1.
Angew Chem Int Ed Engl
; 55(10): 3290-4, 2016 Mar 01.
Article
in English
| MEDLINE
| ID: mdl-26844929
ABSTRACT
A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630â nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1â µm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60â nm.