ABSTRACT
Abstract The study of biologically active substances-secondary metabolites of plants that exhibit geroprotective properties is an actual and popular direction in medicine to prevent early aging. This work aims to select the cultivation parameters for obtaining in vitro cell cultures of meadowsweet containing the largest amount of biologically active substances (BAS) for their further extraction as candidate substances for geroprotectors. To specify the effectiveness of the selected cell culture cultivation parameters, biomass growth for callus and root cultures, growth index, specific growth rate, and viability for suspension cultures was carried out. The study results made it possible to select the nutrient media for the cultivation of cell cultures of meadowsweet. It has been found that the greater the antioxidant activity of the extracts, the greater the antimicrobial properties it exhibits. In this study, cell cultures in vitro and alcohol extracts from the plant Filipendula ulmaria were considered as raw materials rich in candidate substances for geroprotectors. According to the data obtained, the plant is rich in hydroxybenzoic and salicylic acids, spireoside, avicularin, and hyperoside.
Resumo O estudo de substâncias biologicamente ativas metabólitos secundários de plantas que apresentam propriedades geroprotetoras é uma tendência atual e popular no campo da medicina para a prevenção do envelhecimento precoce. O objetivo deste trabalho foi selecionar os parâmetros de cultivo para obtenção de culturas celulares in vitro de Ulmária contendo a maior quantidade de substâncias biologicamente ativas (SBA), para sua posterior extração como substâncias candidatas a serem geroprotetoras. Para especificar a eficácia dos parâmetros selecionados de cultivo em cultura de células, foi realizada a análise de crescimento de biomassa para culturas de calos e raízes, índice de crescimento, taxa de crescimento específica e viabilidade para culturas em suspensão. Os resultados do estudo possibilitaram a seleção do meio nutriente para o cultivo de células de Ulmária. Verificou-se que, quanto maior a atividade antioxidante dos extratos, maiores eram as propriedades antimicrobianas exibidas. Neste estudo, culturas celulares in vitro e extratos alcoólicos da planta Filipendula ulmaria foram considerados matérias-primas ricas em substâncias candidatas a serem geroprotetoras. De acordo com os dados obtidos, a planta é rica em ácidos hidroxibenzoico e salicílico, espirosídeo, avicularina e hiperosídeo.
ABSTRACT
The study of biologically active substances-secondary metabolites of plants that exhibit geroprotective properties is an actual and popular direction in medicine to prevent early aging. This work aims to select the cultivation parameters for obtaining in vitro cell cultures of meadowsweet containing the largest amount of biologically active substances (BAS) for their further extraction as candidate substances for geroprotectors. To specify the effectiveness of the selected cell culture cultivation parameters, biomass growth for callus and root cultures, growth index, specific growth rate, and viability for suspension cultures was carried out. The study results made it possible to select the nutrient media for the cultivation of cell cultures of meadowsweet. It has been found that the greater the antioxidant activity of the extracts, the greater the antimicrobial properties it exhibits. In this study, cell cultures in vitro and alcohol extracts from the plant Filipendula ulmaria were considered as raw materials rich in candidate substances for geroprotectors. According to the data obtained, the plant is rich in hydroxybenzoic and salicylic acids, spireoside, avicularin, and hyperoside.
O estudo de substâncias biologicamente ativas - metabólitos secundários de plantas que apresentam propriedades geroprotetoras - é uma tendência atual e popular no campo da medicina para a prevenção do envelhecimento precoce. O objetivo deste trabalho foi selecionar os parâmetros de cultivo para obtenção de culturas celulares in vitro de Ulmária contendo a maior quantidade de substâncias biologicamente ativas (SBA), para sua posterior extração como substâncias candidatas a serem geroprotetoras. Para especificar a eficácia dos parâmetros selecionados de cultivo em cultura de células, foi realizada a análise de crescimento de biomassa para culturas de calos e raízes, índice de crescimento, taxa de crescimento específica e viabilidade para culturas em suspensão. Os resultados do estudo possibilitaram a seleção do meio nutriente para o cultivo de células de Ulmária. Verificou-se que, quanto maior a atividade antioxidante dos extratos, maiores eram as propriedades antimicrobianas exibidas. Neste estudo, culturas celulares in vitro e extratos alcoólicos da planta Filipendula ulmaria foram considerados matérias-primas ricas em substâncias candidatas a serem geroprotetoras. De acordo com os dados obtidos, a planta é rica em ácidos hidroxibenzoico e salicílico, espirosídeo, avicularina e hiperosídeo.
Subject(s)
Plants, Medicinal/genetics , Aging , Aging, Premature , AntioxidantsABSTRACT
Studies on birth rate regularities in Russia in the context of the task of ensuring reproduction of the population remain relevant. Assessment of the scale and trends in regional differentiation using the method of calculating birth rates in real generations of women makes it possible to obtain a more reliable picture of the ongoing processes, to assess the reproductive potential and target groups for the most effective implementation of demographic policy measures. The article presents results of calculations of fertility rates in real generations of women for all regions of the Russian Federation in line with the 2020 population census. The analysis has identified significant differences in the scale and patterns of fertility in the generations of women in the Russian regions. In 34 constituent entities of the Russian Federation, the difference (in one direction or another) between places in rows in terms of the average number of children born to women aged 40-44 (according to the 2020 population census) and the total fertility rate (2021 data) exceeds 10 places. The potential is best realized in the national republics, while in the regions of Central Russia small families prevail. The prognostic possibilities of using the expected numbers of children are proved, and the use of the total fertility rate indicator as a target for the Russian average indicators was validated. Interregional differentiation is significantly higher for the third birth, which is confirmed by the calculated coefficient of variation.
Subject(s)
Birth Rate , Censuses , Female , Humans , Russia/epidemiology , AdultABSTRACT
The study of biologically active substances-secondary metabolites of plants that exhibit geroprotective properties is an actual and popular direction in medicine to prevent early aging. This work aims to select the cultivation parameters for obtaining in vitro cell cultures of meadowsweet containing the largest amount of biologically active substances (BAS) for their further extraction as candidate substances for geroprotectors. To specify the effectiveness of the selected cell culture cultivation parameters, biomass growth for callus and root cultures, growth index, specific growth rate, and viability for suspension cultures was carried out. The study results made it possible to select the nutrient media for the cultivation of cell cultures of meadowsweet. It has been found that the greater the antioxidant activity of the extracts, the greater the antimicrobial properties it exhibits. In this study, cell cultures in vitro and alcohol extracts from the plant Filipendula ulmaria were considered as raw materials rich in candidate substances for geroprotectors. According to the data obtained, the plant is rich in hydroxybenzoic and salicylic acids, spireoside, avicularin, and hyperoside.
Subject(s)
Filipendula , Antioxidants , Plant Extracts/pharmacologyABSTRACT
The iron-containing protein neuroglobin (Ngb) involved in the transport of oxygen is generally considered the precursor of all animal globins. In this report, we studied the structure of Ngb of the cold-water sponge Halisarca dujardinii. In sponges, the oldest multicellular organisms, the Ngb gene contains three introns. In contrast to human Ngb, its promoter contains a TATA-box, rather than CG-rich motifs. In sponges, Ngb consists of 169 amino acids showing rather low similarity with its mammalian orthologues. It lacks Glu and Arg residues in positions required for prevention of hypoxia-related apoptosis. Nevertheless, Ngb contains both proximal and distal conserved heme-biding histidines. The primary structure of H. dujardinii neuroglobin predicted by sequencing was confirmed by mass-spectrometry analysis of recombinant Ngb expressed in E. coli. The high level of Ngb expression in sponge tissues suggests its possible involvement in the gas metabolism and presumably in other key metabolic processes in H. dujardinii.
Subject(s)
Neuroglobin/chemistry , Porifera/chemistry , Amino Acids , Animals , Escherichia coli , Introns , Promoter Regions, GeneticABSTRACT
The International Committee for Weights and Measures (CIPM), at its meeting in October 2017, followed the recommendation of the Consultative Committee for Units (CCU) on the redefinition of the kilogram, ampere, kelvin and mole. For the redefinition of the kelvin, the Boltzmann constant will be fixed with the numerical value 1.380 649 × 10-23 J K-1. The relative standard uncertainty to be transferred to the thermodynamic temperature value of the triple point of water will be 3.7 × 10-7, corresponding to an uncertainty in temperature of 0.10 mK, sufficiently low for all practical purposes. With the redefinition of the kelvin, the broad research activities of the temperature community on the determination of the Boltzmann constant have been very successfully completed. In the following, a review of the determinations of the Boltzmann constant k, important for the new definition of the kelvin and performed in the last decade, is given.
ABSTRACT
The mise-en-pratique for the definition of the kelvin at high temperatures will formally allow dissemination of thermodynamic temperature either directly or mediated through high-temperature fixed points (HTFPs). In this paper, these two distinct dissemination methods are evaluated, namely source-based and detector-based. This was achieved by performing two distinct dissemination trials: one based on HTFPs, the other based on absolutely calibrated radiation thermometers or filter radiometers. These trials involved six national metrology institutes in Europe in the frame of the European Metrology Research Programme joint project 'Implementing the new kelvin' (InK). The results have shown that both dissemination routes are possible, with similar standard uncertainties of 1-2 K, over the range 1273-2773 K, showing that, depending on the facilities available in the laboratory, it will soon be possible to disseminate thermodynamic temperatures above 1273 K to users by either of the two methods with uncertainties comparable to the current temperature scale.
ABSTRACT
We describe a high-performance, transportable, versatile spectroradiometer based on an acousto-optical tunable filter (AOTF). The instrument was developed for temperature metrology, namely, to determine the thermodynamic temperature of black bodies above the Ag freezing point (961.78 °C). Its main design feature is the attenuation of the diffraction side lobes (and, thus, out-of-band stray light) thanks to the use of a double-pass configuration. The radiofrequency tuning of the AOTF allows continuous, fine, and rapid wavelength control over a wide spectral range (650 nm-1000 nm). The instrument tunability can be easily calibrated with an Ar spectral lamp with reproducibility within 10 pm over one week. The instrument was characterised in terms of relative signal stability (few 10-4) and wavelength stability (1 pm) over several hours. The spectral responsivity of the instrument was calibrated with two complementary methods: tuning of the wavelength of the optical source or tuning the radiofrequency of the AOTF. Besides the application for thermodynamic temperature determination at the lowest uncertainty level, this instrument can also be used for multispectral non-contact thermometry of processed materials of non-grey and non-unitary emissivity (in the glass or metallurgical industries).
ABSTRACT
In the work there was performed an assessment of the interaction of microsocial and genetic factors of the development of psychoactive substance (PS) dependence. The objects of the psycho-hygienic and molecular-genetic studies were 538 male patients from the specialized diagnostic and treatment center at the age from 17 to 65 years with a diagnosis of "PS dependence" according to F10-F09 in the ICD-10. There were determined personality predictors of early (before 25 years) manifestation of systematic abuse, such as low self-control, individualisticity, authoritarianism, unjustified optimism and reduced capacity for social adaptation. Manifestation of the PS dependence at an early age (25 years) is determined by the contribution of genotype 9R+ DAT gene in the combination with other predisposing genotypes A1 + DRD2/ANKK1, SS SERT and 7R+ DRD. The risk of development of PS dependence at a more younger age increases with the superimposition of individual predisposing genotypes ranging from 1,2 (7R+ gene DRD4) to 1,9 (A1 + gene DRD2/ANKK10 on a destructive milieu. Pairwise combinations of genotypes 7R+ DRD4 x A1+ DRD2, 7R+ DRD4 x 9R+ DAT, 9R+ DAT x A1+ DRD2, 9R+ DAT x SS SERT significantly increase the risk by 2 or more times (2.5-2.8). There was suggested an algorithm for the prenosological forecast of the development of PS dependence in adolescents and young men.
Subject(s)
Genetic Predisposition to Disease , Primary Prevention/methods , Psychotropic Drugs/administration & dosage , Substance-Related Disorders/prevention & control , Adolescent , Adult , Age Factors , Age of Onset , Aged , Algorithms , Genotype , Humans , Male , Middle Aged , Molecular Biology , Psychotropic Drugs/adverse effects , Risk Factors , Substance-Related Disorders/rehabilitation , Young AdultABSTRACT
The objective of the present work was to study age-specific histomorphological changes developing in the kidneys in the course of ageing with special reference to the determination of the number of renal glomeruli in the field of vision during the microscopic examination of histological preparations. The study revealed the significant difference between the number of the renal glomeruli in the subjects under and above 50 year old. The results of the work were used to develop the method for the quantitative evaluation of the involutional changes in the kidneys for the determination of the human biological age in the combination with other approaches.
Subject(s)
Aging/pathology , Forensic Pathology/methods , Kidney/pathology , Postmortem Changes , Adult , Aged , Aged, 80 and over , Cadaver , Female , Humans , Male , Microscopy , Middle Aged , Young AdultABSTRACT
Growth patterns and intracellular Ca2+ concentrations in the mutant strain Aspergillus awamori 66A, containing a recombinant aequorin gene were studied in the presence of a permeabilizing fungicidal agent amphotericin B. The cell response, i.e., changes in the growth and development of the fungus (initiation of spore germination, mycelial growth, and intensity ofsporulation) was dose-dependent. Low concentrations of amphotericin B (2.5 microM) stimulated spore germination: the number of germinating spores was 2-3 times higher than in the control (without the fungicide). At higher amphotericin concentrations (20 microM) spore germination was inhibited. Amphotericin B had a dose-dependent effect on mycelial growth and sporulation intensity on solid Vogel medium. Intracellular Ca2+ concentrations in the presence of amphotericin B were investigated using the luminescence of the photoprotein aequorin. High concentrations of amphotericin B (10 and 20 microM) were shown to cause an instantaneous increase in Ca2+ concentrations, compared to the control and lower amphotericin concentration (2.5 microM). Ca2+ concentrations remained elevated throughout the experiment and correlated with the inhibition of mycelial growth and development.
Subject(s)
Amphotericin B/pharmacology , Antifungal Agents/pharmacology , Aspergillus/physiology , Calcium/metabolism , Mutation , Spores, Fungal/metabolism , Dose-Response Relationship, Drug , Spores, Fungal/geneticsABSTRACT
In the paper there are presented the results of a study of anxiety and balance ofparts of autonomous nervous system in healthy children 5-7 years old, residing in different parts of Magnitogorsk. It is shown that state of heightened and high alert was shown to be more common among children living on the left bank of the Urals river around the Magnitogorsk Metallurgical Integrated Plant. In these children an imbalance in the work of the parts of the autonomic nervous system was detected more frequently, at that shifts were observed mainly in the direction to ergotropic tone. At the same time balanced work of the parts of the autonomic nervous system was observed more frequently in children living on the right bank of the Urals river. Discovered psychosomatic features of examined children turned out to be associated with both the social characteristics of family lifestyle and the emotional stress of parents, and the contents of some organic compounds in total snow samples collected in the territories of kindergartens which they attended. One ofthe most significant results ofthe work we consider the detection of a correlation relationship between emotional stress of parents and activity of key enzymes in their children, reflecting the protective and adaptive reactions of the organism. On the basis of these and previously obtained data, we suggest that social and psychological factors of the family are not only a potential source of maladaptation of the child, but, probably, can have an impact on the stability and sensitivity of the genome of children.
Subject(s)
Air Pollutants , Anxiety/psychology , Autonomic Nervous System/physiology , Metallurgy , Adaptation, Physiological , Adaptation, Psychological , Air Pollutants/adverse effects , Air Pollutants/analysis , Anxiety/genetics , Anxiety/physiopathology , Autonomic Nervous System/drug effects , Case-Control Studies , Child , Child, Preschool , Environmental Monitoring , Genomic Instability , Humans , Russia , Socioeconomic FactorsABSTRACT
The results of an annual prophylactic medical examination were studied in 288 women aged 60-87 years. Primary and repeated visits revealed that 92.9% of the patients had endometrial pathology presented with glandular-fibrous polyps that both recurred and occurred de novo. Adenocarcinomas developing 4-14 years after polypectomy were diagnosed in 7.1% of the women. Focal impairments in the differentiation of an epithelial component of endometrial polyps in their recurrences and repeated development were noted in 97.3% of cases. In 88% of the patients, complaints were absent and recurrent endometrial pathology, including one of three adenocarcinomas, were diagnosed by prophylactic medical examination. The false-positive diagnoses of endometrial pathology by ultrasound study were 5.2%. Hysteroscopy in combination with morphology study should be recognized to be the gold standard for diagnosing pathologic endometrial lesions.
Subject(s)
Endometrium/diagnostic imaging , Neoplasm Recurrence, Local/diagnostic imaging , Polyps/diagnostic imaging , Uterine Neoplasms/diagnostic imaging , Adenocarcinoma/diagnostic imaging , Adenocarcinoma/pathology , Adenocarcinoma/surgery , Aged , Aged, 80 and over , Endometrial Neoplasms/diagnostic imaging , Endometrial Neoplasms/pathology , Endometrial Neoplasms/surgery , Endometrium/pathology , Female , Humans , Middle Aged , Neoplasm Recurrence, Local/pathology , Polyps/pathology , Polyps/surgery , Ultrasonography , Uterine Neoplasms/pathologyABSTRACT
To avoid serious causes of dizziness and establishing preliminary diagnosis is usually enough sighting history taking and physical examination of the patient. Great importance is self-presenting patients with complaints of dizziness. In recent years, for treatment of various types vestybulopatiy, but also for the prevention of attacks, successfully used betahistyn hydrochloride, which improves the quality of care and well-being of the patient.
Subject(s)
Dizziness/diagnosis , Vertigo/diagnosis , Vestibular Neuronitis/diagnosis , Adult , Betahistine/administration & dosage , Betahistine/therapeutic use , Diagnosis, Differential , Dizziness/drug therapy , Dizziness/etiology , Female , Histamine Agonists/administration & dosage , Histamine Agonists/therapeutic use , Humans , Male , Treatment Outcome , Vertigo/drug therapy , Vertigo/etiology , Vestibular Neuronitis/drug therapy , Vestibular Neuronitis/etiology , Young AdultABSTRACT
The paper presents the results of investigating the phenotypic polymorphism of a number of biochemical and immunological parameters (the values of oxidative stress, the activity of catalase and glutathione-S-transferase in red blood cells, the serum levels of catecholamines, tumor-necrosis factor-?, and IgG antibody subclasses) in the authors' hygienic studies of genotypic and nongenotypic population samples.
Subject(s)
Health Status Indicators , Hygiene , Oxidative Stress , Polymorphism, Genetic , Biomarkers/blood , Catalase/blood , Catalase/genetics , Catecholamines/blood , Environmental Exposure/analysis , Environmental Exposure/statistics & numerical data , Erythrocytes/enzymology , Genotype , Glutathione Transferase/blood , Glutathione Transferase/genetics , Humans , Moscow , Oxidative Stress/genetics , Phenotype , Tumor Necrosis Factor-alpha/blood , Tumor Necrosis Factor-alpha/geneticsABSTRACT
The paper summarizes the results of 8 genetic and psychological examinations in adults and children, which are aimed at evaluating the genotoxic effects of environmental factors on cultured lymphocytes. Human emotional tension was proved to be directly related to the level of genome damage, to genomic sensitivity to in vitro exposures, and to lymphocyte proliferative activity and to be inversely related to the level of apoptosis. There was an association of the degree of anxiety in children with their morbidity. High emotional tension in adult family members was found to negatively affect the degree of anxiety and the level of genomic instability in a child.
Subject(s)
Chromosome Aberrations/chemically induced , Environmental Pollutants/toxicity , Genomic Instability/drug effects , Stress, Psychological/genetics , Adult , Apoptosis/drug effects , Cell Proliferation/drug effects , Child , Child, Preschool , Chromosome Aberrations/statistics & numerical data , Humans , Lymphocytes/drug effects , Lymphocytes/ultrastructure , Micronuclei, Chromosome-Defective/chemically induced , Micronuclei, Chromosome-Defective/statistics & numerical data , Russia/epidemiology , Stress, Psychological/blood , Stress, Psychological/epidemiologyABSTRACT
With the help of immune-enzyme analysis the amount of soy protein was determined in cooked meats. The results obtained are subjected to mathematical processing, also shows the metrological performance of the method.
Subject(s)
Food Analysis/methods , Meat Products/analysis , Soybean Proteins/analysis , Enzyme-Linked Immunosorbent Assay/methods , Sensitivity and SpecificityABSTRACT
Steroidal saponins are bioactive substances of Tribulus terrestris and can be used to assess the quality of raw materials and processed products from them. For this purpose has been developed the method of qualitative and quantitative determination of steroidal saponins by high performance liquid chromatography with spectrophotometric and mass-selective detection and optimal conditions of sample preparation (70% methanol extraction with sonication and heating); also has been studied steroidal saponins composition of Tribulus terrestris (protodioscin, tribulosaponin B, metilprotodiostsin, terrestrozin H, prototribestin, gracillin and others were found).
Subject(s)
Dietary Supplements/analysis , Saponins/analysis , Steroids/analysis , Tribulus/chemistry , Chromatography, High Pressure Liquid/methods , Mass Spectrometry , Plant Components, Aerial/chemistry , Plant Extracts/analysis , Plant Extracts/chemistry , Plants, Medicinal/chemistry , Saponins/chemistry , Steroids/chemistryABSTRACT
The paper gives the results of a study of the impact of dopamine (DRD2) and serotonin (5HTR2A) genes on the development of personality characteristics in adolescents, by applying the Cattell (16PF) questionnaire. The study was performed in a group of 360 Moscow teenagers (185 girls and 175 boys) aged 14-17 years. The boys carrying the A1 allelle of the DRD2 gene were found to have a lower self-control, indiscipline, and impulsiveness. An association between the indicators of unconscientiousness, social introversion, and group independence was established in the girls with the G/G genotype of the 5HTR2A gene. Thus, gender differences have been revealed from the impact of dopamine and serotonin gene polymorphisms on the teenagers' personality characteristics that characterize the forms of disadaptive behavior, such as unconscientiousness, indiscipline, low self-control, and impulsiveness.
