ABSTRACT
Stroke is the most common cause of adult disability in developed countries, largely because spontaneous recovery is often incomplete, and no pharmacological means to hasten the recovery exist. It was recently shown that mesencephalic astrocyte-derived neurotrophic factor (MANF) induces alternative or M2 activation of immune cells after retinal damage in both fruit fly and mouse and mediates retinal repair. Therefore, we set out to study whether poststroke MANF administration would enhance brain tissue repair and affect behavioral recovery of rats after cerebral ischemic injury. We used the distal middle cerebral artery occlusion (dMCAo) model of ischemia-reperfusion injury and administered MANF either as a recombinant protein or via adeno-associated viral (AAV) vector. We discovered that, when MANF was administered to the peri-infarct region 2 or 3 days after stroke, it promoted functional recovery of the animals without affecting the lesion volume. Further, AAV7-MANF treatment transiently increased the number of phagocytic macrophages in the subcortical peri-infarct regions. In addition, the analysis of knockout mice revealed the neuroprotective effects of endogenous MANF against ischemic injury, although endogenous MANF had no effect on immune cell-related gene expression. The beneficial effect of MANF treatment on the reversal of stroke-induced behavioral deficits implies that MANF-based therapies could be used for the repair of brain tissue after stroke.
Subject(s)
Astrocytes/metabolism , Nerve Growth Factors/genetics , Stroke Rehabilitation , Stroke/metabolism , Animals , Behavior, Animal , Brain Ischemia/complications , Dependovirus/genetics , Disease Models, Animal , Gene Expression , Genetic Vectors/genetics , Humans , Magnetic Resonance Imaging , Male , Nerve Growth Factors/metabolism , Rats , Stroke/diagnosis , Stroke/etiology , Transduction, Genetic , TransgenesABSTRACT
Skull bone metastasis from the gastric cancer is a rare. We reported a 52-year-old male proven to have stomach cancer and who underwent subtotal gastrectomy 5 months previously. Within the past 2 weeks, he experienced headache and dizziness followed by weakness of the left lower limb. A diagnosis of right chronic subdural hematoma (SDH) was made, and a burr hole was performed for drainage of the hematoma. However, 5 days later, the patient had sudden loss of consciousness without external evidence of trauma to the head. An emergency computed tomography (CT) scan revealed a high-density lesion consistent with an acute SDH on the right side. Histologically, metastasis of gastric adenocarcinoma was detected in both the dura mater and skull. It is suggested that the SDH was caused by the rupture of vessels in the metastasis or rupture of the inner dural vessels, possibly resulting from congestion of the outer vessels.
