Effects of soy isoflavone consumption on bone structure and milk mineral concentration in a rat model of lactation-associated bone loss.

BACKGROUND: Like menopause, during complete lactation, circulating estrogen concentrations are markedly reduced, resulting in amplified bone resorption. AIM OF STUDY: To investigate the effects of soy isoflavones, common dietary components used to mitigate the bone loss of menopause, on the bone loss associated with lactation. METHODS: Lactating rats were randomized to one of four diets supplemented with different levels of soy isoflavones (0, 2, 4, 8 mg aglycone isoflavone/g protein). Milk was collected from all dams between days 12 and 15 of lactation and was analyzed for calcium, phosphorus and genistein concentrations. Serum and bones from half of the animals from each diet group were taken at weaning and from the remaining half at 4 weeks post-weaning. Bones underwent histomorphometric analysis and serum was used for genistein determinations. RESULTS: Serum genistein and milk concentrations reflected dietary isoflavone dose. Isoflavone intake had no effect on any of the bone changes associated with lactation or recovery. Milk calcium and mineral concentrations were unaffected by dietary isoflavones. CONCLUSIONS: Consumption of soy isoflavones, in levels that can be readily attained through soy foods, have neither protective effects on bone nor deleterious effects on milk quality or quantity during lactation.

Lack of skeletal effect of soy isoflavones in intact growing, female rats may be explained by constant serum estrogenic activity despite reduced serum estradiol.

BACKGROUND/AIMS: Scarce data exist on the effects of soy isoflavones (IF) on bone during peripuberty, a known 'window of opportunity' for bone consolidation. Our aim was to determine the skeletal, reproductive, and serum estradiol (E(2))/estrogenic activity response of consuming naturally-occurring soy protein-associated IF during peripuberty. METHODS: Weanling (approximately 3 weeks old), female rats were placed on one of four nutritionally-complete dietary regimens in which protein (200 g/kg diet) was provided as casein or soy protein isolates containing either 0.11 (Low IF), 2.16 (Med IF), or 3.95 (High IF) mg total aglycone isoflavones/g protein for 8 weeks, during which body weights and estrus cycling were recorded. RESULTS: Bone growth and density were unaffected by soy intake while the reproductive tissues showed a slight response (greater uterine weights of the Med and High IF groups). Despite suppression of E(2) concentrations in the High IF group, total circulating estrogenic activity was unaltered. Moreover, in the High IF group, E(2) was significantly depressed compared with bioassayable estrogenic activity, suggesting negative feedback inhibition of E(2) by the elevated circulating levels of IF. CONCLUSIONS: This suppression in E(2) with maintenance of total serum estrogenicity in the High IF group may explain the lack of effect observed in the skeletal tissues.

Psychosocial correlates of disordered eating in female collegiate athletes: validation of the ATHLETE questionnaire.

Female athletes may be at greater risk for disordered eating than their nonathletic peers, but the psychological antecedents of this dysfunctional behavior in athletes have yet to be elucidated. The objective of this study was to develop an athletics-oriented measure of psychological predictors of disordered eating and to test its initial reliability and validity. Female athletes from 3 National Collegiate Athletics Association (NCAA) Division I universities completed the ATHLETE, a written questionnaire designed to assess psychosocial factors associated with disordered eating in athletes. Five distinct and internally consistent factors (Drive for Thinness and Performance, Social Pressure on Eating, Performance Perfectionism, Social Pressure on Body Shape, and Team Trust) were positively associated with and predictive of disordered eating behaviors in female athletes. The ATHLETE is a reliable and valid measure of psychological predictors of disordered eating in athletics and will be useful in studying the etiology of disordered eating in female athletes.