ABSTRACT
Agromyces albus sp. nov. is proposed for an aerobic, oxidase- and catalase-positive actinomycete that was isolated from the above-ground part of a plant (Androsace sp., in the family Primulaceae). The strain is characterized by white colonies, fragmenting hyphae that penetrate into agar media and chemotaxonomic properties that are typical of the genus AGROMYCES: Analysis of 16S rDNA sequences confirmed that the strain belongs to the genus Agromyces and revealed its close phylogenetic relationship with Agromyces ramosus. DNA-DNA pairing studies showed that the strain belongs to a separate genomic species; this is consistent with its distinction from other Agromyces species at the phenotypic level. The G+C content of the DNA was 69.0 mol%. The type strain is VKM Ac-1800(T) (=UCM Ac-623(T)).
Subject(s)
Actinomycetales/isolation & purification , Primulaceae/microbiology , Actinomycetales/classification , Actinomycetales/genetics , Actinomycetales/metabolism , Base Composition , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Molecular Sequence Data , Phenotype , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/geneticsABSTRACT
Dichloromethane (DCM) is a toxic pollutant showing prolonged persistence in water. DCM biodegradation is usually determined from increases in Cl ions, gas chromatography, or by using radioisotopes. Herein, we present an original and easy spectrophotometric method to estimate DCM concentrations in cultures and environmental samples during DCM biodegradation experiments.
Subject(s)
Methylene Chloride/metabolism , Methylobacterium/metabolism , Water Pollutants, Chemical/metabolism , Biodegradation, Environmental , Environmental Monitoring/methods , Spectrophotometry, Ultraviolet , Water MicrobiologyABSTRACT
Okibacterium fritillariae gen. nov., sp. nov. (type strain VKM Ac-2059T = IFO 16404T) is proposed for aerobic, oxidase- and catalase-positive, coryneform bacteria isolated from seeds of Fritillaria ruthenica Wikstr. and Clematis recta L. Strains of the new genus form a distinct branch within the phylogenetic cluster composed of members of the family Microbacteriaceae and are characterized by B-type peptidoglycan containing amino acids glycine, glutamate, homoserine, alanine and lysine, the glycolyl type of muramic acid, the major menaquinones MK-10 and MK-11, the principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, and a DNA G+C content of approximately 67 mol %.
Subject(s)
Actinomycetales/classification , Clematis/microbiology , Fritillaria/microbiology , Seeds/microbiology , Actinomycetales/chemistry , Actinomycetales/genetics , Actinomycetales/physiology , Bacterial Typing Techniques , DNA, Ribosomal/analysis , Molecular Sequence Data , Peptidoglycan/analysis , Peptidoglycan/chemistry , Phospholipids , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analysisABSTRACT
Two novel species, Rathayibacter caricis sp. nov. (type strain VKM Ac-1799T = UCM Ac-618T) and Rathayibacter festucae sp. nov. (type strain VKM Ac-1390T UCM Ac-619T), are proposed for two coryneform actinomycetes found in the phyllosphere of Carex sp. and in the leaf gall induced by the plant-parasitic nematode Anguina graminis on Festuca rubra L., respectively. The strains of the novel species are typical of the genus Rathayibacter in their chemotaxonomic characteristics and fall into the Rathayibacter 16S rDNA phylogenetic cluster. They belong to two separate genomic species and differ markedly from current validly described species of Rathayibacter at the phenotypic level. The most striking feature differentiating Rathayibacter caricis sp. nov. from other species of the genus is the presence of fucose in its cell wall and Rathayibacter festucae sp. nov. can be easily recognized among other yellow-pigmented rathayibacters because of its rose-orange-coloured colonies.
