[Sensitivity of Pseudomonas sp. strain isolated from distilled water to disinfectants]. / Wrazliwosc na srodki dezynfekcyjne szczepu Pseudomonas wyizolowanego z wody destylowanej.

The aim of the study was to compare Pseudomonas sp. strain isolated from distilled water and grown on the sterile filtrate from this water with the referent strain of Pseudomonas aeruginosa NCTC 6749 grown under the standard conditions, with the respect to their sensitivity to eight common disinfectants. Partly modified EN 1040 method was applied. The reduction factor (RF) for at least five concentrations of each of the products were determined. The linear relationship between concentration of the disinfectants and RF was established in a graphic form. Sensitivity of the strains was compared at the RF = 4. The WS strain was found to be more sensitivity than the referent strain to the disinfectants. The ratio concentrations of the solutions that were effective during 15 minutes against both the strains were; 1.3 for ethyl alcohol; 2.1 for glutaraldehyde; 1.7 for formalin; 1.85 for phenol. In the case of chloramine-T, the sodium salt of dichloroisocyanuric acid, and peracetic acid the ratios of concentrations were 156.0; 96.5 and 21.5 respectively. They indicate much higher sensitivity of the strain isolated from distilled water to these chemicals.

[The action of ultraviolet radiation on microorganisms found in air]. / Dzialanie promieniowania UV na drobnoustroje znajdujace sie w powietrzu.

The effect of a device with UV lamp on the microorganisms in the air was investigated. The air was continuously pumped through the device for 24 h. After that time the number of microorganisms was reduced by 0.3 log.

[The effect of growth media on recovery of test microorganisms after exposure to saturated steam under pressure]. / Wplyw podlozy wzrostowych na odzysk drobnoustrojów testowych poddanych dzialaniu pary wodnej w nadcisnieniu.

The aim of this study was to find out which growth media give the best condition for the development of test bacteria after exposure to saturated steam under pressure. The test organisms were strains of Bacillus subtilis NCTC 3610 and Bacillus stearothermophilus NCTC 8923. The test prepared from spore suspension were exposed to saturated steam under pressure 0.2 atn-B.subtilis, and 0.7 atn-B. stearothermophilus with various length of exposure /sublethal conditions/. After the exposure the tests were placed in growth media. The obtained results show that the compositions of the medium in which spore-forming bacteria are grown after the exposure under sublethal conditions to saturated steam under pressure affects the recovery of the test organism. The media with glucose, tryptose and L-alanine provided the best conditions for growth.

[Factors modulating the sensitivity of bacterial spores to steam under pressure]. / Czynniki modelujace wrazliwosc spor bakterii na dzialanie pary wodnej w nadcisnieniu.

The sensitivity of spores B. subtilis and B. stearothermophilus to steam under pressure depended on the growth medium and duration of cultivation. B. subtilis and B. stearothermophilus produced the largest number of spores on medium with Mn and yeast extract. However the spores grown on the medium were not the most resistant. The resistant spores was growing up with the age of cultures. The highest level of resistance was obtained in the case of the medium with Ca, after 7-10 days of cultivation. The sporicidal effect of steam under pressure depended on the number of spores on the test.

[Microbiological contamination of disinfectants used in hospitals for the disinfection of medical instruments. Part II: The estimation of sensitivity of isolated strains of bacteria in disinfectants]. / Zanieczyszczenie mikrobiologiczne plynów stosowanych w szpitalach do dezynfekcji narzedzi lekarskich. Czesc II. Okreslenie wrazliwosci wyizolowanych szczepów bakterii na srodki dezynfekcyjne.

The sensitivity, on basic disinfectants, of 30 strains of bacteria isolated from disinfecting fluid used in hospitals were estimated. The sensitivity of isolated strains were compared with that of laboratory test strains.

[Bacterial age and their susceptibility to disinfectants]. / Wrazliwosc wybranych drobnoustrojów na srodki dezynfekcyjne w zaleznosci od wieku hodowli.

The comparative tests were carried out on 4 standard test microorganisms grown 24 h, 48 h, 72 h, with regard to their susceptibility to 6 disinfectants. The activity of disinfectants was examined by Berry and Bean method--mean single survival time (MSST). The results indicate that 24 h grown bacteria have the lowest susceptibility to phenol, Septyl, formalin, glutaraldehyde, chloramine and Sterinol. The MSST of these short time grown bacteria was 2-3 times longer than that of 72 h grown.

[The effect of temperature on bactericidal activity of certain disinfectants]. / Okreslenie wplywu temperatury na aktywnosc bakteriobójcza niektórych srodków dezynfekcyjnych.

Increase of the temperature by 10 degrees C (on the temperature range 22-32 degrees C) grows up the bactericidal activity of the disinfectants like Laurosept, Chloramine, Sterinol and Jodoseptan against E. coli and S. aureus. It has not observed the influence on the bactericidal activity of Septyl against S. aureus. Surface-active agents are more effective at higher temperature against S. aureus, phenolic disinfectants and Chloramine against E. coli.

[Microbiological contamination of disinfectants used in the hospitals for the disinfection of medical instruments. Part I]. / Zanieczyszczenie mikrobiologiczne plynów stosowanych w szpitalach do dezynfekcji narzedzi lekarskich. Czesc I.

Among the 10 bactericidal agents which were used in the hospitals to disinfection of medical instruments, Aldesan was the preparation exerting the strongest bactericidal effect. The highest bacterial counts were observed in the used solutions of Sterinol and preparations containing chlorhexidinum gluconicum. The bacterial contamination of ethyl alcohol 70% v/v shows that this solution should not be used to disinfection of "clean" medical instruments. The considerable proportion of samples contained Gram-positive cocci. It is reasonable, therefore, to compare the sensitivity of bacteria strains isolated from hospitals to disinfectants with that of the laboratory test strains.

[Evaluation of disinfection procedures conducted in hospitals in Poland from the aspect of hospital infection control]. / Ocena zabiegów dezynfekcyjnych wykonywanych w placówkach sluzby zdrowia w Polsce w aspekcie zwalczania zakazen szpitalnych.

The physical and chemical procedures of disinfection presently employed in hospitals are described. It is emphasized that not all technical and organizational appliances, currently in use, satisfy the requirements. Propositions aimed at improving this situation are presented.

[Sensitivity of surface microorganisms to disinfectants]. / Wrazliwosc na srodki dezynfekcyjne drobnoustrojów znajdujacych sie na powierzchniach.

The influence of humidity and temperature on survival of S. aureus and P. aeruginosa on the surfaces of titles, glass and blanket carriers has been estimated. The number of CFU was examined after exposure time 6 and 24 hours in temperatures of 21 degrees C, 37 degrees C and RH 35%, 95%. It was observed: 1. The important reduction of numbers of both microorganisms at temperature 37 degrees C and RH 95%, 2. The relatively high number of survival cells of P. aeruginosa on the surface of blankets at temp. 21 degrees C and RH 95%. The microorganisms on the carriers were previously kept for 24 h at temp. 21 degrees C, RH 35% and 95% and then exposed to solutions of chloramine, formalin, lysol and Sterinol (QAC). It was observed that there was a great dependence of the disinfecting effect on the degree of dessication of the surfaces. In all cases the resistance of contaminated carriers stored 24 h was higher at 95% RH than at 35% RH.

[Survival of microorganisms on surfaces in relation to moisture and temperature and the effect of desiccation of microorganisms on resistance to disinfectants]. / Uberlebensfähigkeit von Mikroorganismen auf Flächen in Abhängigkeit von Feuchtigkeit und Temperatur und Einfluss der Austrocknung der Mikroorganismen auf die Desinfektionsmittelresistenz.

In laboratory tests with carriers (glass, slab, blanket) at a high rel. humidity (95%) the survival was distinctly reduced by S. aureus at 37 degrees C and by Ps. aeruginosa at 21 degrees C and 37 degrees C. Against this after 24 h storing of contaminated carriers (glass) the resistance to disinfectants was higher at 95% rel. humidity as at 35% rel. humidity.