Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 25
Filter
Add more filters










Publication year range
1.
Int J Food Microbiol ; 116(1): 82-7, 2007 May 01.
Article in English | MEDLINE | ID: mdl-17300847

ABSTRACT

One hundred and seventy-six Enterococcus faecium isolates from Slovak dairy product Bryndza were tested for the presence of plasmid DNA. Eighty-two isolates were positive and their plasmid DNA was isolated and digested by EcoRI and HindIII restriction endonucleases. The patterns obtained were compared with those obtained after pulsed-field gel electrophoresis of macrorestriction fragments (PFGE), (GTG)(5)-PCR and ERIC-PCR. All these molecular approaches were applied for the study of genetic variability and determination of strain relatednesses among plasmid-positive isolates of E. faecium. In general, all methods revealed a considerable genetic diversity of E. faecium isolates. Plasmid profiling and ERIC-PCR have offered a higher resolution than PFGE and (GTG)(5)-PCR.


Subject(s)
Cheese/microbiology , DNA, Bacterial/analysis , Enterococcus faecium/genetics , Food Microbiology , Genetic Variation , Electrophoresis, Gel, Pulsed-Field , Enterococcus faecium/classification , Enterococcus faecium/isolation & purification , Plasmids , Polymerase Chain Reaction , Restriction Mapping
2.
Lett Appl Microbiol ; 42(6): 553-9, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16706891

ABSTRACT

AIMS: To identify enterococci isolated from sheep milk cheese--bryndza, and to compare differences in the composition of enterococcal microflora affected by the season, and to evaluate the potential presence of vancomycin resistance and virulence determinants. METHODS AND RESULTS: Bacterial strains were isolated during analysis of bryndza cheese and identified on the genus and species level by phenotypic methods and with commercial biochemical sets. The identification of the species, Enterococcus faecium, Ent. durans and Ent. faecalis, was confirmed by PCR using species-specific primers for ddl genes. PCR was also used for assessment of presence of vanA and vanB genes and virulence determinants gelE, agg and cytolysin genes namely: cylL(L), cylL(S), cylM, cylB and cylA. Among 308 Enterococcus sp. strains, 177 isolates were proved to be Ent. faecium, 59 to be Ent. durans and 41 to be Ent. faecalis. Vancomycin resistance genes vanA and vanB were not detected. Agar plate testing confirmed their absence. Gene gelE, however, was found in 20 Ent. faecalis isolates, but only 13 of them showed gelatinase-positive phenotype. Seven isolates had five cytolysin genes, but none of the isolates exhibited a positive haemolytic phenotype. Four isolates possessed the agg gene. The prevalence of Ent. faecium species was highest in samples from the winter season harvest. CONCLUSIONS: Ent. faecium is the dominant enterococcal species in bryndza cheese and the most prevalent in the winter season product. None of the Enterococcus sp. strains was proved to have vanA or vanB genes and the vancomycin resistance. SIGNIFICANCE AND IMPACT OF THE STUDY: To our knowledge, this is the first report of enterococcal microflora in bryndza cheese and its evaluation for the presence of vanA and vanB genes as well as virulence determinants.


Subject(s)
Cheese/microbiology , Enterococcus/classification , Enterococcus/pathogenicity , Vancomycin Resistance/genetics , Virulence Factors/genetics , Animals , Bacterial Proteins/genetics , Carbon-Oxygen Ligases/genetics , Enterococcus/drug effects , Enterococcus/isolation & purification , Enterococcus faecalis/classification , Enterococcus faecalis/drug effects , Enterococcus faecalis/isolation & purification , Enterococcus faecalis/pathogenicity , Milk/chemistry , Polymerase Chain Reaction , Seasons , Sheep , Virulence
3.
Folia Microbiol (Praha) ; 49(3): 301-5, 2004.
Article in English | MEDLINE | ID: mdl-15259772

ABSTRACT

Concentrated extracts of MRS (De Man-Rogosa-Sharpe) media in which probiotic bacterium Enterococcus faecium strain M-74 was grown exerted different antimutagenic activity against ofloxacin-, N-methyl, N'-nitro-N-nitrosoguanidine- and sodium 5-nitro-2-furylacrylate-induced mutagenicity in Salmonella typhimurium assay depending on the presence (+Se) or absence of disodium selenite pentahydrate (-Se). The antimutagenicity of MRS(+Se) extract was higher than that of MRS(-Se) extract. Selenium enhanced also the antimutagenic effect of both live and killed cells of E. faecium M-74, respectively. The live bacteria decreased the mutagenicity of selected substances more than killed cells. Synergic activity of selenium with the bacterium was also manifested.


Subject(s)
Enterococcus faecium/physiology , Mutagenesis/drug effects , Mutagens/toxicity , Probiotics/pharmacology , Salmonella typhimurium/drug effects , Selenium/pharmacology , Antimutagenic Agents/pharmacology , Drug Synergism , Enterococcus faecium/metabolism , Mutagenicity Tests , Salmonella typhimurium/genetics
4.
Phytomedicine ; 11(7-8): 673-8, 2004 Nov.
Article in English | MEDLINE | ID: mdl-15636184

ABSTRACT

Saponins, steroid or triterpene glycosides, are known to have a broad spectrum of biological and pharmacological activities. Three different triterpenoid saponins, marked here as 1s, 2s and 3s, from involucral bracts of Cynara cardunculus L. were isolated and their antimutagenic effect was assessed. Using spectrophotometric method it was shown that all three substances, 1s, 2s and 3s, possess very good absorptive capability. The antimutagenic effect of these substances was estimated against acridine orange (AO)- and ofloxacin-induced damage of chloroplast DNA in Euglena gracilis assay. These cynarasaponins were experimentally confirmed to exhibit different, statistically significant activity in reducing damage of chloroplast DNA of the flagellate E. gracilis induced by AO and ofloxacin (p(t) < 0.05-0.01). Our findings suggest that the antimutagenic effect of 1s, 2s and 3s against AO-induced chloroplast DNA impairment could be a result of their absorptive capacity. As far as ofloxacin is concerned, a possible mechanism of the reduction of the chloroplast DNA lesion was not elucidated so far. To our knowledge, these data demonstrate for the first time the antimutagnic activity of saponins isolated from involucral bracts of C. cardunculus exerted through different mode of action.


Subject(s)
Antimutagenic Agents/pharmacology , Cynara/chemistry , Saponins/pharmacology , Triterpenes/pharmacology , Acridine Orange/toxicity , Animals , Antimutagenic Agents/isolation & purification , Euglena gracilis/drug effects , Euglena gracilis/genetics , Molecular Structure , Ofloxacin/antagonists & inhibitors , Ofloxacin/toxicity , Saponins/isolation & purification , Triterpenes/isolation & purification
5.
Mutat Res ; 497(1-2): 213-22, 2001 Oct 18.
Article in English | MEDLINE | ID: mdl-11525924

ABSTRACT

Antioxidative and antimutagenic effect of yeast cell wall mannans, in particular, extracellular glucomannan (EC-GM) and glucomannan (GM-C.u.) both from Candida utilis, mannan from Saccharomyces cerevisiae (M-S.c.) and mannan from Candida albicans (M-C.a.) was evaluated. Luminol-dependent photochemical method using trolox as a standard showed that EC-GM, GM-C.u., M-S.c. and M-C.a. have relatively good antioxidative properties. EC-GM exhibited the highest antioxidative activity, followed by GM-C.u. and M-S.c. M-C.a. showed the least antioxidative activity. These mannans were experimentally confirmed to exhibit different, statistically significant antimutagenic activity in reducing damage of chloroplast DNA of the flagellate Euglena gracilis induced by ofloxacin and acridine orange (AO). We suggest that the antimutagenic effect of EC-GM, GM-C.u., M-S.c. and M-C.a. against ofloxacin is based on their ability to scavenge reactive oxygen radicals. With AO, the reduction of the chloroplast DNA lession could be a result of the absorptive capacity of the mannans. The important characteristics of mannans isolated from the yeast cell walls, such as good water solubility, relatively small molecular weight (15-30kDa), and antimutagenic effect exerted through different mode of action, appear to be a promising features for their prospective use as a natural protective (antimutagenic) agents.


Subject(s)
Antimutagenic Agents/pharmacology , Antioxidants/pharmacology , Mannans/pharmacology , Acridine Orange/toxicity , Animals , Candida/chemistry , Candida albicans/chemistry , Cell Wall/chemistry , DNA Damage , DNA, Chloroplast/drug effects , Euglena gracilis/drug effects , In Vitro Techniques , Luminol , Mannans/isolation & purification , Mutagenicity Tests , Ofloxacin/toxicity , Photochemistry , Saccharomyces cerevisiae/chemistry , Spectroscopy, Fourier Transform Infrared
6.
Folia Microbiol (Praha) ; 46(6): 511-4, 2001.
Article in English | MEDLINE | ID: mdl-11898340

ABSTRACT

Naturally occurring plant phenolics, p-coumaric acid (PA), caffeic acid (CA), ferulic acid (FA) and gentisic acid (GA) (25-100 nmol/L) had protective effects on acridine orange (AO; 216 mumol/L)- and ofloxacin (3 mumol/L)-induced genotoxicity in Salmonella typhimurium. FA, GA and CA exhibited a significant concentration-dependent protective effect against the genotoxicity of AO and ofloxacin, with the exception of PA, which at all concentrations tested abolished the AO and ofloxacin genotoxicity. UV spectrophotometric measurements showed the interaction of PA, FA, GA and CA with AO but not with ofloxacin; this interaction is obviously responsible for the reduction of AO-induced S. typhimurium mutagenicity. In the case of ofloxacin the antimutagenic effect of PA, FA, GA and CA is assumed to be a result of their ability to scavenge reactive oxygen species (ROS) produced by ofloxacin.


Subject(s)
Acridine Orange/toxicity , Antimutagenic Agents/pharmacology , Gentisates , Hydroxybenzoates/pharmacology , Mutagens/toxicity , Ofloxacin/toxicity , Salmonella typhimurium/drug effects , Caffeic Acids/pharmacology , Coumaric Acids/pharmacology , Dose-Response Relationship, Drug , Mutagenicity Tests , Propionates , Salmonella typhimurium/genetics
7.
Mutat Res ; 469(1): 107-14, 2000 Aug 21.
Article in English | MEDLINE | ID: mdl-10946247

ABSTRACT

The mutagenicity (bleaching activity) of ofloxacin (43 microM) and acridine orange (AO) (13.5 microM) in Euglena gracilis is inhibited by plant phenolics. Caffeic acid (CA), p-coumaric acid (PCA), ferulic acid (FA) and gentisic acid (GA) (25, 50, 100 and 250 microM) exhibited a significant concentration-dependent inhibitory effect against ofloxacin-induced mutagenicity, which was very effectively eliminated by the highest concentration of all four of those phenolic acids. The mutagenicity of AO was also significantly reduced in the presence of CA, PCA and FA (25, 50, 100 and 250 microM). However, GA exhibited no significant activity, even at the concentration of 250 microM. Based on the UV spectrophotometric measurements, we suggest that the antimutagenic effect of CA, PCA, FA and GA resulted from the scavenging of reactive oxygen species (ROS) produced by ofloxacin. On the other hand, the reduction of AO-induced mutagenicity correlates with the binding capabilities of CA, PCA and FA, with the exception of GA.


Subject(s)
Acridine Orange/toxicity , Antimutagenic Agents/pharmacology , Chloroplasts/drug effects , Euglena gracilis/genetics , Gentisates , Hydroxybenzoates/pharmacology , Mutagens/toxicity , Ofloxacin/toxicity , Animals , Antioxidants/pharmacology , Caffeic Acids/pharmacology , Chloroplasts/genetics , Coumaric Acids/pharmacology , Euglena gracilis/drug effects , Mutagenicity Tests , Ofloxacin/antagonists & inhibitors , Propionates
8.
Anticancer Res ; 20(2A): 833-6, 2000.
Article in English | MEDLINE | ID: mdl-10810362

ABSTRACT

The possible protective effect of sulphur-free beech lignin polymer on the mutagenicity of ofloxacin in Euglena gracilis was studied. The generation of oxygen species by ofloxacin and their possible interaction with lignin was verified by physico-chemical measurements. The UV absorbance spectra of ofloxacin with and without lignin showed no interaction between these two compounds. The production of superoxide anion radical (O2-) by ofloxacin was significantly reduced in the presence of lignin (AIR = 0.57 +/- 0.03, p < 0.01). Lignin, at concentrations of 125 and 250 micrograms/ml decreased the E. gracilis bleaching activity of ofloxacin to 39.9% and 2.8%, respectively. A lignin concentration of 500 micrograms/ml eliminated the bleaching activity of ofloxacin very efficiently. Our results are consistent with the concept that lignin biopolymer has the capability of reducing genotoxic activity by scavenging reactive oxygen species.


Subject(s)
Antimutagenic Agents/pharmacology , Euglena gracilis/drug effects , Lignin/pharmacology , Ofloxacin/toxicity , Animals , Euglena gracilis/physiology , Free Radical Scavengers , Mutagenicity Tests , Superoxides/metabolism
9.
Anticancer Res ; 19(1A): 569-72, 1999.
Article in English | MEDLINE | ID: mdl-10226600

ABSTRACT

The inhibitory activity of lignin against nitrosoguanidine (MNNG)- and acridine orange (AO)- induced mutagenesis was examined using two microbial systems: green unicellular flagellate Euglena gracilis and Salmonella typhimurium TA100 and TA97. To verify the hypothesis that the above mentioned mutagens may generate some oxidant species and subsequently free radicals, or they may interact with lignin, two physico-chemical measurements were performed. Lignin at a tested concentration (100 micrograms/ml) decreases Euglena-bleaching activity of MNNG by 67.7% and AO by 99.7%. Percentage of MNNG-induced revertants of S. typhimurium was also decreased substantially by lignin. We conclude that our results indicate the possible mechanisms behind the antimutagenic/anticarcinogenic effects of lignin: namely, scavening of reactive oxygen species produced by MNNG and binding of AO itself.


Subject(s)
Antimutagenic Agents/pharmacology , Lignin/pharmacology , Acridine Orange/toxicity , Animals , Euglena gracilis/drug effects , Methylnitronitrosoguanidine/toxicity , Salmonella typhimurium/drug effects , Superoxides/metabolism
10.
Mutat Res ; 446(2): 225-30, 1999 Dec 13.
Article in English | MEDLINE | ID: mdl-10635345

ABSTRACT

The possible protective effect of a suberin extract from Quercus suber cork on acridine orange (AO)-, ofloxacin- and UV radiation-induced mutagenicity (bleaching activity) in Euglena gracilis was examined. To our knowledge, the present results are the first attempt to analyse suberin in relation to mutagenicity of some chemicals. Suberin exhibits a significant dose-dependent protective effect against AO-induced mutagenicity and the concentration of 500 micrograms/ml completely eliminates the Euglena-bleaching activity of AO. The mutagenicity of ofloxacin is also significantly reduced in the presence of suberin (125, 250 and 500 micrograms/ml). However, the moderate protective effect of suberin on UV radiation-induced mutagenicity was observed only at concentrations 500 and 1000 micrograms/ml. Our data shows that suberin extract from Q. suber cork possess antimutagenic properties and can be included in the group of natural antimutagens acting in a desmutagenic manner.


Subject(s)
Antimutagenic Agents/pharmacology , Membrane Lipids/pharmacology , Mutagenicity Tests/methods , Plant Extracts/pharmacology , Trees/chemistry , Acridine Orange/toxicity , Animals , Euglena gracilis/drug effects , Euglena gracilis/genetics , Euglena gracilis/radiation effects , Lipids , Mutagens/toxicity , Ofloxacin/toxicity , Plant Shoots/chemistry , Spectrophotometry, Ultraviolet , Superoxides/metabolism , Ultraviolet Rays
11.
Mutat Res ; 416(1-2): 85-92, 1998 Aug 07.
Article in English | MEDLINE | ID: mdl-9725994

ABSTRACT

The antimutagenicity of 14 naturally occurring flavonoids (20 mumol/l) on ofloxacin (43 mumol/l and 86 mumol/l)-induced bleaching (mutagenicity) was studied in Euglena gracilis. The flavonoids chrysin, techtochrysin, chrysin-5-methylether galangin, galangin-5-methylether, pinocembrin and pinobanksin possess considerable antimutagenic properties against ofloxacin-induced bleaching of E. gracilis. Apigenin and isalpinin had only weak antimutagenic potency. Pinobanksin-5-methylether and pinobanksin-3-acetate showed very weak or no antimutagenic effect. However, kempferol, quercetin-3-methylether and quercetin-3,3'-dimethylether showed co-mutagenic or no antimutagenic effect depending on the concentration of ofloxacin. Two possible modes of action of the flavonoids on ofloxacin-induced bleaching of E. gracilis are discussed.


Subject(s)
Antimutagenic Agents/pharmacology , Euglena gracilis/drug effects , Euglena gracilis/genetics , Flavanones , Flavonoids/pharmacology , Mutagens/toxicity , Ofloxacin/toxicity , Animals , Antimutagenic Agents/chemistry , Chloroplasts/drug effects , Flavonoids/chemistry
12.
Plant J ; 16(6): 673-80, 1998 Dec.
Article in English | MEDLINE | ID: mdl-10069074

ABSTRACT

Plant transformation via Agrobacterium frequently results in formation of multiple copy T-DNA arrays at one target site of the chromosome. The T-DNA copies are arranged in repeats, direct or inverted around one of the T-DNA borders. A Ti plasmid-derived transformation vector has been constructed enabling direct selection of transformants carrying at least two linked copies of T-DNA in the same orientation. The selection is based on expression of a promoterless neomycin phosphotransferase gene on one T-DNA copy from a promoter located on the other T-DNA copy. After co-cultivation of tobacco protoplasts with Agrobacterium, as many as 30% of regenerated transformed plants carried directly repeated T-DNA copies. The junction regions between two T-DNAs were amplified and 13 amplified fragments were cloned and sequenced. The involvement of T-DNA left and right border sequences in direct repeat junctions was determined. In some junctions, additional filler DNA was detected. The length of filler DNA varied from a few up to almost 300 bp. The longer filler DNAs from two clones were found to be T-DNA fragments in direct or reverse orientation. We discuss the recently suggested models for T-DNA integration and propose that the formation of direct repeats in genomes does not necessarily result from ligation of intermediates (i.e. T-strands), but more likely from the co-integration of several intermediates into one target site.


Subject(s)
DNA, Bacterial/genetics , Nicotiana/genetics , Plants, Toxic , Rhizobium/genetics , Base Sequence , DNA, Bacterial/chemistry , DNA, Plant/genetics , DNA, Single-Stranded/genetics , Models, Genetic , Nucleic Acid Conformation , Repetitive Sequences, Nucleic Acid , Transformation, Genetic
13.
Mutat Res ; 359(2): 85-93, 1996 Feb 29.
Article in English | MEDLINE | ID: mdl-8598835

ABSTRACT

The genotoxic effect of ofloxacin was significantly decreased by standard antimutagens (sodium selenite, ascorbic acid, butylated hydroxyanisole and butylated hydroxytoluene) in the unicellular flagellate Euglena gracilis. The antiofloxacin activity of sodium selenite was also documented by a bacterial test in which the repair-proficient strain Salmonella typhimurium TA102 was used.


Subject(s)
Anti-Infective Agents/toxicity , Antimutagenic Agents/pharmacology , Euglena gracilis/drug effects , Ofloxacin/toxicity , Animals , Ascorbic Acid/pharmacology , Butylated Hydroxyanisole/pharmacology , Butylated Hydroxytoluene/pharmacology , Dose-Response Relationship, Drug , Sodium Selenite/pharmacology
14.
Biochem J ; 305 ( Pt 2): 583-90, 1995 Jan 15.
Article in English | MEDLINE | ID: mdl-7832777

ABSTRACT

Pulmonary surfactant, a lipoprotein complex, is essential for normal lung function, and deficiency of surfactant can result in respiratory-distress syndrome (RDS) in the prematurely born infant. Some studies have pointed towards a genetic contribution to the aetiology of RDS. Because the surfactant protein B (SP-B) is important for optimal surfactant function and because it is involved in the pathogenesis of pulmonary disease, we investigated the genetic variability of the SP-B gene in individuals with and without RDS. We identified a 2.5 kb BamHI polymorphism and studied its location, nature and frequency. We localized this polymorphism in the first half of intron 4 and found that it is derived by gain or loss in the number of copies of a motif that consists of two elements, a 20 bp conserved sequence and a variable number of CA dinucleotides. Variability in the number of motifs resulting from either deletion (in 55.3% of the cases with the variation) or insertion (44.7%) of motifs was observed in genomic DNAs from unrelated individuals. Analysis of 219 genomic DNAs from infants with (n = 82) and without (n = 137) RDS showed that this insertion/deletion appears with significantly higher frequency in the RDS population (29.3 as against 16.8%, P < 0.05).


Subject(s)
Genetic Variation , Proteolipids/genetics , Pulmonary Surfactants/genetics , Repetitive Sequences, Nucleic Acid/genetics , Respiratory Distress Syndrome, Newborn/genetics , Base Sequence , Black People , Cloning, Molecular , Deoxyribonuclease BamHI , Gene Frequency , Genome, Human , Humans , Infant , Infant, Newborn , Introns/genetics , Molecular Sequence Data , Mutagenesis, Insertional , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Pulmonary Surfactants/deficiency , Sequence Analysis, DNA , Sequence Deletion , Sequence Homology, Nucleic Acid , White People
15.
Am J Physiol ; 266(5 Pt 1): L519-27, 1994 May.
Article in English | MEDLINE | ID: mdl-8203545

ABSTRACT

We have previously characterized two surfactant protein A (SP-A) cDNAs termed 1A and 6A, as well as a 6A allelic variant termed 6A1. These sequences are quite heterogeneous at the 3' untranslated region (3'UT). Differences between 6A and 6A1 alleles include an 11-bp insertion/deletion 407 bases downstream from the start of the translation termination codon and a base pair polymorphism (C or G) in exon 1 (position 1,193; White, Damm, Miller, Spratt, Schilling, Hawgood, Benson, and Cordell. Nature Lond. 317: 361-363, 1985). The 11-bp (GCCCACTGCCT) segment is present in 6A1 and absent in 6A. The 6A/6A genotype, in a small number of specimens, showed a trend toward a higher frequency in the black Nigerian population compared with Caucasians. In this report, we examine the frequency of the 6A genotype in a larger number of samples from Caucasians and black Nigerians as well as the meiotic stability of the 3'UT heterogeneity. Slot-blot analysis and allele-specific oligonucleotide probes have confirmed that the 6A/6A genotype is more frequent in the Nigerian population. Single-strand conformation polymorphisms in the 3'UT appear to be stably inherited by members of a three-generation family, suggesting that these nucleotide variants represent natural polymorphisms in the population.


Subject(s)
Gene Frequency , Proteolipids/genetics , Pulmonary Surfactants/genetics , Base Sequence , Black People/genetics , DNA/blood , DNA/genetics , DNA/isolation & purification , DNA Primers , Female , Genetic Linkage , Genetic Variation , Genotype , Glycoproteins/genetics , Humans , Male , Meiosis , Molecular Sequence Data , Nigeria , Oligodeoxyribonucleotides , Pedigree , Polymerase Chain Reaction/methods , Polymorphism, Genetic , Proteolipids/biosynthesis , Pulmonary Surfactant-Associated Protein A , Pulmonary Surfactant-Associated Proteins , Pulmonary Surfactants/biosynthesis , White People/genetics
16.
Folia Microbiol (Praha) ; 37(4): 273-8, 1992.
Article in English | MEDLINE | ID: mdl-1452097

ABSTRACT

A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.


Subject(s)
Ovalbumin/genetics , Saccharomyces cerevisiae/genetics , Animals , Coturnix , DNA/genetics , Gene Expression/genetics , Genetic Vectors , Ovalbumin/biosynthesis , Plasmids , Recombinant Proteins/biosynthesis
17.
Folia Microbiol (Praha) ; 36(4): 406-7, 1991.
Article in English | MEDLINE | ID: mdl-1822844

ABSTRACT

A method for the extraction of genomic DNA from the industrial yeast Candida utilis is described. The method is rapid, simple and produces DNA that is sufficiently pure for restriction analysis and should be suitable for Southern blotting and the construction of gene libraries.


Subject(s)
Candida/genetics , DNA, Fungal/isolation & purification , Genetic Techniques
18.
Folia Microbiol (Praha) ; 24(2): 171-5, 1979.
Article in English | MEDLINE | ID: mdl-572318

ABSTRACT

Study of nematocide effect of 48 species of entomophilic and entomophagous fungi from the class Fungi imperfecti against Panagrellus redivivus and Rhabditis oxycerca nematodes revealed a high frequency of fungi with nematocide effect. A close correlation between these two effects was disclosed by measuring the intensity of the nematocide and insecticide effect of fungal extracts.


Subject(s)
Antinematodal Agents/metabolism , Mitosporic Fungi/metabolism , Animals , Antinematodal Agents/pharmacology , Nematoda/drug effects
19.
J Antibiot (Tokyo) ; 29(9): 954-7, 1976 Sep.
Article in English | MEDLINE | ID: mdl-993135

ABSTRACT

From soil samples collected in the jungles of Ninh Binh Province of the Vietnamese Democratic Republic, 97 isolates belonging to 54 species of Fungi imperfecti were obtained and screened for the production of antibiotics. The isolates were identified as belonging to the orders Melanconiales, Moniliales, Sphaeropsidales and Mycelia sterilia. In the species studied a very high incidence of antiprotozoal activities were observed.


Subject(s)
Anti-Bacterial Agents , Mitosporic Fungi/metabolism , Animals , Anti-Bacterial Agents/biosynthesis , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Drug Evaluation, Preclinical , Eukaryota/drug effects , Fungi/drug effects , Nematoda/drug effects , Vietnam
20.
Folia Microbiol (Praha) ; 21(6): 493-4, 1976.
Article in English | MEDLINE | ID: mdl-1033116

ABSTRACT

Dactylaria pyriformis Juniper and Dactylaria thaumasia Drechsler are predacious fung forming three-edimensional sticky reticula in which nematodes are captured. It was shown by methods developed in our laboratory that in submerged cultivations both of these fungi produce substances attracting nematodes and compounds having nematicidal activity.


Subject(s)
Anti-Bacterial Agents/biosynthesis , Antinematodal Agents , Chemotaxis , Mitosporic Fungi/metabolism , Nematoda/physiology , Anti-Bacterial Agents/pharmacology , Ecology , Nematoda/drug effects
SELECTION OF CITATIONS
SEARCH DETAIL
...