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3.
Zh Mikrobiol Epidemiol Immunobiol ; (4): 109-12, 1983 Apr.
Article in Russian | MEDLINE | ID: mdl-6868884

ABSTRACT

Immunochemically pure IgE was obtained from the serum of LOW rats carrying IgE-plasmocytome by the immunochemical methods (ion-exchange chromatography and gel filtration). This IgE served as an antigen for immunization of a rabbit to produce a specific antiserum. The monospecificity of this antiserum was achieved by its sorption with normal serum proteins.


Subject(s)
Antibody Specificity , Immune Sera/isolation & purification , Immunoglobulin E/immunology , Animals , Electrophoresis, Polyacrylamide Gel , Immunization/methods , Immunoglobulin E/isolation & purification , Neoplasms, Experimental/immunology , Passive Cutaneous Anaphylaxis , Plasmacytoma/immunology , Rabbits , Rats , Rats, Inbred Strains , Time Factors
4.
Article in Russian | MEDLINE | ID: mdl-7124211

ABSTRACT

The modified method for obtaining encephalitogenic protein from the brain of a guinea pig with the use of gel filtration and ion-exchange chromatography is described. The electrophoretic mobility of the isolated protein is characterized and its molecular weight is determined.


Subject(s)
Brain Chemistry , Myelin Proteins/isolation & purification , Animals , Chromatography, Gel/methods , Chromatography, Ion Exchange/methods , Electrophoresis, Polyacrylamide Gel/methods , Guinea Pigs
5.
Tsitologiia ; 22(11): 1365-8, 1980 Nov.
Article in Russian | MEDLINE | ID: mdl-7445093

ABSTRACT

Action of Con-A on the proliferative activity of spleen lymphocytes was studied using germfree and conventional Fisher rats 3-4 months of age and conventional 2-3 day old rats. The intensity of 3H-thymidine incorporation in lymphocytes of germfree and conventional animals was the same, however, the coefficient of stimulation was higher in germfree rats. Lymphocytes of suckling rats had a low reactivity to Con-A.


Subject(s)
Concanavalin A/pharmacology , Germ-Free Life/drug effects , Lymphocytes/drug effects , Spleen/cytology , Animals , Animals, Newborn , Cell Division/drug effects , Female , Lymphocyte Activation/drug effects , Male , Rats
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