ABSTRACT
GLC was used to study the composition of endocellular fatty acids of Actinomyces canosus 89 grown on a chemically defined medium and on a complex medium to which various components were added. Total lipids of the culture contain saturated and unsaturated fatty acids, from C13 to C18, with one or two double bonds. Addition of components to the medium stimulated the biosynthesis of myristic, stearic, oleic, and linoleic fatty acids. Changes in the composition of the growth medium modify the ratio between saturated and unsaturated fatty acids of total lipids, increasing the content of unsaturated fatty acids due to a higher rate of synthesis of linoleic and oleic fatty acids. An increase in the content of unsaturated fatty acids is a positive factor because these acids are involved in important physiological functions of both this organism and other living organisms.
Subject(s)
Fatty Acids/analysis , Lipids/analysis , Streptomyces/analysis , Chemical Phenomena , Chemistry , Culture MediaABSTRACT
Saturated and unsaturated fatty acids (C13-C18) have been found in the composition of lipids and triglycerides of actinomycetes by GLC. Qualitative composition of fatty acids was identical on chemically defined and organic media. Synthesis of unsaturated fatty acids by the actinomycetes was intensive (total yield over 50% and even 84.2% in Act. canosus) on the organic medium I which may be recommended for production of lipid fractions with elevated content of unsaturated fatty acids.