ABSTRACT
A study was conducted on 60 psychiatric patients to evaluate the reliability of a drug-exposure screening test based on gas chromatography/mass spectrometry analysis of hair samples for amitriptyline, and the possibility of using the hair concentrations of amitriptyline to monitor patients' therapeutic compliance. The qualitative test was found to be very reliable (sensitivity 93.3%; specificity 100%; positive and negative predictive values 100 and 93.8%) in assessing the consumer status of patients over a period of 2 months before analysis. Hair levels of amitriptyline ranged from 0 to 17.21 ng mg-1. A significant relationship (r = 0.563; P < 0.002) was found between the hair concentrations and the cumulative intake of amitriptyline over the studied period, but was not judged close enough to estimate one individual's therapeutic compliance. The results are discussed in the light of existing literature.
Subject(s)
Amitriptyline/analysis , Hair/chemistry , Mental Disorders/metabolism , Adolescent , Adult , Aged , Amitriptyline/administration & dosage , Female , Humans , Male , Middle AgedABSTRACT
A high performance liquid chromatographic method with diode-array detection (HPLC/DAD) for simultaneous screening of 25 antidepressants is presented. After single-step liquid-liquid extraction at pH9.5 using chloroform/2-propanol/n-heptane (60/14/26, v/v), the substances are eluted on a Novapak C18 4-microns column (300 x 3.9 mm, id), with methanol/tetrahydrofuran/pH 2.6 phosphate buffer (65/5/30, v/v) as the mobile phase (flow rate 0.8 ml/min). Full UV spectra from 200 to 400 nm are recorded on-line during the entire analysis and may be automatically compared to spectra stored in a library. The analysis is performed in 14 min. The method is simple, rapid, and highly specific.
Subject(s)
Antidepressive Agents/blood , Antidepressive Agents/urine , Chromatography, High Pressure Liquid/methods , Antidepressive Agents/classification , Antidepressive Agents/poisoning , Humans , Poisoning/blood , Poisoning/urine , Spectrophotometry, UltravioletSubject(s)
Lung Neoplasms/secondary , Pseudomyxoma Peritonei/complications , Adult , Female , HumansABSTRACT
The influence of different oxygen content in the inhaled air was examined in 21 healthy male volunteers using noninvasive methods. Tidal volume, breathing frequency, minute ventilation, heart period duration, stroke volume factor, cardiac output factor, arterial blood pressure, and total peripheral resistance factor were measured. It is shown that the reactions of the cardiovascular parameters to a decrease of oxygen content in inhaled air were more sensitive than those of ventilatory parameters.
