Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 32
Filter
Add more filters










Publication year range
1.
Am J Physiol Heart Circ Physiol ; 291(6): H2987-96, 2006 Dec.
Article in English | MEDLINE | ID: mdl-16844912

ABSTRACT

Dietary flaxseed has significant anti-atherogenic effects. However, the limits of this action and its effects on vascular contractile function are not known. We evaluated the effects of flaxseed supplementation on atherosclerosis and vascular function under prolonged hypercholesterolemic conditions in New Zealand White rabbits assigned to one of four groups for 6, 8, or 16 wk of feeding: regular diet (RG), 10% flaxseed-supplemented diet (FX), 0.5% cholesterol-supplemented diet (CH), and 0.5% cholesterol- and 10% flaxseed-supplemented diet (CF). Cholesterol feeding resulted in elevated plasma cholesterol levels and the development of atherosclerosis. The CF group had significantly less atherosclerotic lesions in the aorta and carotid arteries after 6 and 8 wk than the CH animals. However, the anti-atherogenic effect of flaxseed supplementation was completely attenuated by 16 wk. Maximal tension induced in aortic rings either by KCl or norepinephrine was not impaired by dietary cholesterol until 16 wk. This functional impairment was not prevented by including flaxseed in the high-cholesterol diet. Aortic rings from the cholesterol-fed rabbits exhibited an impaired relaxation response to acetylcholine at all time points examined. Including flaxseed in the high-cholesterol diet completely normalized the relaxation response at 6 and 8 wk and partially restored it at 16 wk. No significant changes in the relaxation response induced by sodium nitroprusside were observed in any of the groups. In summary, dietary flaxseed is a valuable strategy to limit cholesterol-induced atherogenesis as well as abnormalities in endothelial-dependent vasorelaxation. However, these beneficial effects were attenuated during prolonged hypercholesterolemic conditions.


Subject(s)
Coronary Artery Disease/diet therapy , Coronary Artery Disease/physiopathology , Dietary Supplements , Flax , Hypercholesterolemia/physiopathology , Vasoconstriction/drug effects , Animals , Aorta/pathology , Aorta/physiopathology , Carotid Arteries/pathology , Carotid Arteries/physiopathology , Cholesterol/blood , Cholesterol, Dietary/pharmacology , Coronary Artery Disease/pathology , Fatty Acids/blood , Hypercholesterolemia/pathology , Male , Nitroprusside/pharmacology , Rabbits , Triglycerides/blood , Vasoconstriction/physiology , Vasodilator Agents/pharmacology
2.
J Lipid Res ; 42(5): 716-24, 2001 May.
Article in English | MEDLINE | ID: mdl-11352978

ABSTRACT

Cytosolic phospholipase A(2) (cPLA(2)) is responsible for the release of arachidonic acid, a precursor for eicosanoid biosynthesis, from cellular phospholipids. The objective of this study is to examine the regulation of cPLA(2) by peroxisome proliferator-activated receptor (PPAR) activators in preadipocyte SW872 (SW) cells. PPAR belong to the superfamily of nuclear hormone receptors that heterodimerize with the retinoid X receptor. In this study, the presence of both PPARalpha and PPARgamma was confirmed in SW cells by positive identification of their mRNA in the cellular homogenate. Clofibrate, a PPARalpha activator, caused an enhancement of ionophore A-23187-induced arachidonate release in SW cells. This increase resulted from an enhancement of cPLA(2) activity, which was caused by an increase in enzyme protein. Clofibrate at lower concentrations (10-200 microM) produced increases in the mRNA levels of cPLA(2) in a dose-response manner. At higher concentrations (>400 microM), clofibrate treatment resulted in the attenuation of the cPLA(2) mRNA level and protein expression. We postulate that clofibrate, acting through the PPARalpha, caused an induction in the transcription of cPLA(2) gene, which led to an increase in the cPLA(2) protein. The observed increase in arachidonate release in SW cells appeared to be a direct result of the enhanced cPLA(2) activity.


Subject(s)
Adipocytes/drug effects , Clofibrate/pharmacology , Phospholipases A/metabolism , Receptors, Cytoplasmic and Nuclear/metabolism , Transcription Factors/metabolism , Acyl-CoA Oxidase , Adipocytes/enzymology , Arachidonic Acid/biosynthesis , Arachidonic Acid/metabolism , Calcimycin/pharmacology , Cyclooxygenase 2 , Enzyme Activation , Humans , Hypolipidemic Agents/pharmacology , Ionophores/pharmacology , Isoenzymes/genetics , Isoenzymes/metabolism , Liposarcoma , Membrane Proteins , Oxidoreductases/genetics , Oxidoreductases/metabolism , Phospholipases A/genetics , Prostaglandin-Endoperoxide Synthases/genetics , Prostaglandin-Endoperoxide Synthases/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Cytoplasmic and Nuclear/agonists , Receptors, Cytoplasmic and Nuclear/genetics , Reverse Transcriptase Polymerase Chain Reaction , Transcription Factors/agonists , Transcription Factors/genetics , Tumor Cells, Cultured , Up-Regulation
3.
Mol Cell Biochem ; 207(1-2): 9-17, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10888221

ABSTRACT

Atorvastatin is an established HMG-CoA reductase inhibitor which effectively reduces the plasma low density lipoprotein (LDL)-cholesterol level in hyperlipidemic patients. The present study was designed to investigate whether atorvastatin treatment can modify the biochemical content of oxidized LDL in hyperlipidemic patients and the ability of oxidized LDL to impair the endothelium-dependent relaxation of blood vessels. With atorvastatin (10 mg/day) treatment for 4 weeks in 19 type IIa hyperlipidemic patients, total cholesterol level was lowered by 23%, LDL-cholesterol was lowered by 32% and triacylglycerol was lowered by 19% as compared with dietary therapy alone. High density lipoprotein levels increased by approximately 9%. The ability of oxidized LDL from hyperlipidemic patients after atorvastatin treatment to impair the endothelium-dependent relaxation was significantly reduced as compared with dietary intervention alone. Analysis of the biochemical contents of oxidized LDL from this group revealed that there was an 11% reduction in lysophosphatidylcholine (LPC) as compared with the group that received only dietary counseling. A decrease in the C16:0 moiety with a corresponding increase in the C18:0 moiety of LPC in the oxidized LDL was also observed in the atorvastatin treated group. We propose that the observed reduction and the change in composition of acyl groups in LPC in the oxidized LDL of the atorvastatin-treated group results from a combination of the continued dietary treatment as well as drug therapy. In view of an observation that both C16:0 and C18:0 LPC species are equally potent in the impairment of endothelium-dependent relaxation of the aortic rings, we feel that the reduced level of LPC in the oxidized LDL produced by atorvastatin treatment is partially responsible for the improvement in endothelium control of vascular tone.


Subject(s)
Anticholesteremic Agents/therapeutic use , Heptanoic Acids/therapeutic use , Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Hyperlipoproteinemia Type II/drug therapy , Lipoproteins, LDL/blood , Pyrroles/therapeutic use , Adult , Atorvastatin , Combined Modality Therapy , Diet Therapy , Endothelium, Vascular/drug effects , Humans , Hyperlipoproteinemia Type II/blood , Lysophosphatidylcholines/blood , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/physiology , Oxidation-Reduction , Phospholipids/blood
4.
Mol Cell Biochem ; 207(1-2): 123-9, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10888237

ABSTRACT

The objective of the research project was to investigate whether fenofibrate treatment may alter the biochemical content of the oxidized LDL and consequently its ability to impair the endothelium-dependent relaxation in hyperlipidemic patients. We hypothesized that fenofibrate treatment of hyperlipidemic patients may attenuate the ability of their oxidized LDL to impair the endothelium-dependent relaxation of the blood vessels as a consequence of fenofibrate-induced changes to the content and composition of lysoPC in the LDL molecule. Hyperlipidemic patients (Type IIb and Type IV) were recruited from the Lipid Clinic, HSC, Winnipeg, Canada, for this study. A blood sample was taken immediately after the recruitment, a second sample was taken after 6 weeks of dietary treatment, and a third sample was taken after 8 weeks of fenofibrate treatment. LDL was isolated from the plasma and oxidized by copper sulfate. Fenofibrate was shown to be highly effect in the reduction of total cholesterol, LDL cholesterol and triglycerides in these patients. Fenofibrate treatment also caused the attenuation of impairment of endothelium-dependent relaxation by the oxidized LDL from these patients. A slight reduction of lysophosphatidylcholine level was also found in the oxidized LDL of the fenofibrate treated patients, relative to LDL isolated after dietary treatment. In addition there were no changes in the fatty acid levels of the lysophosphatidylcholine isolated from LDL. Taken together, our results suggest that while the reduced lysophosphatidylcholine levels may contribute to the attenuated impairment of the endothelium-dependent relaxation of the aortic ring, other unidentified factors impacted by fenofibrate are likely to contribute to the attenuated effects.


Subject(s)
Endothelium, Vascular/physiology , Fenofibrate/therapeutic use , Hyperlipidemias/drug therapy , Hypolipidemic Agents/therapeutic use , Lipoproteins, LDL/blood , Muscle, Smooth, Vascular/physiology , Vasodilation/physiology , Acetylcholine/metabolism , Animals , Aorta, Thoracic , Cholesterol/blood , Cholesterol, LDL/blood , Chromatography, Gas/methods , Humans , Hyperlipidemias/metabolism , Lipids/analysis , Lipids/blood , Lysophosphatidylcholines/metabolism , Oxidation-Reduction , Rats , Triglycerides/blood
5.
Mol Cell Biochem ; 197(1-2): 1-6, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10485317

ABSTRACT

Hyperlipidemia has been associated with an increase in the incidence of atherosclerosis. The oxidation of low density lipoprotein (LDL) plays an important role in the initiation and progression of atherosclerosis, one of its effects being the inhibition of endothelium dependent relaxation (EDR). The elevated level of lysophosphatidylcholine (LPC) in oxidatively modified LDL has been shown to be a biochemical factor responsible for the impairment of EDR in vascular ring preparations. Several endothelium-derived modulators are thought to control vascular responsiveness. The present work examined whether acetylcholine (ACh)-induced EDR in rat aorta (pre-contracted with phenylephrine, PE) involved both endothelium-derived nitric oxide (EDNO) and endothelium-dependent hyperpolarizing factor (EDHF) and whether LPC inhibited either of these selectively. Indomethacin (10(-5) M), had no significant effect on EDR, indicating that products of cyclooxygenase, including prostacyclin, are not involved. Treatment with either N(W)-nitro-L-arginine methyl ester (L-NAME, 6.8 microM) to inhibit the production of EDNO or with elevated K+ (15 mM), to block the hyperpolarizing effect of EDHF impaired EDR considerably (each of these shifting the inhibitory dose-response relationship to ACh by almost one log unit); in muscles treated with both of these agents EDR was completely inhibited. In each of L-NAME- and K-treated muscles, the addition of LPC (20 microM) further impaired EDR. LPC did not independently raise the tone of resting- or PE-contracted aorta. We conclude that the inhibition of EDR of rat aorta by LPC involves the actions of both EDNO and EDHF.


Subject(s)
Biological Factors/physiology , Endothelium, Vascular/drug effects , Lysophosphatidylcholines/pharmacology , Nitric Oxide/physiology , Acetylcholine/pharmacology , Animals , Calcium/metabolism , Calcium Channels/metabolism , Endothelium, Vascular/metabolism , Endothelium, Vascular/physiology , In Vitro Techniques , Ion Transport , Muscle Relaxation/drug effects , Rats , Rats, Sprague-Dawley
6.
Biochim Biophys Acta ; 1347(2-3): 183-90, 1997 Aug 16.
Article in English | MEDLINE | ID: mdl-9295162

ABSTRACT

Phosphatidylcholine is the major phospholipid in mammalian tissues and the biosynthesis of phosphatidylcholine in H9c2 cells was previously shown to be stimulated by angiotensin II. In this study, we used the potent AT1 receptor antagonist, losartan, to determine if the angiotensin II-mediated stimulation of phosphatidylcholine biosynthesis was mediated by AT1 receptors. H9c2 cells were incubated with angiotensin II in the absence or presence of various concentrations of losartan. The cells were then incubated with [methyl-3H]choline for an additional 60 min and the radioactivity incorporated into phosphatidylcholine and its choline-containing metabolites determined. Losartan at concentrations which block AT1 receptors did not effect phosphatidylcholine biosynthesis mediated by angiotensin II. In contrast, higher concentrations of losartan inhibited radioactivity incorporated into phosphatidylcholine and its metabolites and this was due to a losartan-mediated reduction in choline uptake. Kinetic studies revealed that the losartan-mediated inhibition of choline uptake was competitive. High concentrations of losartan caused a translocation of CTP:phosphocholine cytidylyltransferase from the cytosolic (inactive) to the membrane (active) fraction likely as a compensatory mechanism for the losartan-mediated reduction in new phosphatidylcholine biosynthesis. Incubation of cells with PD123319, a potent AT2-receptor antagonist, did not block the angiotensin II-mediated stimulation of phosphatidylcholine biosynthesis. The results suggest that angiotensin II stimulates phosphatidylcholine biosynthesis independent of AT1- and AT2-receptor activation and losartan inhibits phosphatidylcholine biosynthesis by reducing choline uptake in H9c2 cells.


Subject(s)
Angiotensin Receptor Antagonists , Biphenyl Compounds/pharmacology , Imidazoles/pharmacology , Phosphatidylcholines/biosynthesis , Tetrazoles/pharmacology , Angiotensin II/pharmacology , Cell Line , Cell Membrane/metabolism , Choline/metabolism , Cytosol/metabolism , Immunoblotting , Kinetics , Losartan , Pyridines/pharmacology , Tritium
7.
J Lipid Res ; 38(3): 546-53, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9101435

ABSTRACT

The elevated level of plasma low density lipoprotein (LDL) in hyperlipidemic patients is an important risk factor for the production of atherosclerosis. Plasma LDL must be modified before it can produce an impairment of endothelium-dependent relaxation in aortic rings or enhancement of uptake by macrophages. The dramatic increase in lysophosphatidylcholine (lysoPC) content in oxidatively modified LDL has been touted as an important biochemical factor for the impairment of endothelium-dependent relaxation. The present study was designed to examine the lysoPC composition of oxidized LDL samples from normal and hyperlipidemic subjects, and their effects on the impairment of endothelium-dependent relaxation. Oxidatively modified LDL from hyperlipidemic patients contained a slightly higher level (17%) of lysoPC, but produced a disproportionately greater impairment of endothelium-dependent relaxation than that from normal subjects. As lysoPC is composed of many molecular species, its composition in oxidized LDL samples was analyzed. In hyperlipidemic patients, lysoPC samples were found to contain a higher proportion of long-chain acyl groups. Subsequent studies revealed that only long-chain lysoPC (C > 16:0) were effective in impairing endothelium-dependent relaxation. Experimental loading of oxidized LDL from normal subjects with long chain lysoPC to mimic levels observed in oxidized LDL from hyperlipidemic patients resulted in further impairment of endothelium-dependent relaxation. We conclude that the greater proportion of long-chain lysoPC found in the oxidized LDL of hyperlipidemic subjects is responsible for the increased impairment of endothelium-dependent vascular relaxation. We propose that the high level of LDL found in the plasma of hyperlipidemic patients, coupled with its enhanced ability to generate long chain species of lysoPC during oxidative modification, are important factors for the development of atherosclerosis in these patients.


Subject(s)
Endothelium, Vascular/physiology , Hyperlipidemias/metabolism , Lipoproteins, LDL/chemistry , Lysophosphatidylcholines/analysis , Muscle, Smooth, Vascular/physiology , Adult , Animals , Aorta , Female , Humans , In Vitro Techniques , Lipids/blood , Lipoproteins, LDL/metabolism , Lipoproteins, LDL/pharmacology , Male , Middle Aged , Muscle Relaxation/drug effects , Oxidation-Reduction , Rats
10.
Am J Physiol ; 258(1 Pt 2): H17-23, 1990 Jan.
Article in English | MEDLINE | ID: mdl-2301606

ABSTRACT

Perfusion of the rat right ventricular wall muscle for 4 min with a Ca2(+)-free medium followed by perfusion with a Ca2(+)-containing solution resulted in a 42% recovery of developed tension, contracture, and a massive release of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) from the muscle. High concentrations (1-5 mM) of amiloride partially protected the ventricular wall from Ca2+ paradox-induced dysfunction. The inclusion of benzamil, an amiloride analogue, 2 min before and during the Ca2(+)-free perfusion period prevented contracture development, restored force development, and almost totally eliminated the release of CPK and LDH from the muscle. Contractile function was best protected by 10-50 microM benzamil. The results demonstrate the efficacy of benzamil as a protective agent against Ca2+ paradox-induced myocardial dysfunction and damage. In view of the known capacity of benzamil to block transsarcolemmal Na(+)-Ca2+ exchange, this study supports the involvement of elevated intracellular Na+ and a stimulation of Na(+)-Ca2+ exchange in this model of cardiac pathology.


Subject(s)
Amiloride/analogs & derivatives , Calcium/metabolism , Heart Diseases/prevention & control , Myocardium/metabolism , Amiloride/pharmacology , Animals , Heart Diseases/etiology , Heart Diseases/pathology , Male , Myocardial Contraction/drug effects , Myocardial Contraction/physiology , Myocardium/enzymology , Osmolar Concentration , Ouabain/pharmacology , Rats , Rats, Inbred Strains , Sarcolemma/metabolism , Sodium/metabolism
11.
J Pharmacol Exp Ther ; 252(1): 358-64, 1990 Jan.
Article in English | MEDLINE | ID: mdl-2105394

ABSTRACT

Equine airway smooth muscle is innervated by vagal efferents and, in addition, displays spontaneous mechanical activity. The preparation thus appears to contain at least two discrete excitable components, the cholinergic neural elements and the smooth muscle membrane. Indomethacin (INDO), a cyclooxygenase (CO) inhibitor, exerts a considerable potentiation of function in this preparation. The latter may be effected indirectly, through loss of the inhibitory effect of endogenous prostaglandin E2 (PGE2) on neural acetylcholine release and through direct effects on smooth muscle of the generally antagonistic CO and lipoxygenase (LO) metabolites. The present studies were designed to assess the relative contributions of altered arachidonic acid metabolism on those respective elements. The utility of the model, in terms of distinguishing neural and myogenic components, was assessed by examining the effects of the muscarinic antagonist atropine (ATR) and the neurotoxin tetrodotoxin (TTX) on the stimulus-response (SR) relationship. The observations that TTX and ATR produced similar rightward (but not downward) shifts of the SR curve and that D-600 inhibited the TTX-insensitive responses are consistent with a selective activation of the muscle by the nerves at lower voltages and a direct stimulation of the muscle at higher voltages. INDO potentiated both the neural and myogenic components of the SR curve, effects which were sensitive to ATR and 5,8,11,14-eicosatetraynoic acid, an inhibitor of LO, and reversed by PGE2. The finding that PGE2 at low doses (10(-8) M) inhibited responses at lower voltages and that at higher concentration (10(-7) M) it shifted the SR curve right and downward suggested that neurotransmitter release is more sensitive to PGE2 inhibition than is muscle response.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Indomethacin/pharmacology , Trachea/drug effects , 5,8,11,14-Eicosatetraynoic Acid/pharmacology , Acetylcholine/metabolism , Animals , Atropine/pharmacology , Dinoprostone/pharmacology , Horses , In Vitro Techniques , Lipoxygenase/physiology , Muscle Contraction/drug effects , Prostaglandin-Endoperoxide Synthases/physiology , Tetrodotoxin/pharmacology , Trachea/physiology
12.
Endocrinology ; 125(2): 730-5, 1989 Aug.
Article in English | MEDLINE | ID: mdl-2752974

ABSTRACT

The purpose of the present study was to examine the effects on cataractogenesis of daily sc administration of the Ca2+ antagonist drug verapamil to diabetic rats. Streptozotocin-induced diabetic rats were given verapamil half-way through the 8-week experimental period or during the full 8 weeks of diabetes. Verapamil administration had no effect on the high blood glucose values, low circulating insulin levels, or elevated triglyceride and cholesterol concentrations in the diabetic rats. Untreated diabetic rats had a 90% incidence of cataracts. Four weeks of verapamil administration reduced this incidence to 41%, and a full 8 weeks of drug treatment further lowered the incidence to 20%. Diltiazem, another Ca2+ antagonist, lowered the incidence of cataracts in the diabetic rats to a similar extent. Verapamil administration to the diabetic animals also partially protected against the presence of retinal microangiopathy in the diabetic animals. Lenticular hydration and lipid accumulation were only indirectly related to cataractogenesis in the diabetic rats and its protection by verapamil treatment. Lenticular electrolyte imbalance, particularly Ca2+, in the diabetic animals was closely correlated with cataract formation, and verapamil significantly reduced the alterations in these ion concentrations. The present results demonstrate the efficacy of verapamil as a protective agent against cataractogenesis and some retinal damage in diabetic animals. Most importantly, this occurs in the absence of any change in the glycemic status of the diabetic animals. The findings strongly support a role for lenticular Ca2+ imbalance in cataract development in diabetes and provide initial evidence to suggest its clinical use in the diabetic population at risk for blindness.


Subject(s)
Cataract/prevention & control , Diabetic Retinopathy/prevention & control , Verapamil/administration & dosage , Animals , Calcium/analysis , Cataract/drug therapy , Diabetes Mellitus, Experimental/complications , Diabetes Mellitus, Experimental/pathology , Diabetic Retinopathy/drug therapy , Diltiazem/therapeutic use , Injections , Lens, Crystalline/analysis , Lipids/analysis , Male , Microcirculation/drug effects , Rats , Rats, Inbred Strains , Retina/blood supply , Retina/drug effects , Verapamil/pharmacology , Verapamil/therapeutic use
13.
Circulation ; 77(5): 988-96, 1988 May.
Article in English | MEDLINE | ID: mdl-3359596

ABSTRACT

Despite considerable research, the mechanisms responsible for the vasospasm associated with Raynaud's syndrome are not well understood and there is no reliable diagnostic test. In the present studies, measurements of systolic pressure in locally cooled fingers were used to address these issues. We found that local cooling produced a marked decrease or loss of the apparent finger systolic pressure in patients with Raynaud's syndrome in whom a standardized vasoconstriction had been induced by body cooling. Abnormal responses were encountered in 109 of 125 patients with secondary Raynaud's syndrome, in 21 of 37 patients with primary Raynaud's disease or the syndrome of uncertain cause, and in two of 63 subjects without symptoms of Raynaud's. These data suggest a high accuracy of the test in patients with secondary Raynaud's syndrome and lower accuracy in those with disease of primary or uncertain cause. We studied responses of systolic pressures to alterations in body and local temperatures in fingers with and without low pressures secondary to proximal arterial obstruction. Our data show that although local cooling has a small independent effect that increases vascular tone: (1) sympathetic vasoconstriction induced by body cooling is necessary to produce vasospasm and often produces it without local cooling, (2) high local temperature (30 degrees C) protects from vasospasm, and (3) low finger blood pressure predisposes to it. Delayed opening of the vessels observed after sudden deflation of blood pressure cuffs suggests that abnormal responses of finger systolic pressure to cold represent combined effects of high vascular tone, delayed opening, and local blood pressure.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Cold Temperature , Fingers/physiopathology , Myocardial Contraction , Raynaud Disease/physiopathology , Systole , Adolescent , Adult , Aged , Chronic Disease , Fingers/blood supply , Humans , Middle Aged , Raynaud Disease/diagnosis , Skin Temperature , Vasoconstriction , Vasomotor System/physiopathology
15.
J Pharmacol Exp Ther ; 243(3): 907-14, 1987 Dec.
Article in English | MEDLINE | ID: mdl-3694537

ABSTRACT

Previous studies from our laboratory showed an atropine-sensitive component in the hyperresponsiveness of ragweed-sensitized canine tracheal smooth muscle (TSM) in vitro to histamine and potassium. The present studies were undertaken to elucidate the nature of the parasympathetic element in this hyperresponsiveness. TSM strips were dissected from ragweed-sensitized and littermate control dogs and their isometric force generation was measured in vitro. Mechanical responses of sensitized TSM were characterized by hyperreactivity (upward shift of the dose-response relationship) to acetylcholine (ACh), atropine-sensitive spontaneous base line activity and prolonged isometric force plateaus. Control TSM did not contract spontaneously and basal tone was maintained passively. However, eserine could produce spontaneous base-line activity and prolonged isometric force plateau in control TSM that mimicked that observed naturally in sensitized TSM. Sensitized TSM was supersensitive (leftward shift of the dose-response relationship) to ACh and electrical field stimulation, and showed a significant leftward shift of the threshold dose to carbamylcholine (carbachol). However, sensitized and control TSMs were equally reactive to carbachol at doses of 10(-8) M and greater. Also, ACh dose-response curves of sensitized and control TSMs in the presence of the cholinesterase inhibitor eserine (10(-8) M) showed no significant differences in sensitivity or reactivity. These results were consistent with a role for local parasympathetic mechanisms such as altered ACh release and/or breakdown in the hyperresponsiveness of ragweed-sensitized canine TSM.


Subject(s)
Hypersensitivity/physiopathology , Muscle, Smooth/physiology , Parasympathetic Nervous System/physiology , Trachea/physiology , Acetylcholine/metabolism , Acetylcholine/pharmacology , Animals , Carbachol/pharmacology , Dogs , Dose-Response Relationship, Drug , Electric Stimulation , Muscle Contraction , Physostigmine/pharmacology , Potassium/pharmacology , Trachea/drug effects
16.
J Pharmacol Exp Ther ; 238(2): 679-84, 1986 Aug.
Article in English | MEDLINE | ID: mdl-3735137

ABSTRACT

The effect of tone on responses of canine tracheal smooth muscle (TSM) to norepinephrine (NE) was studied to elucidate the role of sympathetic innervation and adrenoceptors in the control of the airways. Electrical field stimulation produced contraction of TSM in vitro which was augmented by eserine, depressed by phentolamine, potentiated by propranolol in the presence of K+ (14 mM) and almost eliminated by tetrodotoxin or atropine. Resting TSM did not contract in response to NE (10(-8) to 10(-4) M) in the presence or absence of propranolol (10(-5)M). The addition of NE (10(-8) to 10(-6) M) at the plateau of contraction produced by K+ (22.8 mM), histamine (10(-6) M) or acetylcholine (5 X 10(-8) M) produced a further phentolamine-sensitive contraction which was potentiated by beta adrenoceptor blockade with propranolol (10(-5) M). The addition of tyramine (10(-5) to 10(-4) M) at the plateau of contraction produced by K+ (22.8 mM) produced a further contraction which was potentiated by propranolol (10(-5) M) and reduced by phentolamine (10(-5) M). Although the response to NE in the presence of elevated tone was contractile at low concentrations of NE (10(-8) to 10(-6) M), a propranolol-sensitive relaxant response was elicited at higher NE concentrations (10(-5) and 10(-4) M). Maximum contractions to NE in the absence or presence of beta-blockade were dependent on the tone of the muscle. These findings suggest a functional adrenergic innervation of canine TSM and the presence of alpha and beta adrenoceptors which mediate contractile and relaxant responses, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Airway Resistance/drug effects , Receptors, Adrenergic/physiology , Trachea/innervation , Animals , Atropine/pharmacology , Dogs , Electric Stimulation , Female , In Vitro Techniques , Male , Norepinephrine/pharmacology , Phentolamine/pharmacology , Physostigmine/pharmacology , Potassium/pharmacology , Propranolol/pharmacology , Tetrodotoxin/pharmacology , Trachea/drug effects , Tyramine/pharmacology
17.
J Pharmacol Exp Ther ; 236(3): 803-9, 1986 Mar.
Article in English | MEDLINE | ID: mdl-3950872

ABSTRACT

The present studies were undertaken to obtain histamine (HIST) dose-response curves for tracheal smooth muscle (TSM) from an actively ragweed-sensitized canine model of asthma and to compare these results with 1) HIST dose-response data from littermate control dogs, 2) initially nonsensitized TSM passively sensitized (in vitro) to ragweed and 3) the dose-response curve to an agonist that opens primarily voltage-sensitive calcium channels, i.e., K+. Actively ragweed-sensitized TSM was significantly hyperreactive (upward shift of the dose-response curve) to HIST (1.882 kg of force produced normalized to cross-sectional area-kg/cm2 +/- 0.087 S.E. vs. littermate controls 1.151 +/- 0.253) and hypersensitive as indicated by the leftward shift in the median effective dose or ED50 (1.86 X 10(-6) +/- 0.24 vs. 5.54 X 10(-6) +/- 1.35 M). Passively sensitized TSM (using serum from ragweed-sensitized dogs) also showed a hyperreactivity to HIST when compared to control TSM incubated with control serum (1.204 +/- 0.127 vs. 0.825 +/- 0.081 kg/cm2). No significant difference was found in the ED50 values, indicating similar sensitivities. Atropine (10(-7) M) reduced the hypersensitivity of actively sensitized TSM significantly toward control values; however, the hyperreactivity persisted. Atropine did not affect responses to HIST in control TSM. Ragweed actively sensitized TSMs were also hyperreactive and hypersensitive to K+ when compared to littermate control TSM. Atropine abolished both the hyperreactivity and hypersensitivity to K+ but had no effect on the dose-response curve of control TSM to K+.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Asthma/physiopathology , Atropine/pharmacology , Muscle, Smooth/drug effects , Pollen/immunology , Trachea/drug effects , Animals , Bronchial Spasm/physiopathology , Disease Models, Animal , Dogs , Dose-Response Relationship, Drug , Histamine/pharmacology , In Vitro Techniques , Muscle, Smooth/physiopathology , Ovalbumin/immunology , Potassium/pharmacology , Receptors, Histamine/analysis , Trachea/physiopathology
18.
Biochem Biophys Res Commun ; 131(2): 750-5, 1985 Sep 16.
Article in English | MEDLINE | ID: mdl-4052074

ABSTRACT

Recently we reported that both the triphenylethylene antiestrogen tamoxifen, and the novel compound N,N-diethyl-2-[(4 phenylmethyl)-phenoxy]-ethanamine. HCl (DPPE), which is selective for the antiestrogen binding site, may be histamine antagonists and have suggested that the antiestrogen binding site may be a growth-promoting histamine receptor different from H1 and H2 (?H3). We now show that along with established H1-antagonists, tamoxifen and DPPE specifically block the histamine-induced (H1) contraction of canine tracheal smooth muscle in the order: pyrilamine = hydroxyzine greater than tamoxifen = 4-hydroxytamoxifen greater than DPPE. The H1-antagonist hydroxyzine, which competes about equally with DPPE for the antiestrogen binding site, is up to 10(3) times stronger than DPPE in blocking histamine-induced muscle contraction. This shows that H1 antagonism is distinct from binding to the antiestrogen binding site and suggests that if the latter is a histamine receptor, it is not H1; presumably tamoxifen and DPPE compete for this novel site in addition to, and with greater affinity than, H1.


Subject(s)
Histamine Antagonists/pharmacology , Tamoxifen/pharmacology , Acetylcholine/pharmacology , Animals , Dogs , Female , Histamine/pharmacology , Hydroxyzine/pharmacology , Male , Muscle Contraction/drug effects , Phosphatidylethanolamines/pharmacology , Potassium/pharmacology , Pyrilamine/pharmacology , Serotonin/pharmacology , Tamoxifen/analogs & derivatives , Trachea/physiology
19.
J Pharmacol Exp Ther ; 213(1): 150-5, 1980 Apr.
Article in English | MEDLINE | ID: mdl-7359363

ABSTRACT

Tracheal smooth muscle (TSM) from an ovalbumin sensitized canine model of allergic asthma showed hypersensitivity and hyper-reactivity to histamine (H) when compared to that from littermate controls in vitro. Mepyramine abolished H responses in TSM of both groups; it also abolished the allergic response to obalbumin of TSM from sensitized dogs. The H2 receptor agonist, 4-methyl histamine (4-MH) caused small dose-related decreases in H contractures but had no effect on carbachol- or K+-induced tension. Metiamide, an H2 antagonist, did not enhance the H contracture, suggesting the 4-MH may not be exerting a relaxant effect since H2 receptors were absent. The maximum H-induced isometric tension was potentiated when the sensitized and control muscle strips were pre-equilibrated with 4-MH. These observations are consistent with the presence in canine TSM of H1 but not relaxant H2 receptors, the release of endogenous H to the tissue during the antigen-antibody reaction, and the competition of H and 4-MH for the H1 receptors. Experiments with specific blockers also indicated that in this model the only transmitter found in the ovalbumin-induced allergic bronchospasm was histamine.


Subject(s)
Airway Resistance/drug effects , Asthma/physiopathology , Histamine/pharmacology , Anaphylaxis/physiopathology , Animals , Asthma/immunology , Dogs , In Vitro Techniques , Methylhistamines/pharmacology , Metiamide/pharmacology , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Ovalbumin/immunology , Pyrilamine/pharmacology , Receptors, Histamine H2/drug effects , Trachea/drug effects
SELECTION OF CITATIONS
SEARCH DETAIL
...