Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Scand J Immunol ; 60(6): 574-83, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15584968

ABSTRACT

The pathogenesis of the amyloid deposition diseases is poorly understood. The CE/J mouse, which is naturally protected from amyloid A (AA) protein amyloidosis, has provided a tool to study mechanisms that may be implicated in amyloid deposition diseases by means of comparison of findings with those in an AA-susceptible mouse strain. We have compared proteoglycan/glycosaminoglycan accumulation in vivo in amyloid-protected CE/J mouse strain and in AA-susceptible CBA/J mouse strain in homeostasis and when injected with amyloid-inducing agents. Results indicate that there is an overall increase in [(35)S]proteoglycan/glycosaminoglycan accumulation in the spleens of both strains of mice, but with a specific increase in heparan sulfate in only CBA/J mouse spleens that are rich in amyloid. Further, we report the absence of heparan sulfate in the splenic perifollicular areas of amyloid-free CE/J mouse, whereas in the amyloid-laden CBA/J mouse there is co-localization of heparan sulfate with the AA deposits. We have also examined the glycosaminoglycan disaccharide products in both these strains of mice for their sulfation positions and found no differences in the disaccharide composition of chondroitin/dermatan sulfate and heparan sulfate isolated from the control CBA/J and control CE/J mice. There were no differences in chondroitin/dermatan sulfate in both strains after experimental induction. However, analysis of the heparan sulfate disaccharides by means of capillary high-performance liquid chromatography linked to microelectrospray ionization time-of-flight mass spectrometry indicated that the disaccharide composition of the splenic heparan sulfate obtained from the treated CBA/J mice that had developed amyloid was markedly different from that obtained from the control CBA/J mice and the treated amyloid-resistant CE/J mice. These findings suggest that unique heparan sulfates play a fundamental role in the pathogenesis of amyloid.


Subject(s)
Amyloidosis/metabolism , Glycosaminoglycans/metabolism , Serum Amyloid A Protein/metabolism , Spleen/metabolism , Amyloidosis/genetics , Animals , Genetic Predisposition to Disease , Glycoproteins/pharmacology , Glycosaminoglycans/chemistry , Immunohistochemistry , Mass Spectrometry , Mice , Mice, Inbred CBA , Proteoglycans/metabolism , Silver Nitrate/pharmacology
2.
J Biol Chem ; 278(19): 17121-9, 2003 May 09.
Article in English | MEDLINE | ID: mdl-12604602

ABSTRACT

Fibroblast growth factor (FGF) signaling begins with the formation of a ternary complex of FGF, FGF receptor (FGFR), and heparan sulfate (HS). Multiple models have been proposed for the ternary complex. However, major discrepancies exist among those models, and none of these models have evaluated the functional importance of the interacting regions on the HS chains. To resolve the discrepancies, we measured the size and molar ratio of HS in the complex and showed that both FGF1 and FGFR1 simultaneously interact with HS; therefore, a model of 2:2:2 FGF1.HS.FGFR1 was shown to fit the data. Using genetic and biochemical methods, we generated HSs that were defective in FGF1 and/or FGFR1 binding but could form the signaling ternary complex. Both genetically and chemically modified HSs were subsequently assessed in a BaF3 cell mitogenic activity assay. The ability of HS to support the ternary complex formation was found to be required for FGF1-stimulated cell proliferation. Our data also proved that specific critical groups and sites on HS support complex formation. Furthermore, the molar ratio of HS, FGF1, and FGFR1 in the ternary complex was found to be independent of the size of HS, which indicates that the selected model can take place on the cell surface proteoglycans. Finally, a mechanism for the FGF.FGFR signaling complex formation on cell membrane was proposed, where FGF and FGFR have their own binding sites on HS and a distinct ternary complex formation site is directly responsible for mitogenic activity.


Subject(s)
Fibroblast Growth Factor 1/metabolism , Heparitin Sulfate/metabolism , Receptor Protein-Tyrosine Kinases/metabolism , Receptors, Fibroblast Growth Factor/metabolism , Signal Transduction , Cell Line , Humans , Protein Binding , Protein Conformation , Receptor, Fibroblast Growth Factor, Type 1
3.
FASEB J ; 16(6): 539-45, 2002 Apr.
Article in English | MEDLINE | ID: mdl-11919156

ABSTRACT

Heparan sulfate (HS) is a sulfated polysaccharide present on cell surfaces and in the extracellular matrix. Accumulating evidence shows that HS plays key roles in many biological systems by interacting with various proteins in a structural-specific manner. Due to technical difficulties, however, the understanding of critical functional groups on HS for protein interaction is vague. We report a rapid, convenient, sensitive, and inexpensive strategy using in vitro modification with pure enzymes and gel mobility shift assay to study the subject. We demonstrated the requirements of 3-O, 6-O sulfates and the minimal length of oligosaccharide for antithrombin III (AT-III) binding. We regenerated the binding sites for AT-III on completely desulfated N-resulfated heparin and revealed the critical modification enzymes. This new strategy could be used to identify critical functional groups on HS and to generate HS library and biologically active HS, providing information applicable to the design of HS drugs, such as anticoagulant reagents and viral infection blockers. The binding assay with fibroblast growth factors and receptors confirmed the general usefulness of this approach.


Subject(s)
Electrophoretic Mobility Shift Assay/methods , Heparitin Sulfate/chemistry , Heparitin Sulfate/physiology , Animals , Antithrombin III/metabolism , Binding Sites , COS Cells , Carbohydrate Sequence , Fibroblast Growth Factors/metabolism , Molecular Sequence Data , Oligosaccharides/metabolism , Receptors, Fibroblast Growth Factor/metabolism , Sulfotransferases/metabolism
4.
Org Lett ; 2(21): 3361-3, 2000 Oct 19.
Article in English | MEDLINE | ID: mdl-11029211

ABSTRACT

The reaction of tert-butyl (4,5,7, 8-tetra-O-acetyl-3-deoxy-alpha-D-manno-2-octulopyranosyl chloride)onate donor 7 with the 6-formylgalactopyranoside acceptor 4 in the presence of SmI(2) provided only the KDO alpha-C-disaccharide 8. The bulky tert-butyl ester in the donor was used to reverse the stereochemical outcome of C-glycosylation, stereoselectively forming the alpha-"C"-disaccharide of KDO.


Subject(s)
Disaccharides/chemistry , Glycosides/chemical synthesis , Carbohydrate Sequence , Glycosides/chemistry , Nuclear Magnetic Resonance, Biomolecular
5.
Glycobiology ; 10(10): 1033-9, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11030749

ABSTRACT

Eight oligosaccharides were prepared from dermatan sulfate (DS) and their structures were elucidated. Porcine intestinal mucosal DS was subjected to controlled depolymerization using chondroitin ABC lyase (chondroitinase ABC). The oligosaccharide mixture formed was fractionated by low-pressure gel permeation chromatography (GPC). Size uniform mixtures of disaccharides, tetrasaccharides, hexasaccharides, octasaccharides, decasaccharides, and dodecasaccharides were obtained. Each size-fractionated mixture was then purified on the basis of charge by repetitive semi-preparative strong-anion-exchange (SAX) high-performance liquid chromatography (HPLC). This approach has led to the isolation of six homogeneous oligosaccharides. The size of the oligosaccharides were determined using GPC-HPLC. Treatment of tetrasaccharide and hexasaccharide fragments with Hg(OAc)2 afforded trisaccharide and pentasaccharide products, respectively. The purity of the oligosaccharides obtained was confirmed by analytical SAX-HPLC, and capillary electrophoresis (CE). The molecular mass and degree of sulfation of the eight purified oligosaccharides were elucidated using electrospray ionization (ESI) mass spectrometry and their structures were established with high field nuclear magnetic resonance (NMR) spectroscopy. These DS-oligosaccharides are currently being used to study for interaction of the DS with biologically important proteins.


Subject(s)
Dermatan Sulfate/chemistry , Oligosaccharides/chemistry , Animals , Chondroitin ABC Lyase , Chromatography, Gel , Intestinal Mucosa/chemistry , Mercury , Nuclear Magnetic Resonance, Biomolecular , Spectrometry, Mass, Electrospray Ionization , Swine
6.
Glycoconj J ; 17(12): 829-34, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11511807

ABSTRACT

Dermatan sulfate was partially depolymerized with chondroitin ABC lyase to obtain an oligosaccharide mixture from which an unsaturated disulfated tetrasaccharide was purified and characterized using nuclear magnetic resonance spectroscopy and electrospray ionization mass spectrometry. Chemical removal of the unsaturated uronate residue with mercuric acetate, followed by de-4-O-sulfation with arylsulfatase B (N-acetylgalactosamine 4-sulfatase) and N- acetylhexosaminidase catalyzed removal of the 2-acetamido-2-deoxy-D-galactospyranosyl residue at the non-reducing end afforded a monosulfated disaccharide of the structure alpha-L-idopyranosyluronic acid (1-->3)-alpha,beta-D-2-acetamido-2-deoxy-4-O-sulfo galactopyranose. This monosulfated disaccharide serves as a substrate for mammalian alpha-L-iduronidase as demonstrated using fluorophore assisted carbohydrate electrophoresis.


Subject(s)
Dermatan Sulfate/biosynthesis , Dermatan Sulfate/chemistry , Iduronidase/metabolism , N-Acetylgalactosamine-4-Sulfatase/metabolism , Animals , Chondroitin ABC Lyase/metabolism , Dermatan Sulfate/metabolism , Electrophoresis, Polyacrylamide Gel , Fluorescence , Intestinal Mucosa , Magnetic Resonance Spectroscopy , Molecular Structure , Oligosaccharides/biosynthesis , Oligosaccharides/chemistry , Spectrometry, Mass, Electrospray Ionization , Swine
7.
Glycoconj J ; 16(6): 271-81, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10579696

ABSTRACT

Glycoproteins commercially available in multi-gram quantities, were used to prepare milligram amounts of neoglycoproteins. The glycoproteins bromelain and bovine gamma-globulin were proteolyzed to obtain glycopeptides or converted to a mixture of glycans through hydrazinolysis. The glycan mixture was structurally simplified by carbohydrate remodeling using exoglycosidases. Glycopeptides were biotinylated using N-hydroxysuccinimide activated-long chain biotin while glycoprotein-derived glycans were first reductively aminated with ammonium bicarbonate and then biotinylated. The resulting biotinylated carbohydrates were structurally characterized and then bound to streptavidin to afford neoglycoproteins. The peptidoglycan component of raw, unbleached heparin (an intermediate in the manufacture of heparin) was similarly biotinylated and bound to streptavidin to obtain milligram amounts of a heparin neoproteoglycan. The neoglycoconjugates prepared contain well defined glycan chains at specific locations on the streptavidin core and should be useful for the study of protein-carbohydrate interactions and affinity separations.


Subject(s)
Bromelains/chemistry , Proteoglycans/chemistry , gamma-Globulins/chemistry , Animals , Biotinylation , Carbohydrate Sequence , Cattle , Chromatography, Agarose , Heparin Lyase/chemistry , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Proteoglycans/isolation & purification , Streptavidin/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...