ABSTRACT
The authors studied the concentration of CRP, sE-selectin, sP-selectin, sICAM-1, sICAM-3, sVCAM-1, sPECAM and endothelin-1 in blood serum of patients presenting with stenotic lesions of carotid arteries and undergoing various methods of carotid endarterectomy (CEAE): eversion CEAE (Group I) and CEAE using a xenopericardium patch (Group II). Within the time frame of the study, patients in both groups were found to have an elevated CRP level in the early postoperative period, having returned to the baseline values at 6 postoperative months, as well as an increase in the concentration of endothelin-1 at six months after surgery and a decrease of the sE-selectin concentration in the early postoperative period. The level of sP-selectin in Group II patients was noted to increase considerably six months after correction of stenosis. The content of sICAM-1 and sVCAM-1 did not differ in the early postoperative and baseline periods, and was noted to decrease 6 months after the operation. Group II patients demonstrated a decrease in the sPECAM concentration during postoperative day one, followed by returning to the initial values six months after CEAE. The above-mentioned biochemical markers may be used during the postoperative follow-up period for early detection and appropriate correction of endothelial dysfunction and hyperplasia of the intima of the zone of reconstruction.
Subject(s)
Biomarkers/blood , Carotid Stenosis , Endothelium, Vascular , Neointima , Postoperative Complications , Adult , Carotid Stenosis/blood , Carotid Stenosis/surgery , Early Diagnosis , Endarterectomy, Carotid/adverse effects , Endarterectomy, Carotid/methods , Endothelin-1/blood , Endothelium, Vascular/metabolism , Endothelium, Vascular/physiopathology , Female , Humans , Intercellular Adhesion Molecule-1/blood , Male , Middle Aged , Neointima/diagnosis , Neointima/etiology , Platelet Endothelial Cell Adhesion Molecule-1/blood , Postoperative Complications/blood , Postoperative Complications/diagnosis , Postoperative Period , Selectins/blood , Statistics as Topic , Vascular Cell Adhesion Molecule-1/bloodABSTRACT
INTRODUCTION: Our objective was to investigate the association between gene polymorphisms of folate cycle (MTHFR 677 C>T, MTHFR 1298 A>C, MTR 2756 A>G, and MTRR 66 A>G) and the risk of pulmonary embolism (PE) in a case-control study. MATERIALS AND METHODS: 177 PE patients (87 women and 90 men) were compared to a healthy control group (461 people, 123 women, 326 men). All of them are residents of Novosibirsk region. SNPs were genotyped by allele-specific PCR. RESULTS: The age distributions of our male and female patients were found to be significantly different. For men, the distribution has two maxima, whereas for women it has only one maximum, which is between the two. This fact stimulated us to perform a sex-specific analysis. No statistically significant difference has been found between distributions of the three genes in our PE patients and healthy controls. However, it was discovered that the TT genotype of MTHFR: 677 C>T polymorphism in men increases the risk of PE in comparison to controls. In fact, the difference increases in the age group over 45years. Also, AA genotype of MTRR 66 A>G polymorphism in women below 45years decreases the risk of PE. The sex-specific multiple linear regression analysis gave us estimates of the relative PE risk associated with MTHFR 677 C>T, F2: 20210G>A (Prothrombin), and F5: 1691G>A (Leiden) mutations.
Subject(s)
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase/genetics , Ferredoxin-NADP Reductase/genetics , Folic Acid/metabolism , Methylenetetrahydrofolate Reductase (NADPH2)/genetics , Polymorphism, Single Nucleotide , Pulmonary Embolism/genetics , Adult , Aged , Alleles , Case-Control Studies , Female , Genetic Predisposition to Disease , Genotype , Homocysteine/blood , Humans , Linear Models , Male , Middle Aged , Mutation , Polymerase Chain Reaction , Probability , SiberiaABSTRACT
The study was organized to evaluate sensitivity and specificity of CD64 index and relative amount of HLA-DR+ monocytes in diagnostic of sepsis in children of first year of life after surgery correction if congenital heart disease in conditions of artificial circulation. To detect CD64 index the kit Leuko64 (Beckman Coulter USA) was applied. The relative amount of HLA-DR+ monocytes was measured by flow cytofluorimeter Navios (Beckman Coulter, USA) using combination of monoclonal antibodies CD14-APC, HLA-DR-PacificBlue, CD45-KrOr. The results of study established that CD64 index in the group with confirmed or supposed sepsis consisted 2.29 (1.96:3.32) that statistically is reliably higher (p = 0.001) than in group without sepsis. The study established no statistically reliable differences in concentration of C-reactive protein in blood serum (p-0.123), absolute amount of leukocytes in peripheral blood (p = 0.128), relative amount of HLA-DR+ monocytes (p = 0.789). It is demonstrated that value of CD64 index higher than 2.00 increases the risk of development of sepsis up to 9.4 times and can be used as a diagnostic criterion of sepsis (AUC = 0.895) with sensitivity up to 80% and specificity up to 90%. The negative prognostic significance of CD64 index and content of procalcitonin in relation to development of sepsis in children of first year of life operated in conditions of artificial circulation amounted to 74% and 76% and 77% and 64% in case of positive prognostic significance correspondingly.
Subject(s)
Antigens, Differentiation/blood , Extracorporeal Circulation , Flow Cytometry , Heart Defects, Congenital , Biomarkers/blood , Female , Heart Defects, Congenital/blood , Heart Defects, Congenital/diagnosis , Heart Defects, Congenital/surgery , Humans , Infant , Infant, Newborn , Male , Sepsis/blood , Sepsis/etiologyABSTRACT
The article deals with an analysis of the literature data concerning immunological mechanisms of the formation of restenoses after damage of the arterial wall, considering the participants of the early and late phases of inflammatory response initiated by endothelial damage. Also given is characteristics of the process of formation on the neointima, followed by description of the role of intercellular adhesion molecules in initiation and maintaining of the processes of acute and chronic inflammation.
Subject(s)
Cell Adhesion Molecules/metabolism , Endothelium, Vascular , Graft Occlusion, Vascular/immunology , Acute-Phase Reaction/immunology , Endothelium, Vascular/immunology , Endothelium, Vascular/injuries , Endothelium, Vascular/physiopathology , Graft Occlusion, Vascular/physiopathology , Humans , Neointima/immunology , Vascular Patency/immunologyABSTRACT
The reagents kit is developed to identify and quantitatively detect DNA of methicillin sensitive and methicillin resistant Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus spp. in biological material using technique of polymerase chain reaction with hybridizational fluorescent detection and having higher analytical and diagnostic characteristics. The application of the given reagents kit makes it possible to optimize the epidemiologic monitoring of propagation of methicillin resistant strains of Staphylococcus spp. Significantly decreasing duration and laboriousness of study.
