ABSTRACT
Rhabdomyosarcoma (RMS) is the most commonly occurring malignant soft tissue tumor in children. Despite improving its treatment methods, the current outcome in the advanced stages of this tumor is not satisfactory. RMS cells are characterized by abnormal cellular signaling due to the changes in the activity of the tyrosine kinases. Thus, substances blocking the mitogenic signal transmitted by receptors with tyrosine kinase activity raise hopes for inhibition of the uncontrolled cell growth. In this study, we examined the anticancer activity of tyrphostin AG1296, a tyrosine kinase inhibitor that binds to the intracellular domain of the PDGF (platelet-derived growth factor) receptor in human RMS alveolar and embryonal cell lines. We have discovered that tyrphostin AG1296 completely inhibited cell proliferation and effectively inhibited cell viability. Tyrphostin AG1296 induced apoptosis of the RMS cells and significantly inhibited their migration. Additionally, investigated inhibitor slightly inhibited expression of AKT and phosphorylation of ERK in alveolar RMS cells. Importantly, the inhibitor exerted also potent effects on the nanomechanical properties and cytoskeleton organization of RMS cells. To conclude, tyrphostin AG1296 is a promising compound in the treatment of alveolar RMS. Undoubtedly, a better knowledge of receptor pathomechanism of tyrosine kinases may contribute to developing new, more effective ways of RMS treatment.
Subject(s)
Rhabdomyosarcoma , Tyrphostins , Cell Proliferation , Child , Humans , Phosphorylation , Rhabdomyosarcoma/drug therapy , Rhabdomyosarcoma/pathology , Tyrphostins/pharmacologyABSTRACT
AIM OF THE STUDY: The effectiveness of 2 aftercare treatments was examined with regard to patients' implementation of health-enhancing recommendations and changes in psychological distress, as well as mental and physical health. METHODS: Psychosomatic patients were randomly assigned to an intervention group (aftercare over the telephone or face-to-face) or to a control group without aftercare (n=62) at the end of their treatment. Patients in the intervention group (n=139) were offered up to 4 aftercare conversations during the 3 months after their rehabilitation. Data were collected at admission (t1), at discharge (t2) as well as 3 (t3) and 9 (t4) months after discharge. Patients were asked whether they implemented health-enhancing recommendations, recorded in the discharge report, at t3 and t4. Psychological distress (SCL-90-R) was measured at t1, t2, t3 and t4, mental and physical health (SF-12) at t2, t3 and t4. RESULTS: In the intervention group, more participants implemented the recommendations made at discharge such as sports and exercises or outpatient psychotherapy as compared to the control group. Likewise, participants of the intervention group maintained better their physical health. Aftercare over the telephone and face-to-face did not differ in their effectiveness; however, less time was required for aftercare conversations over the telephone. CONCLUSIONS: Aftercare over the telephone seems to be an economical approach to support patients in implementing healthcare recommendations made at discharge after inpatient rehabilitation.
Subject(s)
Aftercare/methods , Interviews as Topic/methods , Psychophysiologic Disorders/diagnosis , Psychophysiologic Disorders/rehabilitation , Psychotherapy/methods , Remote Consultation/methods , Aftercare/psychology , Female , Germany , Humans , Male , Middle Aged , Patient Compliance , Patient Discharge , Psychophysiologic Disorders/psychology , Treatment OutcomeABSTRACT
BACKGROUND: The aim of this study was to examine the influence of aftercare sessions in a psychosomatic rehabilitation on the sociomedical development. The after care group was compared with treatment as usual. METHODS: In a prospective, controlled and randomized longitudinal study data about the state of health and job status were assessed at 4 measured time points. As an indicator of the sociomedical development the current employment status was used. Aftercare treatment (n=139) included 2-4 interview contacts (via telephone or face-to-face) within the first 3 months after discharge from inpatient psychosomatic rehabilitation. A control group (n=62) received treatment as usual. RESULTS: 3 and 9 months after the end of rehabilitation participants in the aftercare showed a better sociomedical development than the participants in the control condition.
