ABSTRACT
Antianxiety action of diterpene alkaloid songorine was studied using Vogel conflict test. Songorine in a dose of 0.25 mg/kg demonstrated high anxiolytic activity comparable to that of phenazepam and produced no sedative effect.
Subject(s)
Alkaloids/pharmacology , Anti-Anxiety Agents/pharmacology , Diterpenes/pharmacology , Stress, Psychological/drug therapy , Aconitum/chemistry , Alkaloids/isolation & purification , Animals , Anti-Anxiety Agents/isolation & purification , Behavior, Animal/drug effects , Benzodiazepines/pharmacology , Conflict, Psychological , Diterpenes/isolation & purification , Dose-Response Relationship, Drug , Male , Mice , Mice, Inbred CBA , Stress, Psychological/physiopathologyABSTRACT
We compared analgesic activities of individual alkaloids extracted from Baikal aconite (Aconitum baikalensis): napelline, hypaconitine, songorine, mesaconitine, 12-epinapelline N-oxide. The detected analgesic activity was comparable to that of sodium metamizole. The mechanisms of analgesia were different in diterpene alkaloids of different structure. The antinociceptive effect of atisine alkaloids (12-epinapelline N-oxide, songorine) was naloxonedependent and realized via opioid receptor modulation.
Subject(s)
Aconitum/chemistry , Analgesics/pharmacology , Pain/prevention & control , Seizures/prevention & control , Acetic Acid , Aconitine/analogs & derivatives , Aconitine/isolation & purification , Aconitine/pharmacology , Alkaloids/isolation & purification , Alkaloids/pharmacology , Analgesics/isolation & purification , Animals , Animals, Outbred Strains , Arthritis, Experimental/chemically induced , Arthritis, Experimental/physiopathology , Dipyrone/pharmacology , Freund's Adjuvant , Injections, Intraperitoneal , Mice , Pain/chemically induced , Pain/physiopathology , Plant Extracts/chemistry , Rats , Seizures/chemically induced , Seizures/physiopathology , Vocalization, Animal/drug effectsABSTRACT
We compared anti-inflammatory activity of individual diterpene alkaloids isolated from Aconitum baikalense (napelline, songorine, hypaconitine, mesaconitine, 12-epinapelline N-oxide) under conditions of acute inflammation of different genesis. The tested substances showed high antiexudative activity comparable with that of sodium diclofenac. Unlike nonsteroidal anti-inflammatory drugs, diterpene alkaloids exerted no ulcerogenic effect.
Subject(s)
Aconitum/chemistry , Anti-Inflammatory Agents/pharmacology , Diterpenes/pharmacology , Plant Extracts/pharmacology , Animals , Animals, Outbred Strains , Anti-Inflammatory Agents/therapeutic use , Diterpenes/therapeutic use , Drug Evaluation, Preclinical , Edema/chemically induced , Edema/drug therapy , Hindlimb/drug effects , Hindlimb/pathology , Mice, Inbred CBA , Plant Extracts/therapeutic useABSTRACT
A mixture of diterpene alkaloids of Aconitum baicalense exhibited pronounced regenerative hemostimulating properties on the model of cytostatic myelosuppression. The therapeutic effect is based on activation of hemopoietic progenitor cells against the background of increased feeder capacity of stromal compartment of the hemopoiesis-inducing microenvironment and decreased count of mesenchymal stem cells in the bone marrow.
Subject(s)
Aconitum/chemistry , Alkaloids/pharmacology , Diterpenes/pharmacology , Hematopoiesis/drug effects , Hematopoietic Stem Cells/drug effects , Regenerative Medicine/methods , Alkaloids/analysis , Animals , Bone Marrow Cells/drug effects , Cellular Microenvironment , Diterpenes/analysis , Fluorouracil , MiceABSTRACT
Pronounced regenerative effects of alkaloid napelline extracted from Aconitum baicalense on granulocytic hemopoiesis were revealed in the model of cytostatic myelosuppression. Activation of functions of hemopoietic progenitor cells along with increased feeder capacity of the stromal elements in the hemopoiesis inducing microenvironment with decreased levels mesenchymal progenitor cells in the bone marrow are the mechanisms underlying the hemostimulating action of this substance.
Subject(s)
Hematopoiesis/drug effects , Aconitine/pharmacology , Animals , Bone Marrow/drug effects , Bone Marrow Cells/drug effects , Bone Marrow Cells/physiology , Cells, Cultured , Cytostatic Agents/pharmacology , Fluorouracil/pharmacology , Mesenchymal Stem Cells/drug effects , Mesenchymal Stem Cells/metabolism , Mice, Inbred CBA , Myeloid Progenitor Cells/drug effects , Myeloid Progenitor Cells/physiology , Regenerative MedicineABSTRACT
Regeneratory activities of Baikal aconite alkaloids were studied on the excision skin wound model. Manifest wound healing effects of songorine, napelline, and hypaconitine were detected. The therapeutic efficiency was based on activation of residual mesenchymal progenitor elements. The development of this phenomenon was explained by the direct effects of alkaloids on precursors and by higher production of growth factors by the skin stromal cells. Songorine exhibited the most pronounced specific activity due to more significant stimulation of progenitor cell differentiation associated with maximum activation of the secretory function of the microenvironment cells.
Subject(s)
Aconitum/chemistry , Alkaloids/pharmacology , Diterpenes/pharmacology , Regeneration/drug effects , Skin/drug effects , Wound Healing/drug effects , Wounds and Injuries/drug therapy , Aconitine/analogs & derivatives , Aconitine/isolation & purification , Aconitine/pharmacology , Alkaloids/isolation & purification , Animals , Animals, Outbred Strains , Cell Differentiation/drug effects , Cellular Microenvironment/drug effects , Diterpenes/isolation & purification , Intercellular Signaling Peptides and Proteins/biosynthesis , Intercellular Signaling Peptides and Proteins/metabolism , Male , Mesenchymal Stem Cells/drug effects , Mice , Skin/injuries , Stromal Cells/drug effects , Wounds and Injuries/pathologyABSTRACT
Effects of the Yantar-Aantitox (succinic acid preparation) preparation on bioenergetic processes in mitochondria of rat liver during the experimental disorders of beta oxidation process evoked by 4-pentenoic acid have been studied. It is established that the course administration of Yantar-Antitox leads to normalization of disturbed bioenergetic processes in rat liver, which is due to stimulation of the rapid metabolic cluster of mitochondria.
