ABSTRACT
All ten compounds were isolated from the methanolic extract of the whole plants of Diodia teres through repeated silica gel and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as three iridoid glycosides, asperuloside, geniposidic acid and asperulosidic acid, a coumarin glycoside, scopolin, and six flavonoids, rutin, kaempferol-3-O-rutinoside, quercitrin, astragalin, isoquercitrin and quercetin by spectroscopic analysis.
Subject(s)
Plant Structures/chemistry , Plants, Medicinal/chemistry , Rubiaceae/chemistry , Chemistry, Pharmaceutical/methods , Chromatography, Liquid/methods , Coumarins/chemistry , Coumarins/isolation & purification , Flavonoids/chemistry , Flavonoids/isolation & purification , Glucosides/chemistry , Glucosides/isolation & purification , Glycosides/chemistry , Glycosides/isolation & purification , Iridoid Glucosides , Iridoids/chemistry , Iridoids/isolation & purification , Kaempferols/chemistry , Kaempferols/isolation & purification , Methanol/chemistry , Plant Extracts/chemistryABSTRACT
The antioxidant activity of Juniperus chinensis (Cupressaceae) was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl). The methanolic extract of J. chinensis heartwood showed the strong antioxidant activity. The antioxidant activity of n-BuOH soluble fraction was stronger than that of the others, and the fraction was subjected to purification by repeated silica gel and Sephadex LH-20 column chromatography. Quercetin, naringenin, taxifolin, aromadendrin and isoquercitrin were isolated from the n-BuOH fraction. Their structures were elucidated by physico-chemical and spectroscopic studies.
