ABSTRACT
The series of novel monomer and homodimer styryl dyes based on (p-dimethylaminostyryl) benzothiazolium residues were synthesized and studied as possible fluorescent probes for nucleic acids detection. Spectral-luminescent and spectral-photometric properties of obtained dyes in the unbound state and in DNA presence were studied. Fluorescence emission induced by two-photon excitation of dye-DNA complexes in aqueous buffer solution was registered. Two-photon absorption cross section values of the studied dyes in DNA presence were evaluated.
Subject(s)
Benzothiazoles/chemistry , DNA/analysis , Fluorescent Dyes/chemistry , Spectrometry, Fluorescence , Styrenes/chemistry , Animals , Fluorescence , Fluorescent Dyes/chemical synthesis , PhotonsABSTRACT
Series of homodimer styryls containing on (p-dimethylaminostyryl) pyridinium residues that are connected with aliphatic linkage group was synthesized. Spectral luminescent properties of obtained dyes in free state and in nucleic acids presence were studied. It was shown that DNA binding affinity of the novel homodimers exceeds that of parent monomer (p-dimethylaminostyryl)pyridine iodide. For homodimers with the linkage 4-10 carbon atoms preference in binding to DNA than to RNA was observed. It could be concluded that parent monomer has different mechanisms of binding to nucleic acids than corresponding homodimer dye.
Subject(s)
DNA/chemistry , Fluorescent Dyes/chemistry , Animals , Chickens , Dimerization , Fluorescent Dyes/chemical synthesis , In Vitro Techniques , Luminescence , Macromolecular Substances , Molecular Structure , RNA, Fungal/chemistry , Spectrometry, Fluorescence , Spectrophotometry , Styrenes/chemical synthesis , Styrenes/chemistryABSTRACT
Fifteen polymethine cyanine dyes were studied as fluorescent stains for DNA in electrophoretic gels. Among studied cyanines, two dyes CPent V and CCyan 2-O most effectively visualized covalently closed and linear double-stranded DNA molecules in gels under standard conditions using UV-illumination, green filter and black-and-white photo film. Ethidium bromide was 1.2-1.6 times more effective as compared to cyanine dyes in staining of DNA in the concentration range of 8-18 ng, while studied cyanines were more sensitive to DNA quantity above 50 ng.
Subject(s)
Carbocyanines/chemistry , DNA/chemistry , Fluorescent Dyes/chemistry , Nucleic Acids/chemistry , DNA/analysis , Electrophoresis, Agar Gel/methods , Ethidium/chemistry , Molecular Structure , Staining and LabelingABSTRACT
Novel monomethine pyridinium cyanine dyes of similar structure and containing 'affinity-modifying' groups of different chemical nature were studied by spectral-luminescent methods as possible fluorescent probes for the nucleic acids detection. It was shown that the nature of the functional groups in the dye linker influences the fluorescent properties of the dye-nucleic acids complexes. Incorporation of a hydroxyl group into the linker structure leads to a significant increase in the fluorescence intensity of the dye--double-stranded DNA complexes relative to the parent dye Cyan 40.
