ABSTRACT
The method of electret-thermal analysis developed in dielectric physics has been applied to monitor bioelectric phenomena that accompany the immobilization of microorganisms on electret substrates. A spectrum of thermo stimulated currents for biofilms consisting of immobilized Saccharomyces cells has been obtained. The spectrum represents a halo in the region of 30-90 degrees C. The immobilization of Saccharomyces cells on electret polyethylene films results in their depolarization that is recorded on the spectra of thermostimulated currents as a reduction of the current peak corresponding to the polyethylene melting point. A hypothesis has been put forward that explains the phenomenon by the absorption of the electric energy of polarized substrates by the cells for the occurrence of metabolic reactions.
Subject(s)
Biofilms , Polyethylene , Saccharomyces cerevisiae/metabolism , Cells, Immobilized/cytology , Cells, Immobilized/metabolism , Electrochemistry , Saccharomyces cerevisiae/cytologyABSTRACT
The thermally stimulated discharge (TSD) method, intended for the analysis of charged dielectrics, was used for human blood research. Above-room-temperature TSD spectra of blood consist of three peaks. There are indications that the low-temperature peak (40-50 degrees C) corresponds to the thermally stimulated destruction of hydrate shells surrounding blood components while the mid-temperature peak (70-90 degrees C) is related to thermal denaturation of blood proteins. The intensive high-temperature peak (105-120 degrees C) is observed when a phase transition of blood, accompanied by the formation of a firm dry film of blood, occurs. The position of the high-temperature peak depends on the blood group. Data is discussed which suggests that the spontaneous "quasi-electret effect" of blood relates to the character of the biochemical processes taking place in human organisms. The TSD method might be used as simple and informative means of diagnostics in cooperation with medical and physical investigations.
Subject(s)
Blood Chemical Analysis/instrumentation , Blood Chemical Analysis/methods , Blood Group Antigens/classification , Electric Capacitance , Adult , Aluminum , Blood Group Antigens/chemistry , Female , Hot Temperature , Humans , Ion-Selective Electrodes , Male , Polytetrafluoroethylene , Sensitivity and SpecificityABSTRACT
Transmyoplasty was used in the operative treatment of 118 patients with chronic osteomyelitis of the lower extremity bones in the muscle-free zones and with scarry regeneration of the muscles. Good outcomes were obtained in 92% of the patients.
