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1.
Sensors (Basel) ; 23(19)2023 Oct 05.
Article in English | MEDLINE | ID: mdl-37837078

ABSTRACT

In this paper, a procedure for obtaining undistorted high derivatives (up to the eighth order) of the optical absorption spectra of biomolecule pigments has been developed. To assess the effectiveness of the procedure, the theoretical spectra of bacteriochlorophyll a, chlorophyll a, spheroidene, and spheroidenone were simulated by fitting the experimental spectra using the differential evolution algorithm. The experimental spectra were also approximated using sets of Gaussians to calculate the model absorption spectra. Theoretical and model spectra can be differentiated without smoothing (high-frequency noise filtering) to obtain high derivatives. Superimposition of the noise track on the model spectra allows us to obtain test spectra similar to the experimental ones. Comparison of the high derivatives of the model spectra with those of the test spectra allows us to find the optimal parameters of the filter, the application of which leads to minimal differences between the high derivatives of the model and test spectra. For all four studied pigments, it was shown that smoothing the experimental spectra with optimal filters makes it possible to obtain the eighth derivatives of the experimental spectra, which were close to the eighth derivatives of their theoretical spectra.

2.
Nanomaterials (Basel) ; 13(14)2023 Jul 15.
Article in English | MEDLINE | ID: mdl-37513091

ABSTRACT

Powerful emitters of ultraviolet C (UVC) light in the wavelength range of 230-280 nm are necessary for the development of effective and safe optical disinfection technologies, highly sensitive optical spectroscopy and non-line-of-sight optical communication. This review considers UVC emitters with electron-beam pumping of heterostructures with quantum wells in an (Al,Ga)N material system. The important advantages of these emitters are the absence of the critical problem of p-type doping and the possibility of achieving record (up to several tens of watts for peak values) output optical power values in the UVC range. The review consistently considers about a decade of world experience in the implementation of various UV emitters with various types of thermionic, field-emission, and plasma-cathode electron guns (sources) used to excite various designs of active (light-emitting) regions in heterostructures with quantum wells of AlxGa1-xN/AlyGa1-yN (x = 0-0.5, y = 0.6-1), fabricated either by metal-organic chemical vapor deposition or by plasma-activated molecular beam epitaxy. Special attention is paid to the production of heterostructures with multiple quantum wells/two-dimensional (2D) quantum disks of GaN/AlN with a monolayer's (1 ML~0.25 nm) thickness, which ensures a high internal quantum efficiency of radiative recombination in the UVC range, low elastic stresses in heterostructures, and high-output UVC-optical powers.

3.
Opt Lett ; 48(11): 2957-2960, 2023 Jun 01.
Article in English | MEDLINE | ID: mdl-37262253

ABSTRACT

We report on a gain-switched operation of single-crystal Fe2+:ZnSe pumped by a coupled-cavity Q-switched Er3+:ZrF4 fiber laser. With this approach, we use a continuous wave Er-doped ZrF4 fiber laser whose cavity is coupled with the Fe:ZnSe laser cavity. A stable Q switching operation of the Er-doped fiber laser was achieved in this case and, as a result, a gain-switched operation of the Fe:ZnSe laser was obtained. The highest repetition rate was 79 kHz, the pulse duration of the pump Er-doped laser was ∼1.6 µs, and the pulse duration of the Fe:ZnSe was 62 ns. The maximum output power achieved from gain-switched Fe:ZnSe was 63 mW.

4.
Nanomaterials (Basel) ; 13(6)2023 Mar 16.
Article in English | MEDLINE | ID: mdl-36985973

ABSTRACT

This article describes GaN/AlN heterostructures for ultraviolet-C (UVC) emitters with multiple (up to 400 periods) two-dimensional (2D)-quantum disk/quantum well structures with the same GaN nominal thicknesses of 1.5 and 16 ML-thick AlN barrier layers, which were grown by plasma-assisted molecular-beam epitaxy in a wide range of gallium and activated nitrogen flux ratios (Ga/N2*) on c-sapphire substrates. An increase in the Ga/N2* ratio from 1.1 to 2.2 made it possible to change the 2D-topography of the structures due to a transition from the mixed spiral and 2D-nucleation growth to a purely spiral growth. As a result, the emission energy (wavelength) could be varied from 5.21 eV (238 nm) to 4.68 eV (265 nm) owing to the correspondingly increased carrier localization energy. Using electron-beam pumping with a maximum pulse current of 2 A at an electron energy of 12.5 keV, a maximum output optical power of 50 W was achieved for the 265 nm structure, while the structure emitting at 238 nm demonstrated a power of 10 W.

5.
Opt Express ; 30(22): 40347-40356, 2022 Oct 24.
Article in English | MEDLINE | ID: mdl-36298969

ABSTRACT

We demonstrate the first application of a Cr:CdSe laser for highly-sensitive multicomponent intracavity absorption spectroscopy around λ = 3.1-3.4 µm. A detection scheme based on an integrated recording of multiple (∼70) individual Cr:CdSe laser pulses after a single pump-pulse excitation is reported. The sensitivity of our system corresponds to an effective absorption path length of Leff ≈ 850 m. Exemplary measurements of atmospheric H2O and CH4, and additionally introduced gas-phase HCl, C2H4, or C2H6 are presented. The achieved noise-equivalent detection limits are in the ppb range. Possibilities for further sensitivity enhancement by up to a factor of 104 are discussed.

6.
Biochemistry (Mosc) ; 87(8): 742-751, 2022 Aug.
Article in English | MEDLINE | ID: mdl-36171655

ABSTRACT

Proton-translocating Fo×F1-ATPase/synthase that catalyzes synthesis and hydrolysis of ATP is commonly considered to be a reversibly functioning complex. We have previously shown that venturicidin, a specific Fo-directed inhibitor, blocks the synthesis and hydrolysis of ATP with a significant difference in the affinity [Zharova, T. V. and Vinogradov, A. D. (2017) Biochim. Biophys. Acta, 1858, 939-944]. In this paper, we have studied in detail inhibition of Fo×F1-ATPase/synthase by venturicidin in tightly coupled membranes of Paracoccus denitrificans under conditions of membrane potential generation. ATP hydrolysis was followed by the ATP-dependent succinate-supported NAD+ reduction (potential-dependent reverse electron transfer) catalyzed by the respiratory chain complex I. It has been demonstrated that membrane energization did not affect the affinity of Fo×F1-ATPase/synthase for venturicidin. The dependence of the residual ATP synthase activity on the concentration of venturicidin approximated a linear function, whereas the dependence of ATP hydrolysis was sigmoidal: at low inhibitor concentrations venturicidin strongly inhibited ATP synthesis without decrease in the rate of ATP hydrolysis. A model is proposed suggesting that ATP synthesis and ATP hydrolysis are catalyzed by two different forms of Fo×F1.


Subject(s)
Paracoccus denitrificans , Adenosine Triphosphate , Kinetics , NAD , Proton-Translocating ATPases/metabolism , Protons , Succinates , Venturicidins
7.
Nanomaterials (Basel) ; 11(10)2021 Sep 29.
Article in English | MEDLINE | ID: mdl-34684994

ABSTRACT

Monolayer (ML)-scale GaN/AlN multiple quantum well (MQW) structures for electron-beam-pumped ultraviolet (UV) emitters are grown on c-sapphire substrates by using plasma-assisted molecular beam epitaxy under controllable metal-rich conditions, which provides the spiral growth of densely packed atomically smooth hillocks without metal droplets. These structures have ML-stepped terrace-like surface topology in the entire QW thickness range from 0.75-7 ML and absence of stress at the well thickness below 2 ML. Satisfactory quantum confinement and mitigating the quantum-confined Stark effect in the stress-free MQW structures enable one to achieve the relatively bright UV cathodoluminescence with a narrow-line (~15 nm) in the sub-250-nm spectral range. The structures with many QWs (up to 400) exhibit the output optical power of ~1 W at 240 nm, when pumped by a standard thermionic-cathode (LaB6) electron gun at an electron energy of 20 keV and a current of 65 mA. This power is increased up to 11.8 W at an average excitation energy of 5 µJ per pulse, generated by the electron gun with a ferroelectric plasma cathode at an electron-beam energy of 12.5 keV and a current of 450 mA.

8.
Opt Express ; 29(8): 12033-12048, 2021 Apr 12.
Article in English | MEDLINE | ID: mdl-33984972

ABSTRACT

We demonstrate an intracavity absorption spectroscopy system based on a broadband single-crystal pulsed Fe:ZnSe laser. The laser operates at room-temperature and is continuously tunable in the spectral range of 3.76-5.29 µm. The long-wavelength emission up to 5.29 µm is a record achievement for Fe:ZnSe lasers, to the best of our knowledge. The developed laser system is applied for measurements of gaseous absorption inside the laser resonator. We demonstrate sensitive detection of (i) CO2 isotopes in the atmosphere and in human breath, (ii) CO in breath (after cigarette smoking) and in the smoke of a smoldering paper, and (iii) N2O in a gas flow. The achieved detection limits are: 0.1 ppm for 12CO2 and 13CO2, 3 ppm for CO, and 1 ppm for N2O. The sensitivity of the current system is primarily limited by the short pump-pulse duration of 40 ns. Possibilities for sensitivity enhancement by up to a factor of 107 are discussed.

9.
J Phys Chem B ; 125(14): 3538-3545, 2021 04 15.
Article in English | MEDLINE | ID: mdl-33818091

ABSTRACT

The excitation energy transfer (EET) from the bacteriochlorophyll (BChl) Soret band to the second excited state(s) (S2) of carotenoids in pigment-protein complexes of purple bacteria was investigated. The efficiency of EET was determined, based on fluorescence excitation and absorption spectra of chromatophores, peripheral light-harvesting complexes (LH2), core complexes (LH1-RC), and pigments in solution. Carotenoid-containing and carotenoid-less samples were compared: LH1-RC and LH2 from Allochromatium minutissimum, Ectothiorhodospira haloalkaliphila, and chromatophores from Rhodobacter sphaeroides and Rhodospirillum rubrum wild type and carotenoid-free strains R-26 and G9. BChl-to-carotenoid EET was absent, or its efficiency was less than the accuracy of the measurements of ∼5%. Quantum chemical calculations support the experimental results: The transition dipole moments of spatially close carotenoid/BChl pairs were found to be nearly orthogonal. The structural arrangements suggest that Soret EET may be lacking for the studied systems, however, EET from carotenoids to Qx appears to be possible.


Subject(s)
Photosynthetic Reaction Center Complex Proteins , Rhodobacter sphaeroides , Bacteriochlorophylls , Carotenoids , Chromatiaceae , Ectothiorhodospira , Energy Transfer , Light-Harvesting Protein Complexes/metabolism , Photosynthetic Reaction Center Complex Proteins/metabolism , Proteobacteria/metabolism , Rhodobacter sphaeroides/metabolism , Spectrometry, Fluorescence
10.
Opt Express ; 28(12): 17449-17456, 2020 Jun 08.
Article in English | MEDLINE | ID: mdl-32679952

ABSTRACT

We report a laser operation from a Fe:CdTe single crystal, pumped by 40-ns pulses of a 4.12-µm Fe:ZnSe laser. The maximum output energy of 5.8 mJ was produced at 5.4 µm with 30% absorbed energy slope efficiency. A record 2300-nm-wide smooth and continuous wavelength tunability over 4.5-6.8 µm range was demonstrated, being the longest wavelength tuning achieved for Fe2+-doped chalcogenide lasers. We also discuss the features of the oscillation spectra.

11.
Photosynth Res ; 146(1-3): 17-24, 2020 Dec.
Article in English | MEDLINE | ID: mdl-31655967

ABSTRACT

We provide here an edited version of the "Farewell discussion" by the late Aleksandr (Alex) Yuryevich (Yu) Borisov (1930-2019) on several aspects related to the excitation energy transfer in photosynthetic bacteria. It is preceded by a prolog giving the events that led to our decision to publish it. Further, we include here a few photographs to give a personal glimpse of this unique biophysicist of our time. In addition, we provide here a reminiscence, by Andrei B. Rubin, on the scientific beginnings of Borisov. This article follows a Tribute to Borisov by Semenov et al. (2019, Photosynthesis Research, this issue).


Subject(s)
Energy Transfer , Photosynthesis , Proteobacteria/metabolism , Rhodospirillum rubrum/metabolism , Bacteriochlorophylls/history , Bacteriochlorophylls/metabolism , History, 20th Century , History, 21st Century , Photochemistry/history , Photosynthetic Reaction Center Complex Proteins/history , Surveys and Questionnaires
12.
Opt Express ; 27(9): 12090-12099, 2019 Apr 29.
Article in English | MEDLINE | ID: mdl-31052754

ABSTRACT

We report on the study of quenching and thermal lensing based on simple effective lens approximation in a Cr2+:CdSe active medium, including detailed research on the medium's luminescence lifetime dependence on temperature in the 236-391 K range. This work has allowed us to partially overcome the limitations associated with thermal effects in the medium and build a laser system that allowed power scalability to be realized for the Cr2+:CdSe laser. Longitudinal pumping using a continuous-wave Tm-doped fiber laser at 1.908 µm produced an output of 2.3 W at 2.65 µm with an absorbed pump power slope efficiency of 47.6%, which, to the best of our knowledge, is the highest output power achieved in Cr:CdSe continuous-wave lasers.

13.
Opt Lett ; 44(5): 1285-1288, 2019 Mar 01.
Article in English | MEDLINE | ID: mdl-30821769

ABSTRACT

We report the efficient gain-switched high-repetition-rate Cr2+:CdSe single-crystal laser operating around 3 µm and pumped at 2.09 µm by a fiber-laser-pumped Ho3+:YAG laser. Average power of up to 6 W with the optical-to-optical efficiency of 67% at 2.65-2.85 µm was achieved in the Cr2+:CdSe laser with nanosecond pulses at 8 kHz repetition rate. Wavelength tunability from 2.5 µm to 3.15 µm was demonstrated using a set of cavity mirrors and an intracavity Lyot filter.

14.
Biochim Biophys Acta Bioenerg ; 1858(2): 109-117, 2017 Feb.
Article in English | MEDLINE | ID: mdl-27810396

ABSTRACT

Bovine heart mitochondrial respiratory complex II generates ROS, mostly as superoxide, at the rate of about 20% of that detected during simultaneous operation of complex I and II when oxidation of ubiquinol is prevented by myxothiazol. ROS generating activity at different fumarate/succinate concentrations ratio implies that an enzyme component with a midpoint potential 40mV more positive than that of fumarate/succinate couple is the donor for one-electron reduction of oxygen. This suggests that the iron-sulfur cluster(s) is(are) involved in superoxide formation. Complex II-mediated ROS production exhibits a maximum at low (about 50µM) succinate concentration and gradually declines to zero activity upon further increase of the substrate. This phenomenology is explained and kinetically modeled to suggest a ping-pong mechanism of ROS generating activity where only dicarboxylate free reduced enzyme is oxidized by oxygen. The succinate:quinone reductase activity catalyzed by purified succinate:ubiquinone reductase also exhibits a ping-pong mechanism where only dicarboxylate free enzyme is oxidized by added quinone. Together these data suggest long distance interaction between the succinate (fumarate) binding and ubiquinone (ubiquinol) reactive sites.


Subject(s)
Electron Transport Complex II/metabolism , Reactive Oxygen Species/metabolism , Ubiquinone/metabolism , Animals , Cattle , Electron Transport Complex I/metabolism , Fumarates/metabolism , Kinetics , Mitochondria, Heart/metabolism , Multienzyme Complexes/metabolism , Oxidation-Reduction , Rats , Succinic Acid/metabolism , Superoxides/metabolism
15.
Adv Mater ; 28(36): 7978-7983, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27383739

ABSTRACT

Quasi-2D GaN layers inserted in an AlGaN matrix are proposed as a novel active region to develop a high-output-power UV light source. Such a structure is successfully achieved by precise control in molecular beam epitaxy and shows an amazing output power of ≈160 mW at 285 nm with a pulsed electron-beam excitation. This device is promising and competitive in non-line-of-sight communications or the sterilization field.

16.
FEBS Lett ; 588(18): 3441-4, 2014 Sep 17.
Article in English | MEDLINE | ID: mdl-25128457

ABSTRACT

Femtosecond absorption spectroscopy was applied to study for the first time excitation dynamics in isolated photosystem I trimers from Arthrospira platensis, which display extremely long-wavelength absorption peaks. Pump-probe spectra observed at 77K in the timescale of dozens of picoseconds upon 70-fs excitation revealed two maxima near 710 and 730 nm, which correspond to red chlorophyll forms. Bleaching at 680 nm developed in ∼ 200 fs, whereas the bleaching kinetics at 710 and 730 nm exhibited two components with time constants of 1 and 5.5 ps. Comparison of the kinetics of bleaching development at 710 nm and 730 nm with that of bleaching decay at 680 nm indicated that both long-wavelength forms of trimers are populated mainly via direct energy transfer from bulk chlorophyll.


Subject(s)
Bacteriochlorophylls/chemistry , Cyanobacteria , Photosystem I Protein Complex/chemistry , Energy Transfer , Kinetics , Spectrophotometry
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