ABSTRACT
AIM: To study relationship between Yersinia pseudotuberculosis cultivation temperature and immunobiological properties of lypopolysaccharide (LPS). MATERIALS AND METHODS: LPS producing strain--Yersinia pseudotuberculosis 164/84 OX; experimental animal--guinea pigs, rabbits; methods--immunochemical (indirect hemagglutionation assay, RDP), physico-chemical (electrophoresis, gas-liquid chromatography), standard biochemical and statistical methods. RESULTS: It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10 degrees C to 37 degrees C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amount of electrophoretically detected O-side chains. It was assumed that the polysaccharide component plays its role in LPS toxicity. CONCLUSION: Relation between Yersinia pseudotuberculosis cultivation temperature and structural and functional properties of LPS preparations obtained from the culture was determined.
Subject(s)
Lipid A/immunology , Yersinia pseudotuberculosis/growth & development , Yersinia pseudotuberculosis/immunology , Acylation , Alcohol Oxidoreductases/metabolism , Animals , Chemical Precipitation , Guinea Pigs , Immune Sera/immunology , Lethal Dose 50 , Lipid A/chemistry , Lipid A/toxicity , Rabbits , TemperatureABSTRACT
The choice of efficient antibacterial drugs useful in special prophylaxis and treatment of plague includes the stage of the in vivo screening whose main disadvantages are long-term and hazardous tests. The paper presents the data showing that a useful chemotherapeutic for the treatment of plague could be safely screened within 48-72 hours. The methods are based on two-fold retrobulbar or intraperitoneal administration of a drug within 24 hours to albino mice infected with highly virulent strains of Y. pestis. The dose should not exceed 1.10(7) live microbes. It was shown that the administration of the plague microbes to albino mice simultaneously with ferrous sulfate lowered the time of the animal death and accelerated the efficacy estimation of the drug.
