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2.
PLoS Pathog ; 11(6): e1004984, 2015 Jun.
Article in English | MEDLINE | ID: mdl-26083414

ABSTRACT

All Staphylococcus aureus genomes contain a genomic island, which is termed νSaα and characterized by two clusters of tandem repeat sequences, i.e. the exotoxin (set) and 'lipoprotein-like' genes (lpl). Based on their structural similarities the νSaα islands have been classified as type I to IV. The genomes of highly pathogenic and particularly epidemic S. aureus strains (USA300, N315, Mu50, NCTC8325, Newman, COL, JH1 or JH9) belonging to the clonal complexes CC5 and CC8 bear a type I νSaα island. Since the contribution of the lpl gene cluster encoded in the νSaα island to virulence is unclear to date, we deleted the entire lpl gene cluster in S. aureus USA300. The results showed that the mutant was deficient in the stimulation of pro-inflammatory cytokines in human monocytes, macrophages and keratinocytes. Purified lipoprotein Lpl1 was further shown to elicit a TLR2-dependent response. Furthermore, heterologous expression of the USA300 lpl cluster in other S. aureus strains enhanced their immune stimulatory activity. Most importantly, the lpl cluster contributed to invasion of S. aureus into human keratinocytes and mouse skin and the non-invasive S. carnosus expressing the lpl gene cluster became invasive. Additionally, in a murine kidney abscess model the bacterial burden in the kidneys was higher in wild type than in mutant mice. In this infection model the lpl cluster, thus, contributes to virulence. The present report is one of the first studies addressing the role of the νSaα encoded lpl gene cluster in staphylococcal virulence. The finding that the lpl gene cluster contributes to internalization into non-professional antigen presenting cells such as keratinocytes highlights the lpl as a new cell surface component that triggers host cell invasion by S. aureus. Increased invasion in murine skin and an increased bacterial burden in a murine kidney abscess model suggest that the lpl gene cluster serves as an important virulence factor.


Subject(s)
Genomic Islands/genetics , Staphylococcal Infections/genetics , Staphylococcus aureus/genetics , Staphylococcus aureus/pathogenicity , Virulence/genetics , Animals , Cells, Cultured , DNA, Bacterial/genetics , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Female , Gene Knockout Techniques , Humans , Mice , Mice, Inbred BALB C , Microscopy, Electron, Transmission , Microscopy, Fluorescence , Virulence Factors/genetics
3.
J Invest Dermatol ; 135(3): 759-767, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25347115

ABSTRACT

In healthy human skin host defense molecules such as antimicrobial peptides (AMPs) contribute to skin immune homeostasis. In patients with the congenital disease ectodermal dysplasia (ED) skin integrity is disturbed and as a result patients have recurrent skin infections. The disease is characterized by developmental abnormalities of ectodermal derivatives and absent or reduced sweating. We hypothesized that ED patients have a reduced skin immune defense because of the reduced ability to sweat. Therefore, we performed a label-free quantitative proteome analysis of wash solution of human skin from ED patients or healthy individuals. A clear-cut difference between both cohorts could be observed in cellular processes related to immunity and host defense. In line with the extensive underrepresentation of proteins of the immune system, dermcidin, a sweat-derived AMP, was reduced in its abundance in the skin secretome of ED patients. In contrast, proteins involved in metabolic/catabolic and biosynthetic processes were enriched in the skin secretome of ED patients. In summary, our proteome profiling provides insights into the actual situation of healthy versus diseased skin. The systematic reduction in immune system and defense-related proteins may contribute to the high susceptibility of ED patients to skin infections and altered skin colonization.


Subject(s)
Ectodermal Dysplasia/immunology , Ectodermal Dysplasia/metabolism , Peptides/metabolism , Proteomics , Skin/metabolism , Adult , Animals , Case-Control Studies , Disease Models, Animal , Female , Gene Expression Profiling , Humans , Male , Mice , Mice, Inbred C57BL , Ointments , Peptides/administration & dosage , Peptides/therapeutic use , Staphylococcal Skin Infections/drug therapy , Staphylococcal Skin Infections/microbiology , Staphylococcus aureus , Sweat Glands/metabolism
4.
Exp Dermatol ; 22(2): 153-5, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23362876

ABSTRACT

Experimental mouse models of bacterial skin infections that have been described show that pathogenic microorganisms can readily invade the epidermis and dermis to produce localized infections. We used an epicutaneous mouse skin infection model to determine how the level of barrier disruption by tape-stripping correlates with persistence of Staphylococcus aureus skin colonization, concomitant induction of cutaneous inflammation and infection. Furthermore, we investigated how murine skin responds to S. aureus colonization in a physiologic setting by analysing proinflammatory cytokines and antimicrobial peptides in mouse skin. We show that previous cutaneous damage allows skin inflammation to develop and favours S. aureus persistence leading to cutaneous colonization, suggesting an interdependence of cutaneous bacteria and skin. Our study suggests that skin barrier defects favour S. aureus skin colonization, which is associated with profound cutaneous inflammation.


Subject(s)
Gene Expression Regulation, Bacterial , Inflammation/microbiology , Skin/microbiology , Staphylococcal Skin Infections/immunology , Animals , Biopsy , Colony-Forming Units Assay , Cytokines/immunology , Disease Models, Animal , Female , Keratinocytes/microbiology , Mice , Mice, Inbred C57BL , RNA, Messenger/metabolism , Skin/immunology , Skin/pathology
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