ABSTRACT
Conditions for efficient regeneration in mutant strains of the doxorubicin-producing organism Str. peucetius var. caesius were developed. The effect of the protoplast regeneration on changes in the proportion of the components of the anthracycline complex produced by these strains was shown. Variants with doxorubicin productivity 2 times higher than that of the parent strain were isolated.
Subject(s)
Antibiotics, Antineoplastic/biosynthesis , Protoplasts/physiology , Streptomyces/ultrastructure , Bacteriological Techniques , Daunorubicin/analogs & derivatives , Daunorubicin/biosynthesis , Doxorubicin/biosynthesis , Mutation , Selection, Genetic , Streptomyces/metabolismABSTRACT
Conditions for cultivation of basidiomycete Coprinus radiatus on agar media and its submersion fermentation were developed. An antibiotic was isolated from the fermentation broth filtrate by extraction with ethyl acetate followed by purification on a column with silica gel. The antibiotic had a pronounced in vivo effect on hemoblastosis La. Spectral analysis of the antibiotic (UV, IR, NMR and mass spectra) showed that it was identical to patulin (a toxin).
Subject(s)
Agaricales , Antineoplastic Agents/isolation & purification , Coprinus , Leukemia, Experimental/drug therapy , Agaricales/metabolism , Animals , Antineoplastic Agents/analysis , Antineoplastic Agents/therapeutic use , Chemical Phenomena , Chemistry, Physical , Coprinus/metabolism , Drug Evaluation, Preclinical , Fermentation , Mice , Structure-Activity Relationship , Time FactorsABSTRACT
In complementation analysis of low active streptidine dependent strains of Act. streptomycini, 170 and 145 with mutants having different blocks in biosynthesis of streptomycin it was found that these strains were the donors of some thermostable substances and could reduce the biosynthesis of streptomycin in the mutants having impairements in biosynthesis of streptidine and streptobiosamine, as well as in a number of strains with unknown blocks. It is supposed that the substances produced by mutants 170 and 145 were intermediate products in streptomycin biosynthesis.
Subject(s)
Guanidines/biosynthesis , Mutation/drug effects , Streptomyces griseus/metabolism , Streptomycin/antagonists & inhibitors , Cyclohexanols/biosynthesis , Genetic Complementation Test , Hexosamines , Molecular Conformation , Streptomyces griseus/genetics , Streptomycin/biosynthesisABSTRACT
Twelve mutants having a block in biosynthesis of the streptidine part of the streptomyciu molecule were selected under the effect of nitrozomethylbiuret. These 12 strains responded by an increase in the level of the antibiotic production to the addition of streptidine to the cultivation medium. The complementation analysis showed that every streptidine-dependent mutant interacted at least with 2 other mutants. On the basis of the data obtained it is possible to conclude that all 12 mutants had blocks in streptidine biosynthesis but at different stages of this complicated process.
Subject(s)
Bacterial Proteins/metabolism , Guanidines/chemical synthesis , Mutation , Streptomyces griseus/metabolism , Streptomycin/biosynthesis , Cyclohexanols/chemical synthesis , Genetic Complementation Test , Hexosamines , Methylnitrosourea/pharmacology , Mutation/drug effects , Streptomyces griseus/geneticsABSTRACT
Mutant 170 not capable of forming streptidine and streptomycin was obtained using chemical mutagenes. This mutant can produce streptomycin only with suplementation of exogenous streptidine. Experiment with labeled C14-streptidine showed its specific incorporation in streptidine moiety of streptomycin molecule.