ABSTRACT
1.6% of clinically healthy women active in certain professions have been found to be carriers of pathogenic species of Listeria. The study of the sensitivity of 73 strains of Listeria spp. to the preparations of Chlorophyllipt has revealed that in usual therapeutic concentrations Chlorophyllipt produces a pronounced antibacterial effect on these organisms. The use of Chlorophyllipt for the sanitation of carriers of pathogenic Listeria is proposed.
Subject(s)
Carrier State/drug therapy , Listeriosis/drug therapy , Occupational Diseases/drug therapy , Adjuvants, Immunologic/therapeutic use , Anti-Bacterial Agents/therapeutic use , Belgium , Carrier State/microbiology , Chlorophyll/therapeutic use , Drug Combinations , Environmental Microbiology , Female , Humans , Listeria/isolation & purification , Listeriosis/microbiology , Occupational Diseases/microbiology , Plant Extracts/therapeutic use , UkraineABSTRACT
Data on the isolation of Listeria spp. cultures on the territory of Ukraine, the frequency of Listeria contamination of alimentary raw materials and foodstuffs, the level of listeriosis morbidity, the clinical forms of the manifestation of this disease, as well as the occurrence of asymptomatic carriership among clinically healthy persons of epidemiologically significant professions, are given. The results of the study of the biological properties of 73 strains of Listeria spp., their sensitivity to antibiotics and Chlorophyllipt are presented.
Subject(s)
Listeriosis/epidemiology , Animals , Antigens, Bacterial/classification , Bacteriophage Typing , Environmental Microbiology , Humans , Listeria/classification , Listeria/drug effects , Listeria/isolation & purification , Listeria/pathogenicity , Listeriosis/microbiology , Listeriosis/transmission , Microbial Sensitivity Tests , Serotyping , Ukraine/epidemiologyABSTRACT
A system of primers for detection of Listeria by polymerase chain reaction (PCR) has been developed. Specificity and sensitivity of the method was evaluated by analyses of reference clinical and abiotic samples. Clinical trials of PCR were carried by detecting latent (asymptomatic) carriers of Listeria among healthy women of epidemiologically significant professions. PCR is characterized by numerous advantages in comparison with the traditional bacteriological analysis.
Subject(s)
Listeria/isolation & purification , Listeriosis/diagnosis , Listeriosis/microbiology , Polymerase Chain Reaction/methods , DNA, Bacterial/analysis , Female , Humans , Listeria/genetics , Sensitivity and SpecificityABSTRACT
The weight (volume) of inoculate applied with Semenikhina's replicator stamp with 1, 2, and 3-mm needles is determined. The method is sufficiently precise for microbiological screenings. The volume of inoculate depends on the diameter and geometrical shape of the needle and characteristics of inoculated substance and surface onto which it is applied.
Subject(s)
Microbiological Techniques/instrumentation , Culture Media , Equipment Design , Filtration/instrumentation , Microbiological Techniques/statistics & numerical data , Needles , Reproducibility of ResultsABSTRACT
Biological characteristics of 21 strains of bacteria of Listeria genus have been studied in Ukraine and Belgium. Morphological, cultural biochemical properties of cultures have been described. It has been proved that 85.7% produced bacteriocins. Antigenic titration of Listeria has been conducted and sensitivity to Listeria bacteriophage 2 LA and 4 LA has been estimated.
Subject(s)
Listeria/physiology , Animals , Animals, Domestic/microbiology , Animals, Wild/microbiology , Anti-Bacterial Agents/pharmacology , Antigens, Bacterial/analysis , Bacteriocins/biosynthesis , Bacteriophage Typing , Belgium , Humans , Listeria/classification , Listeria/drug effects , Listeria/immunology , Listeria/isolation & purification , Listeriosis/microbiology , Microbial Sensitivity Tests , UkraineSubject(s)
Brachial Plexus/injuries , Forearm/blood supply , Adolescent , Adult , Arteries/injuries , Arteries/surgery , Brachial Plexus/surgery , Child , Female , Follow-Up Studies , Humans , Male , Microsurgery/methods , Middle AgedABSTRACT
Remote results of the treatment of 68 patients with an associated trauma of the forearm were studied. Complete recovery of the hand functioning was noted in 63,2% of the patients. In 22% of the patients with severe associated traumas the extremity function was considerably worse despite the reestablished blood circulation. Repeated operations on the nerve were indicated to 5,8% of the patients.
