Subject(s)
Syringomyelia/genetics , Child , Child, Preschool , Female , Genes, Recessive , Humans , MaleABSTRACT
Apolipoprotein B (apoB), an apolipoprotein associated with very low density lipoproteins and the atherogenic low density lipoproteins (LDL), directs the metabolism of lipoprotein particles in plasma by interacting with the LDL receptor. Utilizing human intestinal biopsy organ cultures, we have studied the synthesis of intestinal apoB in man. Intestinal organ cultures from normal adults (n = 6) were incubated in the presence of protease inhibitors in media supplemented with [35S]methionine. Media from these cultures were evaluated by sequential NaDodSO4 polyacrylamide gel electrophoresis, radioautography, and Western blot analyses, and intestinal biopsies were studied using immunohistochemistry. The relative abundance of apoB-100 and apoB-48 mRNA was assessed using reverse transcriptase-polymerase chain reaction followed by primer extension. Although apoB-48 was the principal isoprotein that was newly synthesized by intestinal organ cultures, apoB-100 was also synthesized and secreted by human intestinal organ cultures with 16 +/- 3% of the intestinal apoB mRNA coding for apoB-100. These results establish that apoB-100 is produced by the human intestine. The synthesis of the atherogenic apoB-100 by the intestine has pathophysiologic implications for the development of diet-induced atherosclerosis.
Subject(s)
Apolipoproteins B/biosynthesis , Intestinal Mucosa/metabolism , Adult , Apolipoprotein B-100 , Apolipoprotein B-48 , Apolipoproteins B/genetics , Base Sequence , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Humans , Immunohistochemistry , Molecular Sequence Data , Organ Culture Techniques , Protease Inhibitors/metabolism , RNA, Messenger/analysis , RNA, Messenger/geneticsSubject(s)
Duodenal Ulcer/radiotherapy , Laser Therapy , Child , Chronic Disease , Duodenal Ulcer/economics , Female , Humans , Male , Time Factors , USSRSubject(s)
Echinococcosis, Hepatic/diagnosis , Liver/parasitology , Adolescent , Child , Echinococcosis, Hepatic/surgery , Humans , Liver/pathology , Liver/surgery , Male , UltrasonographySubject(s)
Pediatrics/methods , Physical Therapy Modalities/methods , Child , Humans , Psychotherapy/methods , USSRABSTRACT
The interest in the studies of the intestines using the method of tissue organ culture has considerably grown in recent years. It can be explained by the great possibilities of obtaining unique data about the state of intestines in normal and pathological condition, e.g. malabsorption syndrome. The paper deals with the method modified by the authors to obtain long-living (24 hours) intestinal tissue organ culture. The investigations used bioptic sections which were obtained by jejunoscopy with spot biopsy of children suffering from intestinal malabsorption. The viability of the explants was proved by histological and histochemical tests. The promise held by the methods is emphasized.
Subject(s)
Jejunum/pathology , Tissue Survival , Biopsy , Child , Culture Media , Culture Techniques/methods , Endoscopy , Humans , Malabsorption Syndromes/pathology , Time FactorsABSTRACT
Altogether 31 children with chronic gastritis were examined. Three diagnostic tests were employed for detecting Campylobacter pylori. The clinical signs of the disease and the endoscopic features of the gastric mucosa are described.
Subject(s)
Campylobacter Infections/diagnosis , Gastritis/diagnosis , Adolescent , Campylobacter/isolation & purification , Child , Child, Preschool , Chronic Disease , Female , Gastric Mucosa/pathology , Gastritis/etiology , Gastritis/pathology , Gastroscopy , Humans , Hyperplasia , MaleABSTRACT
A comprehensive examination of children with aggravated heredity regarding cholelithiasis revealed in the majority of them the first stage of cholelithiasis, namely abnormalities in physicochemical properties of the bile. It is demonstrated that formation of the pathology under consideration is promoted by combination of such factors as hereditary aggravation regarding cholelithiasis, complications of the perinatal period, vegetative disturbance, biliary dyskinesia, and derangement of the intestinal flora.
Subject(s)
Cholelithiasis/genetics , Adolescent , Autonomic Nervous System Diseases/complications , Biliary Dyskinesia/complications , Child , Child, Preschool , Cholelithiasis/complications , Cholelithiasis/diagnosis , Female , Humans , Intestines/microbiology , MaleABSTRACT
Cholesterol synthesis was studied in intestinal biopsies obtained from children with different forms of malabsorption syndrome. It was demonstrated that intestinal cholesterol synthesis is enhanced in celiac disease and several other forms of malabsorption syndrome. Mevinolin inhibited intestinal cholesterol synthesis in all groups of patients. No correlation was found between cholesterol synthesis and age and sex of the patients, clinical manifestation of the disease and plasma cholesterol level.
Subject(s)
Cholesterol/biosynthesis , Intestine, Small/metabolism , Malabsorption Syndromes/metabolism , Child, Preschool , Humans , Infant , Organ Culture TechniquesSubject(s)
Heterozygote , Thalassemia/diagnosis , Urban Population , Child , Female , Hemoglobins/analysis , Humans , Male , Moscow , Thalassemia/blood , Thalassemia/pathologySubject(s)
Celiac Disease/drug therapy , Cholesterol/biosynthesis , Gliadin/therapeutic use , Intestinal Mucosa/drug effects , Jejunum/drug effects , Plant Proteins/therapeutic use , Biopsy , Celiac Disease/metabolism , Celiac Disease/pathology , Child , Child, Preschool , Culture Techniques , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Gliadin/analogs & derivatives , Humans , Infant , Intestinal Absorption/drug effects , Intestinal Mucosa/metabolism , Intestinal Mucosa/pathology , Jejunum/metabolism , Jejunum/pathology , Lovastatin/therapeutic useABSTRACT
Sixteen children with vitamin-D deficiency underwent jejunoscopy with spot jejunal biopsy. Morphologic evaluation of the biopsy specimens provided evidence for glycocalyx destruction and malabsorption syndrome. Hyperactivity of hydrolytic enzymes may be concerned with the adjustment to a disturbance of the calcium/phosphorus metabolism.