Presence and possible origin of positive Eu anomaly in shoot samples of Juncus effusus L.

BACKGROUND: The rare earth elements (REE) are non-essential elements for plants. They stimulate plant growth at low doses, but at high levels are phytotoxic. There are differences in concentrations of REE in various organs of the same plant species, but the normalized REE patterns can be very similar in samples of the same species collected in different locations. Here we compare normalized REE curves in above-ground samples of Juncus effusus L. (common rush, soft rush) collected from sites with different land-use types. METHODS: The concentrations of rare earth elements were measured in 55 shoot samples of J. effusus L. The samples were collected from 15 sampling sites located in the Holy Cross Mts., south-central Poland and analyzed with the use of inductively coupled plasma mass spectrometry (ICP-MS). The results were normalized to the North American Shale Composite and anomalies of different elements were calculated. RESULTS: Total REE concentrations varied from 0.028 mg/kg to 2.7 mg/kg. The samples were enriched in the light REE (from La to Eu) with the highest concentrations of La and Ce. The North American Shale Composite (NASC)-normalized REE curves were roughly similar in all samples except for two samples collected in the acid mine drainageaffected areas. CONCLUSION: All samples showed positive europium anomalies in NASC-normalized REE concentration patterns. The most probable explanation of this is that the uptake and translocation of Eu in J. effusus (and possibly in other wetland plants) is caused by a short-term decrease of the redox potential in a rhizosphere favoring reduction of Eu3+ to Eu2+ and thus enhancing Eu mobility in the soil-plant environment.

Seasonal changes in concentrations of trace elements and rare earth elements in shoot samples of Juncus effusus L. collected from natural habitats in the Holy Cross Mountains, south-central Poland.

Selected trace elements (Ag, As, Ba, Bi, Cd, Co, Cr, Mn, Cu, Fe, Ni, Pb, Tl, U, Zn) and rare earth elements were determined in 13 samples of Juncus effusus collected from three investigation sites in the Holy Cross Mts., south-central Poland. Sampling was carried out four times during a vegetative season of 2014. Almost all the elements examined showed different seasonal trends in their concentrations, except for Ag, Co and Ni. Maximum concentrations of Ag in samples of three investigation sites were found in May (0.068, 0.062, 0.047 mg/kg) whereas Co (0.124, 0.070, 0.079 mg/kg) and Ni (1.8, 0.998, 2.8 mg/kg) in July, respectively. Mean concentrations of Mn and Cd were higher in shoots (558 and 2.35 mg/kg) than in roots (435 and 1.7 mg/kg). Both these elements revealed much higher concentrations in J. effusus than their typical contents in plant samples. Principal component method allowed us to allocate Ni, Ba, Cd and Cu to one group with the highest positive loadings. The most probable explanation for this correlation is that bioavailability of these metals is increased by J. effusus through a release of oxygen to the rhizosphere. Light rare earth elements concentrations predominate over heavy rare earth elements in the samples examined. A fractionation of lanthanides occurs during their transport from roots to shoots, although this transport is rather limited. All shoot samples have a strong positive Eu anomaly.

Intelligent Analysis of Samples by Semiquantitative Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) Technique: A Review.

Inductively coupled plasma-mass spectrometry (ICP-MS) is a popular and routine analytical method that has been used for determination of trace elements since the 1980s. It provides fast quantitative analysis and allows the determination of more than 70 elements with good accuracy and very low detection limits, but requires an intricate calibration procedure. In analyses of samples for which very low detection limits are not required a semiquantitative ICP-MS analysis mode can be used. This approach is more time- and cost-effective, and it uses a simple calibration procedure. This article presents a critical review of the semiquantitative (SQ) mode of ICP-MS and describes current and future applications of SQ analysis.

Prospecting for hyperaccumulators of trace elements: a review.

Specific plant species that can take up and accumulate abnormally high concentrations of elements in their aboveground tissues are referred to as "hyperaccumulators". The use of this term is justified in the case of enormous element-binding capacity of plants growing in their natural habitats and showing no toxicity symptoms. An increasing interest in the study of hyperaccumulators results from their potential applications in environmental biotechnology (phytoremediation, phytomining) and their emerging role in nanotechnology. The highest number of plant species with confirmed hyperaccumulative properties has been reported for hyperaccumulators of nickel, cadmium, zinc, manganese, arsenic and selenium. More limited data exist for plants accumulating other elements, including common pollutants (chromium, lead and boron) or elements of commercial value, such as copper, gold and rare earth elements. Different approaches have been used for the study of hyperaccumulators - geobotanical, chemical, biochemical and genetic. The chemical approach is the most important in screening for new hyperaccumulators. This article presents and critically reviews current trends in new hyperaccumulator research, emphasizing analytical methodology that is applied in identification of new hyperaccumulators of trace elements and its future perspectives.