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Assay of serum free fatty acids by extraction-photometric procedure.

Chromý, V; Gergel, J; Voznícek, J; Krombholzová, L; Musil, J.

Clin Chim Acta ; 80(2): 327-32, 1977 Oct 15.

Article in English | MEDLINE | ID: mdl-21046

ABSTRACT

Reaction conditions for extraction-photometric determination of free fatty acids in serum were studied. The results were found to be influenced by (a) sodium chloride concentration in the copper reagent, (b) the kind of standard solution used, (c) centrifugation of the reaction mixture and (d) serum phospholipids. A micromethod based on the formation of free fatty acids-copper soaps with a stable copper reagent and sensitive indicator for copper, 2-(2-thiazolylazo)-4-methoxyphenol, is described.

Subject(s)

Fatty Acids, Nonesterified/blood , Copper , Humans , Hydrogen-Ion Concentration , Indicators and Reagents , Methods , Phosphatidylcholines , Phospholipids/blood , Photometry

[Course of immunoreactive insulin, glycemia and fatty acid levels after stimulation with glucose and aminophylline]. / Prubeh hodnot imunoreaktivního insulinu, glykemie a mastných kyselin po stimulaci glukózou a aminophyllinem

Bartos, V; Maláková, B; Speváková, A; Vísek, V; Krombholzová, L; Musil, J; Kimlová, I; Prokopec, J.

Vnitr Lek ; 21(3): 261-8, 1975 Mar.

Article in Czech | MEDLINE | ID: mdl-1119105

Subject(s)

Aminophylline/pharmacology , Antigens/metabolism , Blood Glucose/metabolism , Fatty Acids, Nonesterified/blood , Glucose/pharmacology , Insulin/metabolism , Diabetes Mellitus/metabolism , Humans , Insulin Secretion , Obesity/metabolism , Stimulation, Chemical

[Functional examination of insulin secretion in diabetic patients, in potential diabetics and in obese persons]. / Runkcní vysetrení sekrece insulinu u diabetiku, potenciálních diabetiku a osob otylých.

Bartos, V; Maláková, B; Speváková, A; Visek, V; Krombholzová, L; Musil, J; Kimlová, I; Prokopec, J.

Vnitr Lek ; 18(3): 209-16, 1972 Mar.

Article in Czech | MEDLINE | ID: mdl-5012414

Subject(s)

Diabetes Mellitus/metabolism , Insulin/metabolism , Obesity/metabolism , Glucose Tolerance Test , Humans , Insulin Secretion , Prediabetic State/metabolism , Time Factors

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