ABSTRACT
OBJECTIVE: To evaluate the anticancer activity of the methanolic extract of Cyathula prostrata in Ehrlich ascites carcinoma (EAC)-bearing mice with methotrexate as positive control in the advanced stage of tumorigenesis. METHODS: EAC was induced in swiss albino mice by injecting 10(6) cell/mL of tumor cell suspension intraperitoneal. The methanolic extract of Cyathula prostrata effect on the tumor cell viability, DNA fragmentation and MTT assay were carried out. RESULTS: Methanolic extract attenuated percentage increased in the cell survival time when compared to control group. However, the effect was less than that of methotrexat. Methotrexat and the extracts reversed the tumor-induced alterations in DNA fragmentation and MTT assay. CONCLUSIONS: The present study suggests that the methanol extract of Cyathula prostrata has significant anticancer activity and that is comparable to that of methotrexate.
Subject(s)
Amaranthaceae/chemistry , Antineoplastic Agents, Phytogenic/administration & dosage , Carcinoma, Ehrlich Tumor/drug therapy , Plant Extracts/administration & dosage , Animals , Carcinoma, Ehrlich Tumor/genetics , Carcinoma, Ehrlich Tumor/physiopathology , Cell Line, Tumor , Cell Survival/drug effects , DNA Fragmentation/drug effects , Female , Humans , MiceABSTRACT
This study was aimed at evaluating the preventive role of the ethanolic extract of Lagenaria siceraria (Mol) fruit on membrane-bound enzymes, such as sodium potassium-dependent adenosine triphosphatase (Na(+)/K(+) ATPase), calcium-dependent adenosine triphosphatase (Ca(2+) ATPase) and magnesium-dependent adenosine triphosphatase (Mg(2+) ATPase) on isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Male albino Wistar rats were pretreated with the ethanolic extract of L. siceraria (Mol) fruit (125, 250 and 500 mg kg(-1) body weight) for a period of 30 days. After the treatment period, ISO (85mg kg(-1) body weight) was subcutaneously injected into rats at 24-h intervals for 2 days. ISO-induced rats showed a significant (p < 0.05) decrease in the activity of Na(+)/K(+) ATPase and an increase in the activities of Ca(2+) and Mg(2+) ATPases in the heart tissues. Pre-treatment with the ethanolic extract of L. siceraria (Mol) fruit for a period of 30 days exhibited a significant (p < 0.05) effect in ISO-induced rats. Thus, our study shows that the ethanolic extract of L. siceraria (Mol) fruit has membrane-stabilising role in ISO-induced MI in rats.
Subject(s)
Adrenergic beta-Antagonists/toxicity , Cucurbitaceae/chemistry , Heart/drug effects , Isoproterenol/toxicity , Animals , Cell Membrane/drug effects , Cell Membrane/enzymology , RatsABSTRACT
Diabetes mellitus is the most severe metabolic pandemic of the 21(st) century, affecting essential biochemical activities in almost every cell in the body. Indian literatures have already mentioned herbal remediation for a number of human ailments. The present study was undertaken to evaluate the potential of Coccinia grandis extract on serum and liver marker enzymes (ALP, AST, ALT and LDH) and lipid profile (total cholesterol, phospholipids, triglycerides and free fatty acids in serum and liver) in streptozotocin induced diabetic animals. The experimental animals were treated with methanolic extract of Coccinia grandis and the levels of marker enzymes and lipid profile were estimated. The ALP, AST, ALT and LDH levels were increased in diabetic rats and restored to near normal levels after administration of plant extract. The lipid profile increased in diabetic group and after the treatment with the plant extract the levels were reverted to near normal. Thus the methanolic extract of Coccinia grandis has a potent ability to restore the marker enzymes and the lipid profile was reverted to near normal levels.
ABSTRACT
The effect of Achyranthes aspera on lipid peroxidation were studied in rats fed with Sesame Oil. Increase in the levels of LPO in sesame oil treated groups returned towards normalcy in the plant extract treated groups revealing the antioxidant potential of the plant. Phytochemical studies revealed the presence of secondary metabolites. According to the results obtained Achyranthes aspera inhibited Ferrous Ascorbate stimulated LPO.
ABSTRACT
The present study was designed to evaluate the antihyperlipidemic effect of aqueous extract of Achyranthes aspera a in experimental rats fed with diet containing sesame oil. Hyperlipidemia and the effect of Achyranthes aspera in experimental rats were studied by assessing parameters such as cholesterol, phospholipids, freefattyacids and triglycerides in serum, liver, and heart and kidney tissues. The levels of HDL, LDL, VLDL and atherogenic index were assessed. Hyperlipidemia in experimental rats were evidenced by a significant enhancement in the levels of cholesterol phospholipids, freefattyacids and triglycerides in serum, liver heart and kidney tissues by atherogenic diet feeding. A significant fall in HDL in both Anjali and Idhayam oil treated groups were observed in serum. These values retrieved towards normalcy in Achyranthes aspera treated groups. This study unveiled the antihyperlipidemic activity of Achyranthes aspera.
ABSTRACT
The present study was carried out to evaluate the antidiabetic effect of T. arjuna stembark extract and to study the activities of hexokinase, aldolase and phosphoglucoisomerase, and gluconeogenic enzymes such as glucose-6-phosphatase and fructose-1,6-diphosphatase in liver and kidney of normal and alloxan induced diabetic rats. Oral administration of ethanolic extract of bark (250 and 500mg/kg body weight) for 30 days, resulted in significant decrease of blood glucose from 302.67±22.35 to 82.50±04.72 and in a decrease in the activities of glucose-6-phosphatase, fructose-1,6-disphosphatase, aldolase and an increase in the activity of phosphoglucoisomerase and hexokinase in tissues. However, in the case of 250 mg/kg body weight of extract, less activity was observed. The study clearly shows that the bark extract ofT. arjuna possesses potent antidiabetic activity.
ABSTRACT
Insight of evidence that some complications of diabetes mellitus due to hyperglycemia, we investigated the effect of T. arjuna bark extract on serum, liver and kidney marker enzymes in alloxan - induced diabetic rats. T. arjuna was administered orally at a doses of 250 and 500 mg/kg body weight for 30 days, after which serum liver and kidney tissues were assayed for the degree of pathological changes by means of markers such as alkaline phosphatase (ALP), acid phosphatase (ACP), alanine amino transferase (ALT), aspartate amino transferase (AST) and lactate dehydrogenase (LDH) resulted in a significant reduction in serum and tissue of liver and kidney marker enzymes when compared with control rats T. arjuna at a dose of 500 mg/kg body weight exhibited higher efficacy.
ABSTRACT
C.halicacabum is wide spread in tropical and sub-tropical Asia and Africa. Our laboratory results showed crude ethanolic extract of this plant exerted anti-inflammatory activity in chronic inflammatory models. In this present study, we tried to investigate the presence of anti-inflammatory compound in this extract.
ABSTRACT
Five varieties of minor millets were studied for their amylose, soluble amylose, amylopectin, soluble amylopectin, reducing sugar, total sugar and starch contents. Pure starch was isolated from each variety and the enzymic degradation of starch by porcine pancreatic alpha-amylase were examined with and without gelatinisation. Gelatinised sample of Echinochloa frumentacea (var. K2) showed minimal hydrolysis and gelatinised sample of Panicum miliaceum (var. CO3) showed maximum hydrolysis of starch by porcine pancreatic alpha-amylase. Gelatinised starch was highly susceptible to enzymic digestion when compared to ungelatinised starch. The extent of starch degradation varied from 71 to 85 percent in gelatinised samples and starch degradation in ungelatinised sample varied from 10 to 18 percent.
