Effects of taste stimulation on gastric myoelectrical activity and autonomic balance.

Background/Aim: Sham feeding, reproducing the cephalic phase of digestion, and involving combined visual, olfactory, and taste stimulation affects gastrointestinal motility and secretory functions of the digestive system, as well as the sympathetic/parasympathetic balance (SPB). In this study, we aimed to check if taste stimulation with a single flavor affects the gastric myoelectrical activity (GMA) and/or SPB. Materials and Methods: Eighteen healthy volunteers underwent, on four separate days, 30-min electrogastrographic and electrocardiographic recordings: basal, with stimulation - while keeping in the mouth an agar cube with taste-delivering substance, and postexposure. Concentrations of saccharose, NaCl, citric acid, and quinine hydrochloride within the cubes were adjusted to 100-fold the individual taste recognition thresholds. SPB was determined from the heart rate variability (HRV) analysis of the recorded electrocardiograms. Results: A moderate but statistically significant increase in tachygastria and bradygastria percentage time share was observed, regardless of the type of taste applied. Bitter taste elicited a considerable decrease in the normogastria time share (from 82.8 ± 2.5% to 73.5 ± 3.5%, P = 0.00076) and a diminution of the dominant frequency (from 3.07 ± 0.08 to 2.90 ± 0.10 cycles per minute (cpm) postexposure, P = 0.01). Sour taste brought about a drop of the dominant power (from 42.5 ± 1.1 to 40.1 ± 1.4 dB, P = 0.0015). Two tastes hindered propagation of the gastric slow waves - the average percentage of slow wave coupling decreased from 77.9 ± 3.1% to 69.5 ± 3.1% (P = 0.0078) and from 74.6 ± 2.5% to 68.2 ± 2.8% (P = 0.0054) with the bitter and the salty taste, respectively. Stimulation with sweet, salty, or sour taste evoked a significant decrease in the high frequency component of the HRV, whereas bitter taste did not affect the SPB. Conclusions: Oral stimulation with tastes subjectively perceived as unpleasant brings about disturbances of the interdigestive GMA. This, however, does not coincide with its effect upon SPB.

Scrutiny of 13C-phenylalanine breath test reproducibility.

We evaluated the reproducibility of the 13C-phenylalanine breath test (13C-PheBT). On three separate days, 21 healthy volunteers (11 F and 10 M) underwent 13C-PheBT with 100 mg l-[1-13C]phenylalanine taken orally. Short-term reproducibility was evaluated with paired examinations taken 3 days apart; paired examinations separated by 23 days (median) served for the medium-term reproducibility assessment. Expiratory air was sampled at 19 points throughout 3 h. Determined limited reproducibility of the 13C-PheBT must be taken into consideration while interpreting the results of this diagnostic tool. The results of this study imply the following conclusions: (i) From among the three parameters examined, the cumulative 13C recovery area under the curve (AUC) offers much better reproducibility than the maximum momentary 13C recovery in the expiratory air (Dmax) or the time to reach the maximum momentary 13C recovery (Tmax) (ii) Collection of the breath air samples for 2 h results in a much better reproducibility of AUC, than for 1 h only; (iii) Reproducibility of 13C-PheBT is affected neither by the duration of the time gap between repeated tests nor by gender; (iv) Comparison with data obtained formerly reveals that reproducibility of the 13C-PheBT is worse than either that of of the 13C-methacetin (13C-MBT) or the 13C-alpha-ketoisocaproic acic (13C-KICA-BT) breath tests. This finding will have to be taken into consideration while interpreting the results of this diagnostic tool.

Assessment of knowledge and selected attitudes among Silesians about effects of ultraviolet radiation on health

INTRODUCTION: Both insufficient and excessive exposure to UV radiation (UVR) lead to serious health consequences: Caucasian people living in northern Europe commonly suffer from vitamin D deficiencies, constant increasing number of melanoma incidents is alarming. AIM: Assessment of knowledge and selected attitudes among Silesians about ultraviolet radiation. MATERIAL AND METHODS: Study involved 169 adult residents of the one Silesian city. The original, anonymous questionnaire containing 25 questions about UVR was used. Respondents (85 females, 84 males) of different age and level of education were examined. RESULTS: One-third of respondents do not spend time outdoors during sunny weather. The solarium is used by 9% of the studied group. The vitamin D synthesis was a favorable UV effect known by 90% of the respondents. 51% of the group did not know about the UV importance in melanin synthesis. 55% of respondents were not aware of the significance of the SPF (Sun Protective Factor) abbreviation, and 40% of people with the vocational education were convinced that the SPF50+ provides complete sun protection. Only 44% of women and 23% of men differentiated basic skin phototypes. Respondents knew at what time of a day in spring-summer the sun should be avoided, 71% knew about the re-application of a waterproof sunscreens after bath. 64% of women and 37% of men presented the sufficient knowledge of melanoma. Only 38% of respondents paid attention to UV filters when buying sunglasses. CONCLUSIONS: Respondents were aware of the UVR value for the vitamin D synthesis, but only a small percentage declared short-term sun exposures. Women had more knowledge about negative effects of UVR. People over 50 years of age and those with vocational education had a limited knowledge about health effects of UVR.

Gastric myoelectrical activity in patients with primary biliary cirrhosis.

OBJECTIVE: To examine gastric myoelectrical activity in patients with primary biliary cirrhosis (PBC). MATERIALS AND METHODS: The study comprised 11 female PBC patients (average age 53.4 years, range 43-70) and two aged-matched control groups: 11 (53.4 years, range 37-78) healthy women, and 10 female patients with chronic hepatitis C, CHC (53.9 years, range 35-66), who were examined prior to administration of an antiviral therapy. Every subject underwent an electrogastrographic recording comprising a 30-min interdigestive and a 120-min postprandial period. RESULTS: Abnormal electrogastrograms, containing prolonged epochs of tachygastria in the postprandial phase were found in 2 out of 11 (18.2%) patients having both stage IV of the Scheuer's PBC classification, as well as in 1 patient out of 10 (10%) with CHC at stage F2 according to the METAVIR fibrosis score. CONCLUSION: Electrogastrographic abnormalities do not seem to be pathognomonic for the PBC as a disease, but rather would be considered an unspecific sequel of a morbid liver affection.

[Application of electrogastrography in pediatrics. Part I. Definition of normal ranges of parameters of an electrogastrogram in Polish children]. / Zastosowanie badania elektrogastrograficznego w pediatrii. Czesc I. Okreslenie zakresów wartosci prawidlowych parametrów elektrogastrogramu u polskich dzieci.

UNLABELLED: The study was designed to determine normative values of an electrogastrogram in healthy children. MATERIAL AND METHODS: Surface electrogastrograms were taken in the interdigestive state and after meal stimulation in 292 non-obese children in whom Helicobacter pylori infection was excluded with the use of a 13C-urea breath test. The cohort comprised the following subgroups: age 6-9 years--114 children (57 girls and 57 boys), age 10-11 years--88 children (44 girls and 44 boys), age 12-15 years--90 children (45 girls and 45 boys). Yoghurt was chosen as a test meal: 150 g (142 kcal) was given to children aged < or = 11 years, whereas children older than 11 years received 300 g (284 kcal). RESULTS: Similarly to formerly published studies the parameters describing quantitatively the gastric myoelectrical activity exhibited considerable between-subject variability. A slight but statistically significant decrease in the dominant frequency was found with increasing age of the children. The subgroup of girls aged 12-15 years had statistically lower postprandial rise in the dominant power when compared to any of the other five subgroups. CONCLUSIONS: The results obtained imply a necessity of considering peculiarities related to age and gender when an electrogastrogram is performed in children. Taking into account the disclosed substantial between-subject variability of the electrogastrographic parameters in children caution is recommended while interpreting a result of an electrogastrographic examination.

[Application of electrogastrography in pediatrics. Part II. The incidence of disturbed gastric myoelectrical activity in children suffering from recurrent abdominal pain]. / Zastosowanie badania elektrogastrograficznego w pediatrii. Czesc II. Czestosc wystepowania zaburzen mioelektrycznej czynnosci zoladka u dzieci z nawracajacymi bólami brzucha.

UNLABELLED: The study was devoted to determine the incidence of an abnormal gastric myoelectrical activity (GMA) in children suffering from recurrent abdominal pain. MATERIAL AND METHODS: Surface electrogastrograms were taken in the interdigestive state and after a meal stimulation in 187 children referred to the laboratory with the diagnosis of recurrent abdominal pain. The cohort comprised the following subgroups: age 6-11 years (33 boys and 36 girls), age 12-18 years (28 boys and 90 girls). Continuous variables characterizing quantitatively an electrogastrogram were recoded into categorical data sets, which were used further for construction of an arbitrary scale reflecting disturbances of an electrogastrographic recording: score 0--normal, score 1-2, 3-4, and 5-6 corresponding to a light, moderate, and severe disturbance of the GMA. RESULTS: Abnormal electrogastrograms were found in just over a half of the examined children (54.5%). Nevertheless, light abnormalities (score 1-2) were predominant--42.8% of the whole cohort. Moderate abnormalities were revealed in almost every eight patient (11.8%), whereas no case of severely disturbed GMA was disclosed. On the other hand as much as 45.5% children did not exhibit any abnormality of the electrogastrogram. No statistically significant differences were found when the frequency distributions of particular degrees of the GMA disturbance were compared among groups of different age and gender. CONCLUSION: A disclosure that an abnormal electrogastrogram is encountered in just over a half of the patients and the predominance of light disturbances within this group, implies that disturbed GMA is neither an inherent nor a pathognomonic pathological finding of the clinical picture of recurrent abdominal pain in children.

Comparison of multichannel electrogastrograms obtained with the use of three different electrode types.

The goal of the study was to establish if the conductive area size of recording electrodes affects the quality of a multichannel electrogastrogram. In twelve volunteers (9F, 3M, median age 24 years, range 22-28) on three separate days fasted and postprandial four-channel electrogastrograms were registered in randomized order with Red Dot class Ag/AgCl electrodes of a type: ;2222' (conductive area/total area: 2.00/10.24 cm2, ;2271' 2.54/29.64 cm2, or ;2660' 11.64/11.64 cm2 (total surface conductive!), and subsequently analyzed with a dedicated software. In the case of type 2271 and 2660 the between-electrode electrical conductivity improved at the end of the recording relative to the basal measurement, whereas type 2222 yielded a stable between-electrode electrical conductivity throughout the examination. Despite the differences in either the conductive area size or its construction, the analysis of variance on parameters describing quantitatively the multichannel electrogastrogram did not reveal a superiority of any from among the electrodes tested. Type 2271 was, however, rated the less handy among the three electrodes. Multichannel surface electrogastrography seems to be technically feasible with any type of high quality electrodes, therefore small dimensions and easy handling may favor the choice of a particular type for this examination.

Effect of single-dose administration of recombinant interferon-alpha2b on gastric myoelectrical activity in patients with chronic hepatitis C.

BACKGROUND: Upper abdominal complaints during interferon therapy may result from impaired gastric motility and/or evacuatory function. We examined the effect of acute administration of interferon on gastric myoelectrical activity (GMA) with the use of surface electrogastrography. METHODS: The study population comprised 25 patients with chronic hepatitis C. All of them were naive to interferon. On 2 days, after a 25-min basal GMA registration, in group A (5 men, 7 women, aged 44.3 +/- 2.8 years) placebo or 5 million i.u. recombinant interferon alpha-2b (IFNA) was administered s.c. and the GMA was recorded in the interdigestive state for two periods (2 h and 4 h), separated by a 15-min break. In group B (7 men, 6 women, aged 44.7 +/- 3.9 years) placebo or 5 million i.u. IFNA was injected s.c. after the ingestion of a semiliquid test meal of 364 kcal. Subsequently, the postprandial GMA was recorded for two periods (2 h and 4 h), separated by a 15-min break. RESULTS: A typical flu-like syndrome was observed in 91.7% of patients in group A, and in 92.3% of patients in group B, thus providing evidence of the pharmacodynamic efficiency of the IFNA administration. In the fasted state, IFNA brought about a negligible increase in the rhythmicity and power of the gastric slow waves. IFNA did not elicit any statistically significant effect on gastric slow-wave activity postprandially. CONCLUSIONS: Acute administration of interferon does not involve any deterioration of GMA that could be linked to the previously reported upper abdominal symptoms in patients undergoing treatment with this drug.