/drug therapy , Epidermal Growth Factor/therapeutic use , Eye Burns/drug therapy , Wound Healing/drug effects , Administration, Topical , Animals , Cornea/drug effects , Cornea/pathology , Drug Carriers , Epidermal Growth Factor/administration & dosage , Eye Burns/chemically induced , Female , Male , Mice , Mice, Inbred C57BL , Mice, Inbred Strains , SolutionsABSTRACT
The effect of topically administered epidermal growth factor (EGF) dosage forms was investigated on skin wound healing in mice. Two EGF dosage forms were prepared containing 100 ng mL-1 EGF. The solution dosage form was prepared in 0.9% w/v NaCl. A bioadhesive gel form was prepared in 0.2% Carbopol 940 polymer. The two dosage forms were applied on the skin incision wounds of mice at the rate of 5 microL twice a day for 7 and 15 days. The wound tear strength was tested for skin wound healing at the 7th and 15th days of treatment and compared with controls. The results indicate that the wound tear strength of mice were significantly higher at the 15th day of treatment in the gel-treated group compared with the solution-treated mice and controls (P < 0.001).
Subject(s)
Epidermal Growth Factor/administration & dosage , Epidermal Growth Factor/pharmacology , Skin/drug effects , Wound Healing/drug effects , Administration, Cutaneous , Animals , Female , Male , Mice , Skin/injuriesABSTRACT
We have investigated the generation of a prooxidant state and effect on prostaglandin E (PGE) levels after administration of diethylnitrosamine (DENA) to rats. Lipid peroxides were assayed by monitoring thiobarbituric acid (TBA) a reactive material. PGE--like activity was assayed by using bioassay procedure. Our results demonstrate that increased lipid peroxide and decreased PGE levels were found in a dose dependent manner in DENA injected groups when compared to control groups. According to these findings, it is suggested that there is a negative correlation between carcinogenic DENA induced lipid peroxidation and PGE in rat's liver, lung, and renal tissue.
Subject(s)
Diethylnitrosamine/pharmacology , Dinoprostone/metabolism , Kidney/drug effects , Lipid Peroxidation/drug effects , Liver/drug effects , Lung/drug effects , Animals , Female , Gastric Fundus/drug effects , Kidney/metabolism , Liver/metabolism , Lung/metabolism , Male , Rats , Reactive Oxygen Species , Thiobarbituric Acid Reactive Substances/analysisABSTRACT
Acute exercise may induce free-radical production in mitochondria during basal metabolism of aerobic cells. Ascorbic acid is a strong antioxidant agent, whereas myoglobin is known to act as an oxygen reservoir. The purpose of this study was to investigate the effects of acute exercise on malondialdehyde levels in gastrocnemius, vastus medialis, and triceps brachi muscles and erythrocytes. In addition, we investigated the ascorbic acid levels and serum myoglobin concentrations in rats, following acute swimming exercise. We found that the levels of muscle malondialdehyde and serum myoglobin increased and the levels of plasma ascorbic acid decreased, in proportion to the duration of exercise; however, the levels of erythrocyte malondialdehyde did not change.
Subject(s)
Ascorbic Acid/blood , Erythrocytes/metabolism , Malondialdehyde/metabolism , Muscles/metabolism , Myoglobin/blood , Passive Cutaneous Anaphylaxis/physiology , Animals , Lipid Peroxidation/physiology , Male , Rats , Rats, Wistar , SwimmingABSTRACT
We identified the local treatment effect of epidermal growth factor on corneal wound healing of alloxan diabetic mice. The corneal wounds were induced by 0.5 M NaOH solution on the corneal surfaces of the diabetic and non-diabetic groups. The local epidermal growth factor solution (100 ng ml-1) was dropped in 5-microliters aliquots into the eye twice a day. The corneal wounds were measured daily for 15 days and examined histologically at the end of 15th day of experimental period. The results indicated that topical epidermal growth factor treatment of diabetic and non-diabetic mice greatly improved the incidence of healing of wounded corneas.
