ABSTRACT
The changes of functional state isolated by Lanhendorf old rat hearts with low content of ubiqinone--coenzyme Q (CoQ) under activation of it endogenous synthesis through administration of precursors--4-hydroxybenzoic acid, methionine and modulator vitamin E were studied. The activation of ubiqinone biosynthesis contribute to cardioprotective effect due to reduce the degree of the ischemia-reperfusion injury in old rat heart, namely the restoration of myocardial contractile function and coronary flow as well as decrease the end diastolic pressure and oxygen cost of the heart compared with control group of the animals during ischemia-reperfusion. Thus the results allow to conclude that the activation of KoQ biosynthesis under administration of it precursors has protective effect in the development of the heart postreperfusion damages in aging.
Subject(s)
Aging/metabolism , Myocardial Reperfusion Injury/prevention & control , Myocardium/metabolism , Ubiquinone/biosynthesis , Aging/drug effects , Aging/pathology , Animals , Cardiotonic Agents/pharmacology , Coronary Circulation/drug effects , Heart/drug effects , Heart Function Tests , In Vitro Techniques , Male , Methionine/pharmacology , Myocardial Reperfusion , Myocardial Reperfusion Injury/metabolism , Myocardial Reperfusion Injury/pathology , Myocardium/pathology , Parabens/pharmacology , Rats , Rats, Wistar , Ubiquinone/physiology , alpha-Tocopherol/pharmacologyABSTRACT
This research work is devoted to study of the sensitivity of mitochondrial permeability transition pore (MPTP) opening to its inductors--ions Ca2+ (10(-4) mol/l) and oxidant phenylarsine oxide (10(-4) mol/l) and content of ubiquinone (coenzyme Q, CoQ) and vitamin E in heart mitochondria of adult, old (control) and old rats under administration of precursors an modulator of CoQ biosynthesis--4-hydroxybenzoic acid, methionine and modulator of CoQ biosynthesis, namely vitamin E. The results of our research demonstrate that administration of complex of biologically active substances, which are precursors and modulators of CoQ biosynthesis, leads to decrease in the sensitivity of MPTP opening to its inductors and increase of CoQ and vitamin E content in old rats heart mitochondria. Therefore the results obtained lead to a conclusion that increase of CoQ content due to administration of precursors and modulator of its biosynthesis is an effective way in the inhibition of MPTP opening. This approach as well as application of CoQ-containing medicals may be used for correction of mitochondrial dysfunction under various pathologies of cardiovascular system and in aging.
Subject(s)
Aging/metabolism , Mitochondria, Heart , Mitochondrial Membrane Transport Proteins/metabolism , Myocardium/metabolism , Ubiquinone/biosynthesis , Aging/drug effects , Animals , Arsenicals/pharmacology , Calcium/pharmacology , Male , Methionine/pharmacology , Mitochondria, Heart/drug effects , Mitochondria, Heart/metabolism , Mitochondrial Membrane Transport Proteins/antagonists & inhibitors , Mitochondrial Permeability Transition Pore , Mitochondrial Swelling/drug effects , Parabens/pharmacology , Rats , Rats, Wistar , Vitamin E/metabolism , Vitamin E/pharmacologyABSTRACT
Ubiquinone (coenzyme Q10) is an obligatory component of the respiratory chain in the inner mitochondrial membrane coupled to ATP synthesis and acts as an antioxidant. Its additional localization in the different subcellular fractions is probably associated with its multiple functions in the cell. Coenzyme Q10 deficiency has been observed in patients with congestive heart failure, angina pectoris, coronary artery disease, cardiomyopathy, hypertension, mitral valve prolapse. The clinical benefits of Q10 supplementation in prevention and treatment of cardiovascular diseases have been observed in many trials. Ubiquinone is the introduction of the metabolic drugs and may be recommended to patients with cardiovascular pathologies as an adjunct to conventional treatment.
Subject(s)
Cardiovascular Diseases/drug therapy , Ubiquinone , Animals , Cardiovascular Diseases/metabolism , Cardiovascular Diseases/prevention & control , Energy Metabolism/drug effects , Humans , Mitochondria/drug effects , Mitochondria/metabolism , Myocardium/metabolism , Ubiquinone/administration & dosage , Ubiquinone/physiology , Ubiquinone/therapeutic useABSTRACT
In addition to well known function of ubiquinone as a component of mitochondrial electron-transport chain and antioxidant, the latest research data that evidence for its function as a regulator of the processes connected with gene expression, signal transduction, including regulation of apoptosis are considered in this review. We also discuss practical aspects of use of ubiquinone for prophylaxis and treatment of cardiovascular, oncological diseases, pathologies of immune system, disorders caused by the effect of ionizing radiation.
Subject(s)
Ubiquinone , Animals , Apoptosis , Cell Membrane/metabolism , Electron Transport , Humans , Mitochondrial Membrane Transport Proteins/metabolism , Mitochondrial Permeability Transition Pore , Molecular Structure , Ubiquinone/metabolism , Ubiquinone/physiology , Ubiquinone/therapeutic useABSTRACT
The results of study of influence of C6-analog of alpha-tocopherol on the structural and functional state of cardiomyocytes plasma membranes (CPM) in vitro are given. Under condition of incubation of CPM of intact animals the level of phospholipids was decreased, the level of fatty acids was increased, intensity of processes of free-radical oxidation (FRO) was significantly increased and activity of Na+, K(+)-ATPase was decreased in control samples of CPM. The analog of vitamin E supplementation at a concentration of 10 mg/ml of the medium significantly prevented the development of these changes. All of these can be evidence of effective membranes protective action of C6-analog of vitamin E on CPM.
