ABSTRACT
Properties of a transplantable rat histiocytoma which behaves like a macrophage-like cell, have been described. The AK-5 grows as ascites as well as solid subcutaneous tumor. The ascites from one animal can give between 10(8) and 10(9) cells whereas the subcutaneous tumor grows between 20 and 40 cm3 size. These cells possess various degradative enzymes, macrophage markers and glucocorticoid receptors. Beside histopathology the surface topography and ultrastructure of these cells are described.
Subject(s)
Histiocytoma, Benign Fibrous/ultrastructure , Macrophages/ultrastructure , Tumor Cells, Cultured , Animals , Cell Division , Female , Male , Rats , Rats, Inbred Strains , Tumor Cells, Cultured/ultrastructureSubject(s)
Contraceptives, Oral, Combined/pharmacology , Contraceptives, Oral/pharmacology , Ethynodiol Diacetate/pharmacology , Liver Regeneration/drug effects , Animals , DNA/metabolism , Female , Hepatectomy , Lipid Metabolism , Liver/cytology , Liver/drug effects , Liver/metabolism , Proteins/metabolism , RNA/metabolism , Rats , Rats, Inbred StrainsSubject(s)
Ascites/etiology , Disease Outbreaks , Food Contamination/analysis , Plant Poisoning/complications , Pyrrolizidine Alkaloids/analysis , Adolescent , Adult , Child , Child, Preschool , Edible Grain , Female , Humans , India , MaleSubject(s)
Dietary Fats/adverse effects , Heart/drug effects , Macaca/metabolism , Oils/pharmacology , Alanine Transaminase/blood , Animal Nutritional Physiological Phenomena , Animals , Arachis , Aspartate Aminotransferases/blood , Cholesterol/blood , Dietary Fats/administration & dosage , Endomyocardial Fibrosis/chemically induced , Fatty Acids/analysis , Fatty Acids, Unsaturated/adverse effects , Haplorhini , Heart/anatomy & histology , Liver/anatomy & histology , Male , Mustard Plant , Myocardium/pathology , Oils/analysis , Organ Size , Phosphorus/metabolism , Plants, Medicinal , Structure-Activity Relationship , Time FactorsSubject(s)
Aflatoxins/adverse effects , Liver Regeneration/drug effects , Mitosis/drug effects , Animals , Hepatectomy , Liver/pathology , Male , RatsABSTRACT
PIP: The effects of 2 oral contraceptives, Ovulen and Norlestrin, were studied in monkeys fed adequate protein and low protein diets. The experiment was carried out in parts. In the first one, the administration of contraceptives was cyclic and similar to that employed in human subjects. In the other experiments, the contraceptives were given continuously and an attempt was made to exaggerate the deleterious effects of the oral contraceptive on the liver by including small doses of a known hepatotoxic agent, aflatoxin (AT). In Experiment 1, 45 female monkeys were divided into 2 groups of 20 and 25 and received an adequate protein (16%) and low protein diet (4%) respectively. Each monkey was fed 1/5 of a tablet of Ovulen or Norlestrin orally for 3 weeks, and then administration was discontinued for 1 week. In Experiment 2, 35 female monkeys were divided into 7 groups of 5 each. All the animals recieved 4% protein diet. 5 groups were tube fed at the rate of 100 cal/kg body weight, while 2 groups were given diet ad libitum. Group I received the diet alone while groups II-V received 10 mcg AT, 25 mcg AT, 10 mcg AT plus 1/5 Ovulen tablets, and 25 mcg AT plus 1/5 Ovulen tablet respectively daily. Groups VI and VII received the diet ad libitum but were orally fed 75 mcg AT and 75 mcg AT plus 1/5 Ovulen tablet respectively. Serum glutamic-oxalacetic transaminase activity and alkaline phosphatase activity were studied at regular intervals after the administation of oral contraceptives in the experiments. Serum proteins and hemoglobin were also determined. Monkeys fed oral contraceptives showed increased serum glutamic-oxalacetic transaminase and alkaline phosphatase activities irrespective of the level of protein in the diet. Livers of animals receiving oral contraceptives were morphologically similar to the controls fed respective diets. The experiments were conducted for a period of almost 2 years.^ieng
