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Arch Virol ; 147(6): 1155-72, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12111426

ABSTRACT

We report on the development and characterisation of a recombinant Murray Valley encephalitis virus (MVE) envelope glycoprotein expression system that results in the secretion of subviral particles (SVPs) upon transfection of the murine fibroblast (COS-7) cell line. Initially, aspects of the physical and antigenic structure of cell-associated and secreted forms of the MVE envelope glycoproteins (prM and E) are presented. We then show that BALB/c mice inoculated with SVPs purified from pcDNA(3)-prM/E-transfected COS-7 cell supernatants are protected from lethal challenge with the virulent prototype strain MVE-1-51 and that this protection correlates with the development of a neutralising humoral immune response by the host. By contrast, prior immunisation with cell-associated, recombinant MVE envelope glycoproteins did not protect mice from challenge with MVE-1-51 and this was associated with the development of antibody that was unable to neutralise virus infectivity in vitro. These studies demonstrate that SVPs derived from the in vitro expression of recombinant MVE prM and E genes are an effective candidate vaccine for the prevention of encephalitis in the mouse model.


Subject(s)
Encephalitis Virus, Murray Valley/immunology , Encephalitis, Arbovirus/prevention & control , Recombination, Genetic , Viral Envelope Proteins/immunology , Viral Vaccines/immunology , Virion/immunology , Animals , Antibodies, Viral/blood , COS Cells , Cell Line , Chlorocebus aethiops , Encephalitis Virus, Murray Valley/genetics , Encephalitis Virus, Murray Valley/pathogenicity , Encephalitis, Arbovirus/immunology , Mice , Mice, Inbred BALB C , Neutralization Tests , Transfection , Vero Cells , Viral Envelope Proteins/genetics , Viral Envelope Proteins/metabolism , Virion/genetics , Virion/physiology , Virulence
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