Bioavailability and allergoprotective capacity of milk-associated conjugated linoleic acid in a murine model of allergic airway inflammation.

BACKGROUND: Cross-sectional epidemiological studies have demonstrated that farm milk from traditional farm settings possesses allergoprotective properties. Up to now, it has not been clarified which milk ingredient is responsible for protection against allergic diseases. As farm milk is rich in conjugated linoleic acids (CLA), it is hypothesized that this n-3 polyunsaturated fatty acid family contributes to the allergoprotective capacity of farm milk. We aim to prove this hypothesis in a murine model of allergic airway inflammation. METHODS: To prove the bioavailability and allergoprotective capacity of milk-associated CLA in a standardized protocol, milk batches that differed significantly in terms of their CLA content were spray dried and incorporated into a basic diet by substituting the regular sunflower fat fraction. Initially, the milk CLA uptake from the diet was monitored via measurement of the CLA content in plasma and erythrocyte membranes obtained from supplemented mice. To determine whether a milk CLA-enriched diet possesses allergoprotective properties, female Balb/c mice were fed the milk CLA-enriched diet ahead of sensitization and a challenge with ovalbumin (OVA) and the parameters of airway inflammation and eisosanoid pattern were measured. RESULTS: In animals, supplementation with a diet rich in milk CLA resulted in elevated CLA levels in plasma and erythrocyte membranes, indicating bioavailability of milk fatty acids. Though membrane-associated phospholipid patterns were affected by supplementation with milk CLA, this application neither reduced the hallmarks of allergic airway inflammation in sensitized and OVA-challenged mice nor modified the eiconsanoid pattern in the bronchoalveolar lavage fluid of these animals. CONCLUSION: Milk-associated CLA was not capable of preventing murine allergic airway inflammation in an animal model of OVA-induced allergic airway inflammation.

Evaluation of alternative polymer bracket materials.

INTRODUCTION: Polymer brackets still have some disadvantages because of decreased wear resistance and hardness. The aim of this study was to investigate the mechanical properties of alternative bracket polymers; urethane-dimethacrylate, high-density polyethylene, and an experimental bracket polymer (EBP) consisting of polyethylene and a copolymer were tested. Polycarbonate and polyoxymethylene brackets served as controls. METHODS: The mechanical properties of urethane-dimethacrylate, high-density polyethylene, EBP, polycarbonate, and polyoxymethylene bracket materials were evaluated after thermocycling (6000 x 5 degrees C-55 degrees C) in a mastication device before testing. Three medium-wear, fracture toughness, and Vickers hardness tests were performed. RESULTS: High-density polyethylene had the highest values of wear and the lowest values of fracture toughness and Vickers hardness. The urethane-dimethacrylate bracket material and the EBP had better mechanical properties than polycarbonate. The polyoxymethylene bracket material had the highest values of fracture toughness and Vickers hardness, and the lowest values of wear compared with the other investigated polymers. CONCLUSIONS: High-density polyethylene seems to be unsuitable as bracket material because it demonstrated excessive wear and insufficient fracture toughness. Polyoxymethylene had the best performance during mechanical testing.