ABSTRACT
PURPOSE OF INVESTIGATION: The aim of the study was to determine the activity of cathepsin D (CTSD) and alpha-1-antitrypsin (AAT) in the blood serum of women with cervical carcinoma treated with different modes of therapy. METHODS: The study was conducted on 68 women suffering from carcinoma of the uterine cervix, that were irradiated intracavitarily by a Selectron LDR brachytherapy unit. Additionally, all patients were treated with different therapy methods according to clinical stage. RESULTS: In women with cervical cancer, CTSD activity was higher while AAT activity was lower both before and after brachytherapy sessions as compared to controls. Six months after the end of therapy, the activity of CTSD and AAT reverted back to the values characteristic for healthy women. CONCLUSION: The estimation of cathepsin D and alpha-1-antitrypsin activity during the course of cervical cancer management may be useful in early detection of potential recurrence and/or widespread metastasis formation.
Subject(s)
Biomarkers, Tumor/blood , Cathepsin D/blood , Neoplasm Recurrence, Local/blood , Uterine Cervical Neoplasms/therapy , alpha 1-Antitrypsin/blood , Antineoplastic Agents/therapeutic use , Brachytherapy , Case-Control Studies , Female , Humans , Middle Aged , Radiotherapy, Adjuvant , Uterine Cervical Neoplasms/bloodABSTRACT
PURPOSE OF INVESTIGATION: The aim of the study was to determine the effect of different types of management on the activity of catalase (CAT) and glutathione peroxidase (GPx) in women with cervical carcinoma. METHODS: The patients were divided into three groups according to the mode of treatment. Patients from the first group were treated brachytherapy prior to surgery. The second group received teletherapy before brachytherapy and additionally chemotherapy. The third group was treated with teletherapy after brachytherapy sessions. RESULTS: CAT activity was higher while GPx activity was lower before and during therapy in all groups as compared to controls. Six months after the end of therapy, the activity of studied enzymes reached the values characteristic of healthy women. No significant differences in enzyme activity among the three groups were revealed. CONCLUSION: Normalization of CAT and GPx activity may prove the efficacy of applied therapy in cervical cancer patients, however enzyme activity recovery was not dependent on treatment mode.
Subject(s)
Catalase/blood , Erythrocytes/enzymology , Glutathione Peroxidase/blood , Uterine Cervical Neoplasms/therapy , Female , Humans , Uterine Cervical Neoplasms/blood , Uterine Cervical Neoplasms/enzymology , Uterine Cervical Neoplasms/radiotherapy , Uterine Cervical Neoplasms/surgeryABSTRACT
UNLABELLED: THE AIM of this study was to determine the activity of cathepsin D and alpha(1)-antitrypsin in the blood serum of patients with mammary carcinoma. PATIENTS AND METHODS: The study was conducted on 52 women operated for a unilateral breast tumor, divided into two groups, according to the number of metastases and tumor size. Cathepsin D activity was determined using the method of Anson, while alpha(1)-antitrypsin activity was determined according to the Eriksson method. RESULTS: Both groups of patients with mammary carcinoma were found to have higher activity of cathepsin D before the treatment compared to healthy females. After the surgery the enzyme activity increased significantly, whereas 6 months after the surgery it generally decreased. The activity of alpha(1)-antitrypsin was significantly lower in patients before the treatment than in the controls, while after 6 months an increase in alpha(1)-antitrypsin activity was observed. The correlation between activity of cathepsin D and alpha(1)-antitrypsin was revealed. High enzyme activity and low alpha(1)-antitrypsin activity may result from the stage of neoplastic transformation. CONCLUSION: The determination of cathepsin D activity together with alpha(1)-antitrypsin activity may serve as useful biochemical marker in monitoring of malignant changes in breast tumor.
Subject(s)
Breast Neoplasms/enzymology , Cathepsin D/metabolism , alpha 1-Antitrypsin/metabolism , Adult , Aged , Aged, 80 and over , Breast Neoplasms/pathology , Cathepsin D/blood , Cell Transformation, Neoplastic , Female , Humans , Middle Aged , Neoplasm MetastasisABSTRACT
Prevalence of mitochondrial diseases equals 1:10,000 of life-born infants. Mutations of mitochondrial DNA are their most frequent cause. The study presents short description of some of these diseases. The diseases often result in encephalomyopathy, cardiomyopathy, vision disorders, dysacusis and metabolic disorders. Despite numerous studies problems associated with mitochondrial diseases have not been completely solved yet.
