ABSTRACT
The cellular (viability) and molecular (kinetics of changes in nucleoid viscosity) mechanisms of damage to various strains of microorganisms after single and combined action of SHF electromagnetic radiation and hydrogen peroxide are compared. The conditions are found in which marked synergetic interactions of the agents are realized. The role of repair systems in formation of final products of the interactions is discussed.
Subject(s)
Escherichia coli/drug effects , Escherichia coli/radiation effects , Hydrogen Peroxide/pharmacology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/radiation effects , Radio WavesABSTRACT
In experiments with models of isogenic Escherichia coli strains, a comparative study was made of the effect of SHF of electromagnetic field and hyperthermia. The survival rate of bacteria was determined and, simultaneously, injuries to genetic supramolecular structures were registered through measuring the anomalous time dependence of cell lysate viscosity. The combined effects of the mixture of these factors with H2O2 microconcentrations were studied. The differences observed in their realization were attributed to the different mechanisms of action of these factors on the repair enzyme systems of the studied cells. It has been found that the effect of microwaves on microorganisms causes much severer damages to DNA that hyperthermia does.
Subject(s)
Bacterial Physiological Phenomena , Electromagnetic Fields , Hot Temperature , Bacteria/enzymology , Bacteriological Techniques , DNA, Bacterial/analysis , Escherichia coli/enzymology , Escherichia coli/genetics , Escherichia coli/physiology , MicrowavesABSTRACT
The comparative study of the action of microwave radiation and hydrogen peroxide, as well as their combined action, on the viability and ultrastructure of P. aeruginosa cells has been made. The combined use of microwave radiation and hydrogen peroxide has been shown to decrease the viability of P. aeruginosa 1.5-2 times in comparison with the isolated action of each factor. The electron microscopic study of the ultrastructure of cells have shown the deterioration of the surface structures, nuclear and ribosomal apparatus of the cells under the isolated action of each of the above-mentioned factors or under their combined action. The morphological picture of these changes has proved to be different. The maximum changes in the ultrastructure of P. aeruginosa cells have been registered after the combined action of these bactericidal factors.