[Dynamics of immunologic indicators in children with severe forms of suppurative-septic diseases after the exchange-substitute plasmapheresis]. / Dinamika immunologicheskikh pokazatelei u detei s tiazhelymi formami gnoino-septicheskikh zabolevanii pod vliianiem obmeno- zamestitel'nogo plazmafereza.

Time course of the immunity system parameters was followed in children suffering from grave endotoxicosis forms treated by discrete plasmapheresis (DPA) as the emergency therapeutic measure. T, T-active, B lymphocytes, O cells, immunoglobulins A, G, M were analyzed, as were the counts of phagocytizing leukocytes and circulating immune complexes. The infectious process was found to lead to a temporary relative secondary immunodeficiency. DPA with perfusion of hyperimmune plasma resulted in rapid correction of all component of the immunity system, paralleled by normalization of the general clinical status of the patients, and prevented chronic transformation of the process.

[The use of immunological method for the determination of acid alpha-glucosidase activity in human muscles]. / Ispol'zovanie immunologicheskogo metoda dlia opredeleniia aktivnosti kisloi alpha-gliukozidazy v myshtsakh cheloveka.

Homogeneous preparation of acid alpha-glucosidase, obtained from human placenta, was used for immunization of rabbits and production of specific antiserum. Immunological procedure was developed for estimation of acid alpha-glucosidase activity from human muscles in presence of the enzyme neutral form. Activity of acid alpha-glucosidase was measured in 20 patients with various types of nervous system impairments. Total activity of alpha-glucosidase in muscles of the patients was 11.4 +/- 0.7 nM/min/g of the tissue and activity of the acid form constituted about 84.8 +/- 1.7% of the total activity.

[Nature of the change in insulin receptors on the lymphocytes in a tumor-bearing organism]. / Kharakter izmeneniia insulinovykh retseptorov na limfotsitakh v opukholevom organizme.

Rat spleen lymphocytes were shown to bind insulin in vitro. This reaction which comes to saturation depends on both pH and temperature. In competition experiments two types of binding sites differing in their ability to bind insulin were revealed. In Zajdela hepatoma-bearing rats both Kass values and lymphocytes ability to bind insulin were increased, whereas in hepatoma G-27 bearing rats the number of receptors on lymphocytes was drastically decreased. On the second day of Zajdela hepatoma growth the number of receptors on lymphocyte was decreased 1.5-fold, then it was increased reaching the maximum up to fifth day with 5-fold augmentation. The changes in insulin-binding ability of lymphocytes were found to correlate with insulin-binding of the same rat liver plasma membranes. This finding indicates that the character of changes in insulin-binding ability is similar in various tissues of tumor-bearing rats.