ABSTRACT
Interpenetrating polymeric network nanogels (IPN-NGs) composed of natural gelatin biological protein macromolecules and poly(acrylamidoglycolic acid) were produced by simple free radical emulsion polymerization. The developed IPN-NGs were characterized by Fourier-transform infra-red spectroscopy to confirm the formation of NGs. The hydrophobic curcumin drug was loaded successfully into these NGs using an in-situ method. The curcumin-encapsulated NGs were well dispersed in aqueous solutions and showed good bioavailability. Curcumin was dispersed molecularly in the IPN-NGs, which was confirmed by differential scanning calorimetry and X-ray diffraction. The NGs exhibited pH sensitive properties according to dynamic light scattering and the zeta size potentials. Transmission electron microscopy revealed the NGs to be spherical, approximately 100nm in size. The encapsulation efficiency of these IPN-NGs drug formulations ranged from 42 to 48%. In addition, the release of curcumin from the NGs was examined in phosphate buffer medium. The cytotoxicity of the IPN-NGs was studied using in vitro cultures of fibroblasts and a colorectal cancer cell line. The results suggest that the newly developed pH sensitive gelatin-poly(acrylamidoglycolic acid)-curcumin NGs can be applied for colorectal cancer drug delivery applications.
Subject(s)
Antineoplastic Agents/chemistry , Curcumin/chemistry , Drug Delivery Systems , Gelatin/chemistry , Nanostructures/chemistry , Polymers/chemistry , Acrylamides/chemistry , Antineoplastic Agents/administration & dosage , Cell Survival/drug effects , Curcumin/administration & dosage , Fibroblasts/drug effects , Gels , Glycolates/chemistry , HCT116 Cells , Humans , Hydrogen-Ion Concentration , Nanostructures/administration & dosage , PolymerizationABSTRACT
A series of novel nanogels (NGs) with both pH and thermoresponsive properties were synthesised by free radical emulsion polymerisation of N-vinyl caprolactam (VCL) and acrylamidoglycolic acid (AGA). 5-Flurouracil, an anti cancer drug, was successfully loaded into these nanogels via equilibrium swelling method. The encapsulation efficiency of 5-FU was found up to 61%. Here we present the novel potential drug delivery system showing both pH and temperature release of 5-FU. Fourier transforms infrared spectroscopy (FTIR), and differential scanning calorimetric (DSC) examined the structure and morphology of the NGs. Transmission electron microscopy (TEM) indicates the diameter of the NGs to be about 50 nm. The size distribution of NGs was investigated using dynamic light scattering (DLS), the average diameter and polydispersity is 57 nm and 0.194. Interestingly, the in vitro release studies of 5-FU demonstrated the dual nature (pH and temperature) of NGs. The cumulative release data were analysed using an emperical equation to compute the diffusion exponent (n); whose values suggest Fickian diffusion.
Subject(s)
Antineoplastic Agents/chemistry , Caprolactam/analogs & derivatives , Polyethylene Glycols/chemistry , Polyethyleneimine/chemistry , Polymers/chemistry , Caprolactam/chemistry , Fluorouracil/chemistry , Hydrogen-Ion Concentration , Nanogels , TemperatureABSTRACT
New chitosan nanocomposites doped with silver nanoparticles were synthesized by a simple method. The chitosan particles were prepared by desolvation followed by crosslinking with poly(ethylene glycol-di-aldehyde), this was prepared with poly(ethylene glycol) in the presence of a silver nitrate solution. The developed nanocomposites were characterized using UV-visible, FTIR, XRD, TGA, SEM and TEM to understand their physico-chemical properties. These nanocomposites were shown to have anti bacterial activity towards Escherichia coli.
