Subject(s)
Adjuvants, Immunologic/pharmacology , Carbon Tetrachloride Poisoning/immunology , Spleen/immunology , Adjuvants, Immunologic/isolation & purification , Animals , Antibody Formation/drug effects , Antibody-Producing Cells/drug effects , Antibody-Producing Cells/immunology , Hypersensitivity, Delayed/immunology , Immunization , Methods , Molecular Weight , Rats , Rosette FormationABSTRACT
Immunochemically pure IgE was obtained from the serum of LOW rats carrying IgE-plasmocytome by the immunochemical methods (ion-exchange chromatography and gel filtration). This IgE served as an antigen for immunization of a rabbit to produce a specific antiserum. The monospecificity of this antiserum was achieved by its sorption with normal serum proteins.
Subject(s)
Antibody Specificity , Immune Sera/isolation & purification , Immunoglobulin E/immunology , Animals , Electrophoresis, Polyacrylamide Gel , Immunization/methods , Immunoglobulin E/isolation & purification , Neoplasms, Experimental/immunology , Passive Cutaneous Anaphylaxis , Plasmacytoma/immunology , Rabbits , Rats , Rats, Inbred Strains , Time FactorsABSTRACT
The modified method for obtaining encephalitogenic protein from the brain of a guinea pig with the use of gel filtration and ion-exchange chromatography is described. The electrophoretic mobility of the isolated protein is characterized and its molecular weight is determined.
Subject(s)
Brain Chemistry , Myelin Proteins/isolation & purification , Animals , Chromatography, Gel/methods , Chromatography, Ion Exchange/methods , Electrophoresis, Polyacrylamide Gel/methods , Guinea PigsABSTRACT
Action of Con-A on the proliferative activity of spleen lymphocytes was studied using germfree and conventional Fisher rats 3-4 months of age and conventional 2-3 day old rats. The intensity of 3H-thymidine incorporation in lymphocytes of germfree and conventional animals was the same, however, the coefficient of stimulation was higher in germfree rats. Lymphocytes of suckling rats had a low reactivity to Con-A.