ABSTRACT
The purpose of this research was an assessment of neuroprotective effect of ACTH (4-9) in degenerative changes of nerve cells induced by dexamethasone. Experiments were led on Albino-Swiss mouse males. We examine morphological changes of neurons in the dorsal hippocampus in slides stained with cresyl violet and we performed quantitative analysis of neurodegenerative changes using a computer analyser of histological pictures. Achieved results indicate that ACTH (4-9) shows neuroprotective effect against neurotoxic influence of dexamethasone. This chemical inhibits dexamethasone induced degeneration of hippocampal nerve cells having morphological features characteristic of apoptosis.
Subject(s)
Adrenocorticotropic Hormone/pharmacology , Body Weight/drug effects , Dexamethasone/administration & dosage , Nerve Degeneration/pathology , Peptide Fragments/pharmacology , Pyramidal Cells/drug effects , Pyramidal Cells/pathology , Animals , Apoptosis/drug effects , Cell Count , Dexamethasone/toxicity , Dose-Response Relationship, Drug , Drug Interactions , Hippocampus/drug effects , Hippocampus/pathology , Injections, Intraperitoneal , Male , Mice , Nerve Degeneration/chemically induced , Neuroprotective Agents/pharmacology , Reference ValuesABSTRACT
The Loeventhal gland of the white Wistar rats was examined. The animals were given Metizol for 21 days and 42 days at the dose of 1 mg/kg b.m./24 h. The Loeventhal gland's samples were taken for histological and histochemical examination. Then they there stained with hematoxylin and eosin, Masson's, PAS's, and Feulgen's method. The mean area of section of cell nuclei was measured. Results of examination were counted statistically. The following changes were noticed: after 21 days of administration of Metizol in the Loeventhal gland the mean area of the section of cells nuclei was decreased; after 42 days of administration of Metizol the mean area of the section of cell nuclei was decreased as well, but to a lesser degree than in group 1. New follicles appeared which can be the expression of cell mitotic activity.
