ABSTRACT
The study examined the effect of bone marrow multipotent mesenchymal stromal cells and their products secreted into the conditioned medium on uterine microcirculatory bed in Wistar rats during chronic inflammation. The parameters of blood and lymphatic microcirculation in the uterus changed in various directions in relation to the administration routes of biomedical cell product, which is important when the cell therapy is employed in the treatment of inflammatory-degenerative alterations in the lesser pelvis organs.
Subject(s)
Culture Media, Conditioned/pharmacology , Endometritis/pathology , Lymphatic Vessels , Mesenchymal Stem Cells/physiology , Microcirculation , Uterus , Animals , Bone Marrow Cells/physiology , Cells, Cultured , Chronic Disease , Culture Media, Conditioned/metabolism , Disease Models, Animal , Endometritis/therapy , Female , Lymphatic Vessels/drug effects , Lymphatic Vessels/physiology , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells/metabolism , Microcirculation/drug effects , Rats , Rats, Wistar , Uterus/blood supply , Uterus/drug effects , Uterus/pathologyABSTRACT
Numerical density of vessels in axillary lymph node structures in breast cancer patients was studied by light microscopy with antibodies to CD34. The correlation between the stage of breast cancer and vascularization of lymph nodes was analyzed. The development of breast cancer was followed by rapid and significant increase in the number of blood vessel in axillary lymph nodes in general and in all their specific areas, especially in the paracortex and medullary substance. Strong or very strong positive correlation between the stage of tumor process and parameters of vascularization was observed in various areas of the axillary lymph nodes. No significant differences in node vascularization were observed between women without breast cancer and women with stage I breast cancer; the differences were found only starting from stage II. Considerable vascularization of lymph nodes can be a symptom of malignant tumor growth in the area of lymph collection. The duration of the tumor process and the size of the tumor can be indirectly evaluated by the increase in vessel number in lymph nodes.
Subject(s)
Breast Neoplasms/blood supply , Lymph Nodes/blood supply , Neovascularization, Physiologic/physiology , Axilla , Breast Neoplasms/pathology , Female , Humans , Lymph Nodes/pathologyABSTRACT
Features of ultrasound picture of morphologically verified recurrence of ovarian cancer in 21 patients are presented, who received combined treatment including cytoreductive surgery in the form of hysterectomy with oophorectomy, resection of the greater omentum and 6 courses of chemotherapy CAP for ovarian cancer stage III (FIGO). In all patients cytoreductive surgery was optimal--without residual tumor. Recurrence of the disease was detected in 12-48 months in 80.9% of the cases. Three variants of recurrence was revealed by ultrasonography: isolated peritoneal dissemination, in 14.2% of the cases, which was mainly detected during the first 12 months; single entities in the projection of the small pelvis (61.9%) and mixed form (local lesions of small pelvis and peritoneal dissemination) in 23.8% of the cases.
Subject(s)
Hysterectomy , Neoplasm Recurrence, Local/diagnostic imaging , Omentum/surgery , Ovarian Neoplasms/diagnostic imaging , Ovarian Neoplasms/surgery , Ovariectomy , Peritoneal Neoplasms/diagnostic imaging , Adult , Aged , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Disease-Free Survival , Female , Humans , Middle Aged , Omentum/pathology , Ovarian Neoplasms/drug therapy , Peritoneal Neoplasms/secondary , Peritoneal Neoplasms/surgery , Survival Analysis , Time Factors , Treatment Outcome , UltrasonographyABSTRACT
Based on the study of 60 women who were treated at the gynecology department of the Novosibirsk Regional Oncology Dispensary from 2003 to 2013 with a diagnosis of primary multiple synchronous and metachronous neoplasia with endometrial lesion it was found that the most common risk factors for this disease could be considered: overweight and obesity; late age at onset of menarche; late age of menopause; concomitant cardiovascular diseases and disorders of the endocrine metabolism.
Subject(s)
Endometrial Neoplasms/diagnosis , Endometrial Neoplasms/epidemiology , Neoplasms, Multiple Primary/diagnosis , Neoplasms, Multiple Primary/epidemiology , Obesity/complications , Adult , Age Factors , Aged , Female , Humans , Menarche , Menopause , Middle Aged , Neoplasms, Second Primary/diagnosis , Neoplasms, Second Primary/epidemiology , Risk Factors , Russia/epidemiologyABSTRACT
For modulation of antitumor cytotoxic activity of mononuclear cells in vitro, autologous dendritic cells loaded with tumor lysate antigens were cultured with peripheral blood mononuclear cells from patients with epithelial ovarian cancer in the presence or absence of IL-12 and IL-18. The efficiency of modulation was evaluated by cytotoxic activity of mononuclear cells against autologous tumor cells, by the production of IFN-γ, IL-4, and by the count of perforin-containing lymphocytes. It was demonstrated that dendritic cells stimulated cytotoxic immune response in mononuclear cell culture. Maximum induction of cytotoxic activity of mononuclear cells was attained in case of dendritic cells combination with IL-12 and IL-18 (increased death of autologous tumor cells, accumulation of perforin-positive lymphocytes, enhanced production of IFN-γ).
