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1.
Am J Surg Pathol ; 33(3): 339-46, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19011561

ABSTRACT

BACKGROUND: Multicentric insulinoma disease was characterized with regard to its histopathology, multiple endocrine neoplasia type 1 (MEN1) status, precursor lesions, and the risk of hyperinsulinemic hypoglycemia recurrence. METHODS: Fourteen patients with multicentric insulinoma disease were compared with 267 patients with sporadic and familial insulinomas. The tumors were classified according to the World Health Organization (WHO) criteria. The MEN1 status was defined clinically and by germline mutation analysis. Detection of the MEN1 gene locus was performed using fluorescence in situ hybridization. The surgical interventions and the duration of disease-free survival were recorded. RESULTS: Fourteen patients (5%) without evidence of MEN1 showed 53 macrotumors and 285 microtumors expressing exclusively insulin. In addition, they had small proliferative insulin-expressing monohormonal endocrine cell clusters (IMECCs). No allelic loss of the MEN1 locus was detected in 64 tumors. All but one patient had benign disease. Recurrent hypoglycemia occurred in 6/14 patients (11 recurrences; mean time to relapse 8.4 y). Thirteen patients with MEN1 (4.6%) showed 41 insulinomas and 133 tumors expressing islet hormones other than insulin. IMECCs were not detected. Allelic loss of the MEN1 locus was found in 17/19 insulinomas. Recurrent hypoglycemia occurred in 4/13 patients (4 recurrences; mean time to relapse 14.5 y). Solitary insulinomas were found in 254/281 patients (90.4%). IMECCs were absent. There was no recurrent hypoglycemia in 84 patients with benign insulinomas. CONCLUSIONS: Insulinomatosis is characterized by the synchronous and metachronous occurrence of insulinomas, multiple insulinoma precursor lesions, and rare development of metastases, but common recurrent hypoglycemia. This disease differs from solitary sporadic and MEN1-associated insulinomas.


Subject(s)
Hyperinsulinism/etiology , Hypoglycemia/etiology , Insulinoma/complications , Pancreatic Neoplasms/complications , Adult , Female , Humans , Hyperinsulinism/genetics , Hypoglycemia/genetics , In Situ Hybridization, Fluorescence , Insulinoma/genetics , Male , Middle Aged , Multiple Endocrine Neoplasia Type 1/complications , Multiple Endocrine Neoplasia Type 1/genetics , Mutation , Pancreatic Neoplasms/genetics , Retrospective Studies
2.
Gastroenterology ; 128(5): 1187-98, 2005 May.
Article in English | MEDLINE | ID: mdl-15887103

ABSTRACT

BACKGROUND & AIMS: The identification of precursor lesions has a great impact on the understanding of tumorigenesis. Precursor lesions of endocrine tumors are known to occur in the setting of the MEN1 syndrome. The aim of this study was to test the hypothesis that MEN1-associated duodenal gastrinomas originate from diffuse preneoplastic gastrin cell changes. Precursor lesions may precede the development of duodenal gastrinomas because, in contrast to sporadic gastrinomas, these tumors are usually multiple. METHODS: The distribution of endocrine cells in the nontumorous duodenal tissue was analyzed qualitatively and quantitatively for 25 patients operated on for a duodenal gastrinoma. MEN1 status was assessed clinically and by polymerase chain reaction-based mutational analysis. RESULTS: Fourteen of 25 patients with gastrinoma had proliferative, hyperplastic lesions consisting of gastrin cells in the nontumorous duodenal mucosa, similar to the gastric enterochromaffin-like cell lesions observed in chronic atrophic gastritis. All patients with Zollinger-Ellison syndrome with proven MEN1 had such proliferative gastrin cell lesions, and all patients with Zollinger-Ellison syndrome without precursor lesions were MEN1 negative. CONCLUSIONS: Duodenal gastrinomas in MEN1, but not sporadic duodenal gastrinomas, are associated with proliferative gastrin cell changes within the nontumorous mucosa. It is likely that these lesions precede the development of MEN1-associated duodenal gastrinomas.


Subject(s)
Duodenal Neoplasms/pathology , Duodenum/pathology , Gastrinoma/pathology , Multiple Endocrine Neoplasia Type 1/pathology , Precancerous Conditions/pathology , Adult , Aged , Chromogranin A , Chromogranins/genetics , DNA Mutational Analysis , DNA, Neoplasm/analysis , Duodenal Neoplasms/genetics , Female , Gastric Mucosa/pathology , Gastrin-Secreting Cells/pathology , Gastrinoma/genetics , Humans , Hyperplasia , Ki-67 Antigen/genetics , Male , Middle Aged , Multiple Endocrine Neoplasia Type 1/genetics , Polymerase Chain Reaction , Precancerous Conditions/genetics
3.
Am J Surg Pathol ; 29(4): 524-33, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15767809

ABSTRACT

Persistent hyperinsulinemic hypoglycemia (PHH) in adults that is not caused by an insulinoma is a rare and not well-characterized disease that has been named nesidioblastosis. In this study, we defined and scrutinized criteria for its histologic diagnosis, assessed its relative incidence, and discussed its pathogenesis. In pancreatic specimens from 15 adult patients with PHH in whom no insulinoma was detected and in 18 adult control patients, the endocrine tissue was screened for islet and beta-cell changes. The diagnostic reliability of the findings was checked by an interobserver analysis. The relative frequency of the disease was assessed in a series of 232 patients with PHH. Finally, genetic analysis of the menin gene was performed. Among the various indicators of islet changes, beta-cell hypertrophy characterized by enlarged and hyperchromatic beta-cell nuclei was the most significant and diagnostic finding in patients with PHH. The interobserver analysis revealed 100% specificity and 87.7% sensitivity. The hyperfunctional state of the beta-cells was not associated with changes in the subcellular distribution of insulin and proinsulin, proliferative activity, or mutations of the menin gene. Our results indicate that diffuse nesidioblastosis in adult patients with PHH resembles that seen in neonates suffering from PHH. The most important criterion for the diagnosis is the beta-cell hypertrophy. As approximately 4% of adult patients with PHH are affected by diffuse nesidioblastosis, this disease is not as rare as it has been thought to be. Pathogenetically, the defective insulin secretion could be based on a molecular defect.


Subject(s)
Hyperinsulinism/pathology , Hypoglycemia/pathology , Islets of Langerhans/pathology , Nesidioblastosis/pathology , Adolescent , Adult , Aged , Biomarkers/metabolism , Cell Proliferation , DNA Mutational Analysis , DNA Primers/chemistry , Female , Humans , Hyperinsulinism/complications , Hyperinsulinism/genetics , Hypoglycemia/complications , Hypoglycemia/genetics , Insulin/blood , Islets of Langerhans/metabolism , Male , Middle Aged , Multiple Endocrine Neoplasia Type 1/genetics , Nesidioblastosis/complications , Nesidioblastosis/genetics , Observer Variation , Pancreas/metabolism , Pancreas/pathology , Pancreas/surgery , Polymerase Chain Reaction
4.
Arterioscler Thromb Vasc Biol ; 25(1): 57-64, 2005 Jan.
Article in English | MEDLINE | ID: mdl-15539617

ABSTRACT

OBJECTIVE: Synthesis and maturation of G protein-coupled receptors are complex events that require an intricate combination of processes including protein folding, posttranslational modifications, and transport through distinct cellular compartments. Little is known concerning the regulation of G protein-coupled receptor transport from the endoplasmic reticulum to the cell surface. METHODS AND RESULTS: Here we show that the cytoplasmatic carboxy-terminal of the angiotensin AT2 receptor (AT2R) acts independently as an endoplasmic reticulum-export signal. Using a yeast two-hybrid system, we identified a Golgi membrane-associated protein termed ATBP50 (for AT2R binding protein of 50 kDa) that binds to this motif. We also cloned ATBP60 and ATBP135 encoded by the same gene as ATBP50 that mapped to chromosomes 8p21.3. Downregulation of ATBP50 using siRNA leads to retention of AT2R in inner compartments, reduced cell surface expression, and decreased antiproliferative effects of the receptor. CONCLUSIONS: Our results indicate that ATBP50 regulates the transport of the AT2R to cell membrane by binding to a specific motif within its cytoplasmic carboxy-terminal and thereby enabling the antiproliferative effects of the receptor.


Subject(s)
Golgi Apparatus/chemistry , Membrane Proteins/physiology , Membrane Transport Proteins/physiology , Protein Transport/physiology , Receptor, Angiotensin, Type 2/metabolism , Amino Acid Sequence , Animals , COS Cells/chemistry , COS Cells/metabolism , Cell Line , Cell Line, Tumor , Chlorocebus aethiops , Cytoplasm/chemistry , Membrane Transport Proteins/biosynthesis , Membrane Transport Proteins/chemistry , Membrane Transport Proteins/genetics , Mice , Molecular Sequence Data , Molecular Weight , Neuroblastoma/genetics , Neuroblastoma/pathology , PC12 Cells/chemistry , PC12 Cells/metabolism , Peptides/physiology , Protein Binding/physiology , Protein Isoforms/genetics , Protein Sorting Signals/physiology , Rats , Receptor, Angiotensin, Type 2/biosynthesis , Receptor, Angiotensin, Type 2/chemistry , Two-Hybrid System Techniques
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