Effect of Self- and Inter-Cultivar Grafting on Growth and Nutrient Content in Sweet Basil (Ocimum basilicum L.).

Vegetable grafting has been applied to fruiting crops, yet only to a limited extent in leafy greens and herbs which may also benefit from grafting. In this study, we examined the effect of reciprocal grafting two sweet basil (Ocimum basilicum L.) cultivars of differing vigor on plant growth and leaf mineral nutrient concentration to test whether differences in growth exist due to grafting and whether such differences are due to altered plant nutrient status in two trials. Two cultivars 'Nufar' (NU), a high vigor cultivar, and 'Dolce Fresca' (DF), a cultivar developed for compact growth, were selected. Four grafted treatments (scion/rootstock) were created by self-grafting (NU/NU and DF/DF) and reciprocal inter-cultivar grafting (DF/NU and NU/DF). Un-grafted plants (ug-NU and ug-DF) served as controls. Following grafting, plants were grown for 26 or 21 days in a greenhouse. DF rootstocks decreased NU shoot dry mass (19-29%) and stem length (12%) compared to ug-NU in both trials, while dry root mass was reduced (28%) in the second trial. In contrast, NU rootstocks did not affect DF growth in the first trial but significantly decreased dry shoot (18%) and root (31%) mass, compared to ug-DF in the second. Concentration of most inorganic nutrients examined was affected by both rootstock and scion genotype. For NU scions, DF rootstocks resulted in significantly higher (5-29%) levels of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, copper, and zinc in leaf tissue than ug-NU. For DF scions, NU rootstocks resulted in significantly higher (7-9%) levels of potassium and phosphorus but significantly lower (11-23%) levels of magnesium, sulfur, boron, copper, and zinc when compared to ug-DF. Results of this study show that inter-cultivar grafting sweet basil using a more vigorous cultivar as a rootstock did not enhance the growth of a less vigorous scion and reduced concentrations of certain nutrients. However, grafting a vigorous scion to a less vigorous rootstock reduced shoot growth but generally increased nutrient concentrations. This suggests that changes in growth in grafted basil are not due to altered nutrient status. Further research is needed to determine specific physiological processes influencing grafted basil growth.

Horticultural, systems-engineering and economic evaluations of short-term plant storage techniques as a labor management tool for vegetable grafting nurseries.

This transdisciplinary study has a three-fold systems approach in evaluating a horticultural technology: 1) horticultural evaluations, 2) economic and resource analyses, and 3) systems engineering analyses, using low temperature storage as an example technology. Vegetable grafting is a technique to produce value-added seedlings but requires labor intensive nursery operations. Low temperature storage of seedlings for a short period of time can reduce peak production, but has not been evaluated at the extent demonstrated in this paper. Seedlings of 22 genotypes of Cucurbitaceae (cucurbit family) and Solanaceae (nightshade family) were evaluated for storability under selected temperatures and photosynthetic photon flux. Storability of Cucurbitaceous seedlings varied between 2 to 4 weeks at 12°C and 13 µmol m-2 s-1. Solanaceous seedlings were generally storable for 4 weeks at 12°C and 13 µmol m-2 s-1, but tomato seedlings could be stored for 4 weeks at 10°C and 5 µmol m-2 s-1. Capital and weekly operational costs of a low temperature storage system with a design that meets environmental requirements were estimated as $671 to $708 per m2 footprint and $0.79 to $2.21 per m2 footprint per week, respectively. Electricity costs per plant was less than 0.1 cents for 2 to 4 weeks of storage. Using a schedule-optimization heuristic and a logistics simulator previously developed for grafting nursery operations, six production scenarios consisting of two crops (tomato or watermelon) and three production peak patterns were examined to evaluate the impact of including low temperature storage. While the overall average costs of grafting labor were not significantly different, maximum labor demand and grafting labor cost during the peak production week were reduced by 31% to 50% and 14% to 30% by using storage, respectively. Therefore, low temperature storage can be an effective means to address the issue of labor management in grafting nurseries.

Increasing the vegetable intake dose is associated with a rise in plasma carotenoids without modifying oxidative stress or inflammation in overweight or obese postmenopausal women.

The optimal amount of vegetable consumption required to reduce chronic disease risk is widely debated. Intervention trials evaluating biological activity of vegetables at various doses are limited. We conducted a 3-dose, crossover feeding trial to test the hypothesis that vegetable intake is associated in a dose-dependent manner with increased plasma carotenoids and subsequently reduced oxidative stress and inflammation in 49 overweight, postmenopausal women. Participants were assigned in random order to 2 (130 g), 5 (287 g), and 10 (614 g) daily servings of fresh, greenhouse-grown vegetables for 3-wk intervals with a 4-wk washout period between treatments. Plasma total carotenoids significantly increased from 1.63 to 2.07 µmol/L with a dose of 2 vegetable servings, from 1.49 to 2.84 µmol/L with a dose of 5 vegetable servings, and from 1.40 to 4.42 µmol/L with a dose of 10 vegetable servings (pre-post paired ttests, all P < 0.001). The change during each feeding period increased with each dose level (P < 0.001). Urine concentrations of 8-isoprostane F2α, hexanoyl lysine, and serum high sensitivity C-reactive protein were not affected by any administered vegetable dose. In this variable-dose vegetable study, a dose-response for plasma carotenoids was demonstrated without significant change in oxidative stress and inflammation in overweight, postmenopausal women.

Cherries and health: a review.

Cherries, and in particular sweet cherries, are a nutritionally dense food rich in anthocyanins, quercetin, hydroxycinnamates, potassium, fiber, vitamin C, carotenoids, and melatonin. UV concentration, degree of ripeness, postharvest storage conditions, and processing, each can significantly alter the amounts of nutrients and bioactive components. These constituent nutrients and bioactive food components support the potential preventive health benefits of cherry intake in relation to cancer, cardiovascular disease, diabetes, inflammatory diseases, and Alzheimer's disease. Mechanistically, cherries exhibit relatively high antioxidant activity, low glycemic response, COX 1 and 2 enzyme inhibition, and other anti-carcinogenic effects in vitro and in animal experiments. Well-designed cherry feeding studies are needed to further substantiate any health benefits in humans.

Salt in irrigation water affects the nutritional and visual properties of romaine lettuce (Lactuca sativa L.).

The influence of salinity stress on the growth, appearance, and nutritional compounds, especially phenolic compounds and carotenoids, of romaine lettuce (Lactuca sativa L.), a low salt tolerant plant, was studied. The dry weight, height, and color of the lettuce plants were significantly changed by long-term irrigation (15 days) with higher NaCl concentration (i.e., >100 mM). However, no significant differences were observed in the growth and appearance among the control, all short-term treatments (2 days; 50, 100, 500, and 1000 mM), and long-term irrigation with low salt concentration. Moreover, in romaine lettuce treated with long-term irrigation with 5 mM NaCl, the total carotenoid content increased without color change, and the contents of major carotenoids in romaine lettuce, lutein and beta-carotene, increased 37 and 80%, respectively. No differences were observed in lutein and beta-carotene contents in short-term-treated lettuce. The phenolic content of the romaine lettuce declined with short-term salt irrigation, whereas there were no significant differences among treatments exposed to long-term irrigation. This research indicates that long-term irrigation with relatively low salt concentration, rather than short-term irrigation with high salt concentration, can increase carotenoid content in romaine lettuce without causing a tradeoff in yield or visual quality.

Effect of methyl jasmonate on phenolic compounds and carotenoids of romaine lettuce (Lactuca sativa L.).

The effect of exogenous methyl jasmonate (MeJA) on antioxidative compounds of romaine lettuce ( Lactuca sativa L.) was investigated. Lettuces were treated with various MeJA solutions (0, 0.05, 0.1, 0.25, and 0.5 mM) before harvest. Total phenolic compounds content and antioxidant capacity of romaine lettuce significantly increased after MeJA treatments (0.1, 0.25, and 0.5 mM). The total content of phenolic compounds of the romaine lettuce treated with 0.5 mM MeJA (31.6 microg of gallic acid equivalents/mg of dry weight) was 35% higher than that of the control. The increase in phenolic compound content was attributed to a caffeic acid derivative and an unknown phenolic compound, which also contributed to increased antioxidant capacity. The induction of phenylalanine ammonia-lyase (PAL) activity by the MeJA treatment indicated that phenolic compounds were altered due to the activation of the phenylpropandoid pathway. Total content of carotenoids, including lutein and beta-carotene, of the MeJA-treated lettuce did not change after 8 days of treatment, whereas the content of the control without MeJA decreased after 8 days. This research indicated that preharvest application of MeJA could increase the nutritional value of romaine lettuce under determined conditions discussed in this work.