ABSTRACT
The use of various types of stimulation of fish spermatozoa in order to increase the survival of defrosted cells is a promising direction in the field of low-temperature preservation. In this work, the use of acoustic-mechanical effects on the sperm of sturgeons (in particular, sterlet) was studied for the first time. The technical characteristics of the assembled experimental device for acoustic-mechanical effects on fish sperm are given. As a result of this work, the optimal parameters of exposure to increase the preservation of defrosted germ cells were selected.
Subject(s)
Cryopreservation , Semen , Acoustics , Animals , Fishes , Male , Sperm Motility/physiology , Spermatozoa/physiologyABSTRACT
The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from different regions are lactic acid bacteria (LAB; lactobacilli, lactococci, and Leuconostoc spp. in different ratios) and different types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia.
ABSTRACT
B-cell acute lymphoblastic leukemia (B-ALL) is a diverse group of malignant blood disorders both with regard to the biological properties of the tumor and to therapeutic approaches. Immunophenotyping, molecular genetic techniques, whole-genome sequencing characterize B-ALL as a very diverse group for sensitivity to chemotherapy and prognosis. We present three clinical cases of patients with B-ALL and expected good response to standard therapy, in whom standard protocol treatment failured: refractoriness, persistence of minimal residual disease (MRD), and progression (MRD increase). The remission in these patients was achieved after chemotherapy change to immunological targeted therapy. Nowadays a unified therapeutic approach to all primary patients of the B-ALL is considered generally outdated. Great efforts are carrying out to develop molecular genetic classifications. The molecular dissection of subtypes of B-ALL goes on, and new protocols for selective treatment with targeting are clearly outlined for each subtype of B-ALL.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Immunotherapy/methods , Philadelphia Chromosome , Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/diagnosis , Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Adult , Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Disease-Free Survival , Female , Humans , Male , Middle Aged , Neoplasm, Residual , Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/genetics , Remission InductionABSTRACT
Vascular dysregulation has long been recognized as an important pathophysiological factor underlying the development of glaucomatous neuropathy. Endothelin-1 (ET1) has been shown to be a key player due to its potent vasoconstrictive properties that result in retinal ischemia and oxidative stress leading to retinal ganglion cell (RGC) apoptosis and optic nerve (ON) damage. In this study we investigated the protective effects of magnesium acetyltaurate (MgAT) against retinal cell apoptosis and ON damage. MgAT was administered intravitreally prior to, along with or after administration of ET1. Seven days post-injection, animals were euthanized and retinae were subjected to morphometric analysis, TUNEL and caspase-3 staining. ON sections were stained with toluidine blue and were graded for neurodegenerative effects. Oxidative stress was also estimated in isolated retinae. Pre-treatment with MgAT significantly lowered ET1-induced retinal cell apoptosis as measured by retinal morphometry and TUNEL staining. This group of animals also showed significantly lesser caspase-3 activation and significantly reduced retinal oxidative stress compared to the animals that received intravitreal injection of only ET1. Additionally, the axonal degeneration in ON was markedly reduced in MgAT pretreated animals. The animals that received MgAT co- or post-treatment with ET1 also showed improvement in all parameters; however, the effects were not as significant as observed in MgAT pretreated animals. The current study showed that the intravitreal pre-treatment with MgAT reduces caspase-3 activation and prevents retinal cell apoptosis and axon loss in ON induced by ET1. This protective effect of ET1 was associated with reduced retinal oxidative stress.
Subject(s)
Apoptosis , Endothelin-1/administration & dosage , Neuroprotective Agents/administration & dosage , Optic Nerve Injuries/pathology , Retina/injuries , Retinal Ganglion Cells/pathology , Taurine/analogs & derivatives , Animals , Caspase 3/metabolism , Female , Intravitreal Injections , Male , Optic Nerve Injuries/chemically induced , Optic Nerve Injuries/metabolism , Oxidative Stress , Rats, Sprague-Dawley , Retina/drug effects , Retinal Ganglion Cells/drug effects , Retinal Ganglion Cells/metabolism , Taurine/administration & dosageABSTRACT
We develop a new approximation for the amplitude reflection coefficients of a slightly inhomogeneous thin film. This approximation incorporates exactly the interference effects at the substrate and the ambient interfaces. Interference effects inside the inhomogeneous film are incorporated in the Born approximation. We also develop a new approach to the reconstruction of the refractive-index profile from ellipsometric spectra. It is based on a physically sound parameterization of the refractive-index profile. The new approach is tested on the model reconstruction problem.