ABSTRACT
A simple, selective and quantitative estimation of tranylcypromine using liquid chromatography and electrochemical detection with apomorphine as internal standard in human plasma is described. Sample preparation is done by liquid-liquid extraction. The absolute mean recovery of apomorphine is 95%, the recovery of tranylcypromine is 81%. This method gives a simple estimation method to assay plasma concentrations of tranylcypromine in the therapeutic range (5-200 ng/ml) with good reproducibility and linearity.
Subject(s)
Tranylcypromine/blood , Aged , Chromatography, High Pressure Liquid , Female , Humans , Male , Middle AgedABSTRACT
A method of estimating urinary oxalate is described. Impurities which might otherwise interfere with the estimation are removed by means of an ion-exchange resin. The oxalate is then reduced to glycolic acid, which forms a coloured compound with chromotropic acid. It can then be estimated colorimetrically. No special equipment is required, oxalate recovery is good, and the method is reproducible and precise.
Subject(s)
Oxalates/urine , Chromatography, Ion Exchange , Colorimetry , Humans , MethodsABSTRACT
A method is described for the estimation of oxalic acid in plasma. After removing interfering substances with an ion-exchange resin, oxalic acid is reduced to glycolic acid, which forms a coloured compound with chromotropic acid. No special equipment is required. A good recovery and precision are found.
